LOCUS       sequence-390232-        5512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the CEP neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP097
FEATURES             Location/Qualifiers
     source          1..5512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2570..2719
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3257..3361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3362..3430,3431..4222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3580..3599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4393..4981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4882..4901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5135..5152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5269..5290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5305..5310,5311..5328,5329..5335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5343..5359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5348..5370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5367..5383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5367..5383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcctgct gatccaatgg caaggtcgtt
       61 aactgaaaaa taaattattt tgaaaattgt gtaagtagac tactgaatta aaaaacaact
      121 cacgactttc tcctgggagc ccattttctc tcattgagaa tgctgcgagc atgattagaa
      181 cgatgatgca attcttcatg gcgaagtgac gatctttggc tgtgcggccg gcttttattc
      241 ggacgatttt ttagtatccc accaaaaaaa atgtaattgt tgggaacaat accgtccgaa
      301 tttctatatt gttccccccg cgtcgaattt ttataacact tgttgtttgg tttcttggtc
      361 ttttttgcaa aaccgtcgat tattgcagag attttagttg gagcggaata acggaacgat
      421 attaaaaaac ccgtggagat ggagaaatca cagaaaaata ttacggttta cttatcagag
      481 gctcgactag gaaattttgt agtactttta aaattaattt taaaaacatg aactgatcga
      541 aaaaatgaac aacaatatat atatatataa gtttttcaat tattaattta atattaaaaa
      601 tgagaaattt ctaataagaa tttttcagaa acaatttttc caaatctcct tgtactgaaa
      661 atatgtgaaa caacttgaag atttttaaag ttgatattag ccgttttctt tcaagctttt
      721 gaaaatgtta taaatttgga aaaaaaacat cgacaaattg caatttttat ttgaaaaaaa
      781 attcttagga acctatttga caggtctaaa taatttcttt ttgtaaaaaa tgaaaacttt
      841 tgatattttt tgaggagccc tgaaaaatga aattttccat aaagctacct agacagcctt
      901 aaaatcatca aaaacctgca aaaaatcaca ttttgagcaa ttttacgagc ctctggtgcc
      961 cggcctcata cttgaccaca aacaaaaatt acagacattc tgattcttta cgtgcaaaac
     1021 cacccgatat tttctttttt tttccggaat acatcaaaca ttgcattggc attttccggt
     1081 atttacatgc agccctccgc aacaaaaaca atattatagc aatataataa tctgcggaag
     1141 caatttttca tttataccct accgtaattg ttttccggtg ggagggataa tagctgggtt
     1201 attggaaagg ttacgtataa aagaccccgc tcgtatgtgt cattttcacg atttgtcgcc
     1261 cgttttatct gaagcaaaag aagaagctgt cattttcaga agcgatgagt ggtgagtttg
     1321 aagtggggcg atataggggg gcacaacctt tttcgatttt catttcatat tctatactac
     1381 tcaagtcaat tgcgcataat atttcccccc gctctcaggc aggcagccca gaaaaaaatg
     1441 aatttgttcc gccatattcc actgagcaac tcccaactta atggagtcaa tcatggtaaa
     1501 tttttcgtcg aaaaatttat gatagggtgg gaactaagtg gttggaaaaa gaaaagtgcg
     1561 cttttttcat tttttacttt tcatttttta tacatttttg gcttttggcg cttggaaaag
     1621 tagtaaccat gaactttttc tcgaatttga gactcaacaa cgacttgtta gataatgtga
     1681 attctggtaa gtggttagga tttttttgaa ttaatttaat aacctattat acctaatata
     1741 acctaatata gcctaataac ctaatataaa ttaacctgaa aaattgcaaa agttgtatag
     1801 catgccgagt tgctggagct accaagatcc tgaaaaatct ccactttgcg aaattacagt
     1861 ttcagaatct ttttatggtt ccccagtatc tgaaaatacg gaaattttaa tatttcagta
     1921 gctaatccgt taccaaaatt ttgctaaata agtcaccgtt tttccaaagt cacggccgtt
     1981 ttagtcgtct tacagacggt cgaatttttt tttaagtaga gtggcaactt gatatatcta
     2041 ctcgtgtaga taataagtca tcaaattcat caaaatctta cccctcggca gacgtcttgt
     2101 ttatataacc aaaactgcca aactttggca ctttaacagt tgggctcaat tttgactaaa
     2161 caatacagtt tttttcaaac aaaacatacg ctctaaaatc caaaaaaaaa ttgcaacaac
     2221 ttccagctct attccaagaa gtcaagggcc gtcagcagga ctttggcgcg cctctagagg
     2281 atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
     2341 aacattttca ggaggaccct tggctaggat gggccctaaa aagaagcgta aagctagcat
     2401 gatgttgaag aagattctca aaattgaaga actggatgag cgtgagctca tcgacatcga
     2461 ggtctccgga aaccacctct tctacgccaa cgacatcctc acccacaact cctcctccga
     2521 tgtactgcag tctggtggcg gaggggctcc taggggttcc ggacgggctg acgcattgga
     2581 cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct
     2641 tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga
     2701 tttcgacctg gacatgctga ttaactaagg taccgaattc gctagccggc catacaagta
     2761 atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt
     2821 ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa
     2881 ttcaatccaa atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc
     2941 tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt
     3001 ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac
     3061 acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa
     3121 atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg cgaacgatga
     3181 gaattgtcct cgcttgggcc caaaaggcct tttttttttg gcgccgacgt caggtggcac
     3241 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3301 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3361 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3421 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3481 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3541 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3601 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3661 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3721 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3781 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3841 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3901 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3961 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4021 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4081 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4141 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4201 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4261 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4321 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4381 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4441 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4501 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4561 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4621 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4681 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4741 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4801 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4861 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4921 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4981 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5041 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5101 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5161 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5221 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     5281 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5341 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     5401 gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa
     5461 atggctgaaa tcactcacaa cgatggatac gctaacaact tggaaatgaa at
//
