LOCUS       sequence-390147-       11096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For ubiquitous expression of split-intein Gal4-N-int component under
            control of the tubulin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, 
            White BH, Perrimon N
  TITLE     split-intein Gal4 provides intersectional genetic labeling that is 
            repressible by Gal80.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 
            10.1073/pnas.2304730120. Epub 2023 Jun 5.
  PUBMED    37276389
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-13"; volume: "120(24):e2304730120. 
            doi"; pages: " 10.1073/pnas.2304730120. Epub 2023 Jun 5"
COMMENT     Sequence Label: pTub-split-intein-Gal4[1-20]-N-int
FEATURES             Location/Qualifiers
     source          1..11096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          587..652
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             782..802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1074..1208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1111..1130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1165..1184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1237..1306
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(1525..1543)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1564..1580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1564..1580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1577..1599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1588..1604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1612..1618,1619..1636,1637..1642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1795..1812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1966..2554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2046..2065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2725..3516,3517..3585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3348..3367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3586..3690)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3716..4171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3853..3874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4065..4084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4298..4320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4312..4329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4313..4329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4336..4355
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4336..4354
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4388..4406)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4858..4929,5331..5604,5679..6333,6395..6710,6914..7045,
                     7116..7730)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    8505..8538
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 catttagcta cctctctcac tcgcacgcag tgccggctca agttgggcgc ggctctgcaa
       61 ttatcgattt tcttggggtg tgtaactaat catccgtttt cccttcctcc tcatccacag
      121 cgtgaaggta cccgcccggg gatcagatcc gcggccgcaa catgaagcta ctgtcttcta
      181 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgcctggat ctgaagaccc
      241 aggtgcagac cccccagggc atgaaggaga tcagcaacat ccaggtgggc gatctggtgc
      301 tgagcaacac cggctacaac gaggtgctga acgtgttccc caagagcaag aagaagagct
      361 acaagatcac cctggaggat ggcaaggaga tcatctgcag cgaggagcac ctgttcccca
      421 cccagaccgg cgagatgaac atcagcggcg gcctgaagga gggcatgtgc ctgtacgtga
      481 aggagtaata actcgagggt acctctagag gatctttgtg aaggaacctt acttctgtgg
      541 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
      601 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
      661 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
      721 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
      781 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
      841 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
      901 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt
      961 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1021 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1081 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1141 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1201 tctggatcca ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca
     1261 gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat
     1321 gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc
     1381 gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc
     1441 gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac
     1501 actagttcta gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     1561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     3721 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3781 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3961 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4141 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     4201 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     4261 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     4321 acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacgta ccgggcccct
     4381 agtatgtatg taagttaata aaacccattt ttgcggaaag tagataaaaa aaacattttt
     4441 tttttttact gcactggata tcattgaact tatctgatca gttttaaatt tacttcgatc
     4501 caagggtatt tgatgtacca ggttctttcg attacctctc actcaaaatg acattccact
     4561 caaagtcagc gctgtttgcc tccttctctg tccacagaaa tatcgccgtc tctttcgccg
     4621 ctgcgtccgc tatctctttc gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt
     4681 cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa
     4741 acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat
     4801 tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg
     4861 ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat
     4921 ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg
     4981 cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg
     5041 caattgcaca aacaaatagt gacacgaaac agattattct ggtagctgtt ctcgctatat
     5101 aagacaattt ttgagatcat atcatgatca agacatctaa aggcattcat tttcgactat
     5161 attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag atgcacaaaa
     5221 aataaataaa agtataaacc tacttcgtag gatacttcgg ggtacttttt gttcggggtt
     5281 agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag ggtgacagcg
     5341 gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac tacggcacgc
     5401 tccggccacc cagtccgccg gaggactccg gttcagggag cggccaacta gccgagaacc
     5461 tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg ggctccggat
     5521 ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac ataccggcgc
     5581 ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga tctgtgtgaa
     5641 atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg cctatccggg
     5701 cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc tgaatgccct
     5761 tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg
     5821 ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct
     5881 ctttatcggc tccctaacgg ccagggaaca cctgattttc caagccatgg tgcggatgcc
     5941 acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc
     6001 gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg
     6061 cggagaaagg aagcgtctgg cattcgcctc cgaggctcta accgatccgc cgcttctgat
     6121 ctgcgatgag cccacctccg gactggactc ctttaccgcc cacagcgtcg tccaggtgct
     6181 gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga
     6241 gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg
     6301 cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt attaagggta
     6361 tctagtatta cataacatct caactcctat ccagcgtggg tgcccagtgt cctaccaact
     6421 acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg gagatcgagt
     6481 cccgtgatcg gatcgccaag atatgcgaca attttgccat tagcaaagta gcccgggata
     6541 tggagcagtt gttggccacc aaaaatctgg agaagccact ggagcagccg gagaatgggt
     6601 acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt
     6661 cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt
     6721 ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag
     6781 atagaattgc ctgattccac acccttctta gtttttttca atgagatgta tagtttatag
     6841 ttttgcagaa gataaataaa tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca
     6901 ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc aacaactcac
     6961 gcaagtgggt gtgatgaata tcaacggagc catcttcctc ttcctgacca acatgacctt
     7021 tcaaaacgtc tttgccacga taaatgtaag tcatgtttag aatacatttg catttcaata
     7081 atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag ctgccagttt
     7141 ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg ggcaaaacga
     7201 ttgccgaatt gccgcttttt ctcacagtgc cactggtctt cacggcgatt gcctatccga
     7261 tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg gtcactctgg
     7321 tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc tcgacctcga
     7381 tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc ggcttcttct
     7441 tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt
     7501 acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct
     7561 gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact
     7621 tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc gtgagcttcc
     7681 gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata
     7741 tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt actgtttatt
     7801 gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata
     7861 gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac
     7921 caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca
     7981 ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct
     8041 ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca
     8101 ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc
     8161 tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg
     8221 tacaatttat ttgctccaat atatttgtat atatttccct cacagctata tttattctaa
     8281 tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac
     8341 aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat
     8401 ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata ataagaatac
     8461 atttaattta gaaaatgctt ggatttcact ggaactaggc tagcataact tcgtataatg
     8521 tatgctatac gaagttatgc tagcggatcc aagcttgcgg ccgcaattct atattctaaa
     8581 aacacaaatg atacttctaa aaaaaaatca tgaatggcat caactctaag cttgcacagg
     8641 tcctgttcga taacgtcgta ctcgggaatc acctttaact tgtccgctat tgggtatttg
     8701 tccttaaaca cattcgtaat ctctcccaca atcattgccc tggcctctgc aatgtaaaaa
     8761 gtatttataa aatatcgctg gctctaaagg aaacatatta atcaaagtat tgaggcttta
     8821 tcaatctaac atttacaagt agtcagacca caatttttag actgcacttc tgcgtaagta
     8881 aatgaaatga aatggtgacc tactcttggg tagccgtact gttatatgct gacgagcctt
     8941 cacctgggcc tgctttttct cctgtttcgt ctgatgcgtc ggatgcggct ggactggctg
     9001 attgttggga ttgggttttg gatgctgctc cgaaaggtgg gccacagctt ttgcgatggt
     9061 ggtgcccact atgcggttgc tgcaggaact acagctgaat gttcgcttta ttgtctgcag
     9121 ctcctggggc tggacgaagg ccacgccctc acccggtgcg gccatctcct gcggcaaaac
     9181 gtatgtggga ttcctgtcca agtagctctc gatgcccacc ataatgcgct ccagcaggtt
     9241 ggccaggtgc gggtctagct gcggcggatt cgtcgtctgg ttcactgagt ttagggtctg
     9301 cagaaagaag tgtgcctcca tggtaagcgg attcatctgc acgatcagcg ggctttagtc
     9361 taaaaattcg aatgatacaa actcgtactc acgtaaagca gtttgtttgg catcatctcg
     9421 gccatcgtct tattcttctt cgtggccttt gttaccggag cggctatgtt gggcgcggtg
     9481 gagcccggaa gctgaaaggt taatcgaatg cgttaacttt tctgcaactc gaaagtttgc
     9541 cgcctttgtt cgactgccaa taactgttga ttcgaaaatt cgaatcgaag cggttgaatt
     9601 tcgtagggtg gccaactaca ccaaagttcg ccggcggtgt atgcatgtga gtgtgagtgt
     9661 gacagtgggt gtacgtgtgc tttaagcgac acaatcgcac cgcgatgtac atatttttgt
     9721 agtaatgtgt gtgcattttt cgtccggatc acggctccat aaaataaatc atatcacagg
     9781 gctcagggat ttgtgagtag aagttcccgc cacttaaacg tcacttgtga aatcgccatc
     9841 gacggtcaac ttttactcac actggtcttt cgcggacgga ccgtctcaaa gtactgcctt
     9901 tctgcgttgg aaaacatcgc ctttttcgtc caaaaggagt ccccaggttc gatccgcatg
     9961 gcgttgtgcg tgcgtgcctt tcttttcaaa tgattacggc tattaacttg ggggcgttaa
    10021 gttggaaaca cgtaaattgc agactgcgat tagagtgacc atgagtagga gttcaaaatc
    10081 tcctgacatc attttcttaa aacctgcttt gttttttaca tttctattta atataactcc
    10141 tatttgaata aaaaaacaaa acaagtttag atgttaagat attaactaca tcctttgctc
    10201 caaagggaga ggggaagtta tggagttaat taatttgctg ttggaaatca atatggagtc
    10261 agaaatataa tgatttacta aaccttattg aatcggtaac gatgcgaatt tatattaaaa
    10321 tagcttttat gaaacattca acaaaaatat tattaatgtt ggcccacttt agcaaccggt
    10381 taggtctacc ggttgggcaa gcaaagattc acgccctggt tcgagtccca actagtcctg
    10441 caaaataccg cagcaagttt tagagagacc aagtgccatt acctctccca cttcagttat
    10501 cggttatgcg gcgtttaagt cgacagcttg ccgtctctag ctccggtgcc tatataaagc
    10561 agcccgcttt ccacatttca tattcgtttt acgtttgtca agcctcatag ccggcagttc
    10621 gaacgtatac gctctctgag tcagacctcg aaatcgtagc tctacacaat tctgtgaatt
    10681 ttccttgtcg cgtgtgaaac acttccaata aaaactcctg caggtgagta ctttaaaaaa
    10741 aaatctagtg aaataatgct gaaaagaaat ttgtgtgggc aaaattcaat gggcaaaaac
    10801 gcgatgcggc tttttctcaa aatggcggcc ggcctgcgtt ttttcctcaa aagtgatgac
    10861 gtcatgcctg tttttttttt tttgttcgca atgaggaatg gctcttaaaa tctactagat
    10921 aaaaaaaata ttcattattt ctatgctgct ggaacgcttc attaatctta aaaattctaa
    10981 attcggttac catgatactt cgacgcataa ctgtagattt tggatagaat taaagagaaa
    11041 atggcgagag agtaaaattc cggcgtcggc aaagtagagc aaaaaaatca gtatac
//
