LOCUS       sequence-390233-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ADE, CEP, and PDE neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP018
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2638..2787
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3325..3429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3430..3498,3499..4290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3648..3667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4461..5049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4950..4969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5203..5220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5337..5358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5373..5378,5379..5396,5397..5403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5411..5427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5416..5438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5435..5451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5435..5451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccgagc ctatgccacc gatttgtaca
       61 aatggaaatg ggttccttga tatcaacagt caagaacatg agaataattt aaaggtaatt
      121 caaatctcaa atcgaataaa gaatgaaaaa ctatattttt caattctaga aaatatctgg
      181 aaaattttcg ccgactgttt tcaaaaaagg acctttcaaa actattttaa tagatacaac
      241 ggaagtaatt aaagcaaatt ttggcgacga tttcaaaact ttaatcacta cggcgtggtt
      301 aaaaaataca aaacaaagta tatcaaaaat gctgatattt tcagagagga tacgaatcgg
      361 atagctcaga cgtgtctgga gtcagccact gctccgatgc taaaagacga cgaggtcgtc
      421 cacgtaaaga tgaagaagca tatcgactag aaatgacgcc accgacaatg aacgaagtca
      481 ttcggtggaa tccggacaga atcgatgtga acacccgttt tataacggcg acaaaaattg
      541 cgcaagtgat gggaatgccc ccgtcgattc tgttcaacaa gtatccacga atgtttagag
      601 taagtaactg gaaatcggca attttcaaaa actgttctac cgttttagta ttcctgcgat
      661 gaagatgaca aaaatattct acatgagcaa aatcttctca tcagggcacc cggtagatgc
      721 tatcttctgg ttgcagaaga tgctcgtcag ctagttccct ccacttactt gtaagttttt
      781 tctacaaatt taaaagagaa aaccaaagaa aaatataaag gaaatgatta tcctactcca
      841 acaaaactct ccgaaaaata attattgctc taaatcgcat cgcgcttcgc cgagcagcac
      901 ttccctcacc taccgatcaa aaataacaga ttacaacgaa acaggtagaa aagaagtaga
      961 aaatatctag aaatgagaga aaaattggtt gataataaaa aaaagtccgt aaaaagatag
     1021 caattaaaat tagagcgatt gtccacgaga aaattgtgag aatattcaga gagttctata
     1081 aatttttgaa aatttagcat ttttatttca gccaagatgt cctcaacgtc tcattcctca
     1141 tctccgagcc actgctatca aaaattcgcc aaaaagcggc atcaacctac gagaagtaca
     1201 aagtctttct gcccacacaa ccgaataatt atctctgaaa tgtttctagt cgtttttgta
     1261 ttttaaagca cattcccaat tctgaatact tttctgaaat ccatgaaatg gaacttgaat
     1321 ccagttttca ctaaaacgac ctcatacact ttctctcgta tcctcaaaat atctatgaca
     1381 ttatcattag cttcgctagt ttcatttctt tcaaatatta tgcattctta aattccgata
     1441 cccgcgtgca aaagtgctct attgagcaac tttgggatca tatgtacaca ccaatgccct
     1501 tttcccaaat cttttcctgt ccttttctct aaaaacaata aatccatgcc tattccagta
     1561 tgaccccttt gaagcagata taatcgcaca aacatataca catagctcgg ataaatgtag
     1621 aaaaagaaga aaagaagtat aagtagatag atgctttccg gcaattatcc accgcaccgt
     1681 agtcttcacc aactgagact gcgtcgttag gagacgccga catgattcag aagcagaatt
     1741 tggaagaaaa acgacgatga tattgaggct ggcacacata caccggaata ttcgacatgc
     1801 caccacatct agattccaag gcaatctcta cctcttccca ttctttcggt ttttttgttc
     1861 tgacaagaaa agtggatagc tacgggctca atgagctgat tttattttta aatatcttaa
     1921 aactatacta gattcatgtg ttttcaggtc catattccaa attagtcgaa aagctgatcc
     1981 cgctacggtt tactcgaatc tcaacggcgc gcctctagag gatccccggg attggccaaa
     2041 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc
     2101 ttggctagga tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg
     2161 gaacaagctt gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag
     2221 tgtgccaagt gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc
     2281 ccactgacca gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg
     2341 tttctgctca tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac
     2401 gaagtgaaag taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct
     2461 atggatagct tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag
     2521 actgcgacat catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg
     2581 ccggaattcc cgctgcagtc tggtggcgga ggggctccta ggggttccgg acgggctgac
     2641 gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt
     2701 gacatgcttg gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc
     2761 cttgatgatt tcgacctgga catgctgatt aactaaggta ccgaattcgc tagccggcca
     2821 tacaagtaat ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa
     2881 tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa
     2941 aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat
     3001 tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat
     3061 taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat
     3121 aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa
     3181 acataaaaat atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg
     3241 aacgatgaga attgtcctcg cttgggccca aaaggccttt tttttttggc gccgacgtca
     3301 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3361 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3421 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3481 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3541 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3601 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3661 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3721 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3781 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3841 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3901 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3961 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4021 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4081 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4141 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4201 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4261 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4321 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4381 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4441 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4501 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4561 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4621 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4681 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4741 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4801 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4861 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4921 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4981 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5041 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5101 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5161 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5221 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5281 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5341 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     5401 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     5461 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
     5521 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
