LOCUS       sequence-390161-       11586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Capelluto F, Alberico H, Ledo-Hopgood P, Tilly JL, Woods DC
  TITLE     Lineage-Mismatched Mitochondrial Replacement in an Inducible 
            Mitochondrial Depletion Model Effectively Restores the Original 
            Proteomic Landscape of Recipient Cells.
  JOURNAL   Adv Biol (Weinh). 2023 Jan 18:e2200246. doi: 10.1002/adbi.202200246.
  PUBMED    36651121
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Biol 
            (Weinh). 2023 Jan 18:e2200246. doi: 10.1002/adbi.202200246."
COMMENT     Sequence Label: iYFP-Parkin Plasmid
FEATURES             Location/Qualifiers
     source          1..11586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        163..542
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    973..1330
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1741..1763
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1779..1795
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2133..2149
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     polyA_signal    2297..2431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4037..4747,4748..4750,4751..4753))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4079..4100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4428..4447
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4687..4708
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(4760..5127)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    4885..4903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4921..4939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4957..4975
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4993..5011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5029..5047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5065..5083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5101..5119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5144..5654
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5573..5591
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5673..6419
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        6437..6766
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6617..6752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6775..7374
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6775..6794)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    7391..7794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    8814..8935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8958..8975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8958..8974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8967..8989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9183..9202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9302..9324
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9362..9380)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9448..9552
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9553..9621,9622..10413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9771..9790)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10584..11172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11073..11092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11326..11343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11460..11481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11496..11501,11502..11519,11520..11526)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11534..11550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11539..11561
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11558..11574
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11558..11574
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat ataaagcatt
       61 tgacttgttc tatgccctag gagcttggcc cattgcatac gttgtatcca tatcataata
      121 tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta
      181 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      241 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      301 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      361 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      421 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      481 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      541 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      601 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      661 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcatc tagagcgctt
      721 ctgctccccg agctctataa aagagcccac aacccctcac tcggggcgcc agtcctccga
      781 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
      841 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
      901 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
      961 ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
     1021 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
     1081 ccgtggtgga actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga
     1141 cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa tcccgatcgt ttaggactct
     1201 ttggtgcacc ccccttagag gagggatatg tggttctggt aggagacgag aacctaaaac
     1261 agttcccgcc tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc
     1321 ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
     1381 aaaatgaggg cccgggctag cctgttacca ctcccttaag tttgacctta ggtcactgga
     1441 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
     1501 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
     1561 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
     1621 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
     1681 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
     1741 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
     1801 ctctaggcgc ccccatatgg ccatatgaga tcctatatgg ggcacccccg ccccttgtaa
     1861 acttccctga ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac
     1921 aggctctcta cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac
     1981 aactggaccg accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc
     2041 gacaccagac taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca
     2101 cccccaccgc cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg
     2161 ctgccgaccc cgggggtgga ccatcctcta gatcataatc agccatacca catttgtaga
     2221 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2281 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag
     2341 catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa
     2401 actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtctt
     2461 aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca gtagctctga
     2521 cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat tggctgtcca
     2581 gcttagctcg caggggaggt ggtctggatc cttacacgtc gaaccagtgg tcccccatgc
     2641 agacgcggtt ccactcgcag ccacagttcc agcaccactc gagcctgcac tggggctgcg
     2701 gacacttcat gtgcatgcag cctccatttt tttccactgg tacatggcag cggggacagg
     2761 gcttggtggt tttcttgatg gtttctttgg aggctgcttc ccaacgagcc tgctcggcgg
     2821 ctctttcatc gactctgtag gcctgagtag ttgttcctga ggcttcaaat acggcactgc
     2881 actccccttc atggtacgct tctttacatt cccggcagaa ggcaaaccca cagcccaggc
     2941 cattgccccc ttcgcaggtg actttcctct ggtcaggctc cggcagcagc cccgctccac
     3001 agccagggcg ggggcataac acgcccccca tctgcaggac acactcctct gcaccatact
     3061 gctggtaccg gttgtactgc tcttctccca gaatcctgaa gtgatggagc tctttaatca
     3121 aggagttggg acagccagcc acacaaggca gggagtagcc aagctgaggg tcgtggacaa
     3181 actgccgatc attgagtctt gtcacacagt ataagtggaa acagtctaag caaatcacgt
     3241 ggcgggagtt gcactggaaa accaggacgg ggctcctgac gtctgtgcac gtaatgcaag
     3301 tgatgttccg actatttgtt gcgatcaggt gcaaagctac tgatgtttcc ttgtcagagg
     3361 tggggtgtgc tccacattta aagaaaaatt ctgcactagt cccagggcag tgtggggatt
     3421 ggcattcacc actcatccgg tttggaatta aaacatcatc ccagcaagat ggaccctggg
     3481 tcaaggtgag cgttgcctgc ctgcaggtgc tgcactgtac cctgagtttt cccggctgca
     3541 ctctttgaca ggggcctttg caatacacat aaaagctgtt gtagactgat ctacctgctg
     3601 gacttccagc tggtggtgag tccttcctgc tgtcagtgtg cagaatgaca gccagcccca
     3661 cagagtctcc tgggaggact gagctgctga ggtccacccg agtcaagctc tggggctccc
     3721 gctcacagcc tcccgccgcg tttctggggt cgtcgcctcc agttgcattc atttcttgac
     3781 cttttctcca cggtctctgc acaatgtgaa caatgctctg ctgatccagg tcacaattct
     3841 gcacagtcca gtcattcctc agctccttcc ctgcgaaaat cacacgcaac tggtcagccg
     3901 gaaccccctg tcgcttagca accacctcct tgagctggaa gatgctggtg tcagaatcga
     3961 cctccactgg gaaaccatgg ctggagttga acctgacaaa cactatcaaa gcttgagctc
     4021 gagatctgag tccggacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     4081 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
     4141 ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     4201 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     4261 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     4321 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     4381 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     4441 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     4501 agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg ccgtagccga
     4561 aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     4621 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     4681 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     4741 ccttgctcac catgaattct ttacgagggt aggaagtggt acggaaagtt ggtataagac
     4801 aaaagtgttg tggaattgaa gtttactcaa aaaatcagca ctcttttata ggcgccctgg
     4861 tttacataag caaagcttat acgttctcta tcactgatag ggagtaaact ggatatacgt
     4921 tctctatcac tgatagggag taaactgtag atacgttctc tatcactgat agggagtaaa
     4981 ctggtcatac gttctctatc actgataggg agtaaactcc ttatacgttc tctatcactg
     5041 atagggagta aagtctgcat acgttctcta tcactgatag ggagtaaact cttcatacgt
     5101 tctctatcac tgatagggag taaactcgag gtgataattc cacggggttg gggttgcgcc
     5161 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     5221 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     5281 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     5341 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     5401 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     5461 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     5521 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     5581 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     5641 acctctctcc ccagggggat catcgaatta ccatgtctag actggacaag agcaaagtca
     5701 taaactctgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
     5761 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     5821 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac tcctgccccc
     5881 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     5941 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     6001 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     6061 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     6121 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     6181 tgaaacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     6241 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     6301 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     6361 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     6421 taagtaagaa ttgaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6481 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6601 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6661 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6721 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaacgc gaccatgacc
     6781 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     6841 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     6901 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6961 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7021 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7081 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7141 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7201 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7261 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7321 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt
     7381 aattcctgtt aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa
     7441 ctatgttgct ccttttacgc tgtgtggata tgctgcttta atgcctctgt atcatgctat
     7501 tgcttcccgt acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7561 tgaggagttg tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc
     7621 aacccccact ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt
     7681 ccccctccct atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg
     7741 ggctaggttg ctgggcactg ataattccgt ggtgttgtcg gggaagctga cgtcgagatc
     7801 ttcctgcgga gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     7861 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     7921 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttctcgcttc tgttcgcgcg
     7981 cttctgctcc cagacatcaa caaaagagcc cacaacccct cactcggggc gccagtcctc
     8041 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     8101 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     8161 ggggtctttc acgcgtatgc cgcatagtta agccaccgcg gtggcggtct ggaccacgcc
     8221 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8281 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8341 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8401 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8461 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     8521 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     8581 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     8641 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     8701 tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     8761 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     8821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8941 gcgcgtcgaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9001 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9061 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     9121 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9181 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     9241 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     9301 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     9361 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     9421 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9481 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9541 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9601 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9661 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9721 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9781 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9841 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9901 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9961 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10021 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10081 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10141 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10201 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10261 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10321 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10381 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10441 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10501 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10561 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10621 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10681 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10741 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10801 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10861 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10921 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10981 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11041 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11101 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11161 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11221 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11281 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11341 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11401 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    11461 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    11521 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    11581 tacgcc
//
