LOCUS       sequence-390234-        6102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ALA neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pHW489
FEATURES             Location/Qualifiers
     source          1..6102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3160..3309
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3847..3951
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3952..4020,4021..4812)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4170..4189)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4983..5571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5472..5491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5725..5742
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5859..5880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5895..5900,5901..5918,5919..5925)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5933..5949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5938..5960
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5957..5973
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5957..5973
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctcttacc taaaacccac aaaattcgta atttgtagac accaaagcag
       61 taatttttaa ttgcgtgtaa aatcaatagt atatctcaaa aatcatccaa ccactttttc
      121 ccgaataaaa ctcccatttc tgagtacata ctcaactacg agcacacccc ggggagtcct
      181 ttcaaataat tttttcgtgg ctcaaagtgg ttttgaaaca aaatgatgtg aagagcaatt
      241 aaatgtattt tgtttgtttt ttctctcaat ttttgtttca aacgtttgtg ccagcttcag
      301 gaacttgcga aacggctatg tacatctgat aaacaagtga gagtaattag atgatcccgg
      361 aagtaagaaa ttaattgttg atctcgatta gagatagaaa attaaaaaaa gaatgtgcct
      421 cttttcagag aacggtcatt tttttccgcg gttttagcag ggtttttttt caaggttttg
      481 aaagtgaaga tatttctggt tatcaagttt tctttatgga aaaatgtttg aaaatttgac
      541 aaaaggtatt ttaaatcaaa tatagcaaaa aatccacaaa aaaaattcga ccaggttagc
      601 cagcaaacgc aaaaatattg cgcgggaatt caaatttgat ccggccgtta ctttgattgt
      661 ttgcaaaaaa aaatcattta aaaaaatcat ttaaaaaatt tagaaaatta tttacatttt
      721 ttttttacgg tcattaaatt ttttttgact ctactccacc ttaaagatta acgaagtttc
      781 taataacaaa aatttttgaa atacagtttc actagtttcc tatctgattt atcggcaaaa
      841 cttttcataa tttacttcca gaataataat aatgacatca ttttactttc cgcccaataa
      901 aatatttgaa agtttgaaaa agtgggcggg gggatgacac ccaccttcgg agaaaagagt
      961 tctagacatg ttgtacgtaa agtttcaatt tcaatttccg tttttttttc aatttcaaca
     1021 cccataaaat gggatggata agcacttggg gtcaagtttg aagtaattga tttcctccct
     1081 tttttgaaat tgtttcaaga caaaattttg tttcccgcga agagatttga attttccgct
     1141 taatttttaa aactgttcat ggaatcgtcc aacatttttt ggagtttttt ttcaaaaatt
     1201 acagcattgg tggtagtggc agcagatggc aaaaattttt aggaattgat ttttttttta
     1261 gtcctttctt atttttaaga attttataaa ttttccagtt ggatctgatt tttgaattcc
     1321 aaaaaaatat tgactcaaaa attctaaatc gggaaaaaaa ttaaaaattc ctttcccatt
     1381 gggtggaatt tcaaatgttt tactgaaata ccaattgatc attctcgaga ttttttcatg
     1441 aaaaaagtag acactgagac aatgataaaa atacgtatgt ttttgaaaaa atacatgacg
     1501 tatgcactta aagatggagt acagtttgtg ggtattttgc ttaaatagac taaaagtggg
     1561 ccaaagtcac ctaatttcat aacgagaaaa cacaaaaaat gttgaaaaat tttttatggc
     1621 ggatcagaaa aaaagagcca aattttgcta aaatctgaaa ttttgcacac gtttcagctt
     1681 tccgaacaaa ttcgtttcaa tttcatttca aaatccaata aatccccaaa tatcaaagag
     1741 caacttctga caaaattaga gctggagcac tttgaaaaat gagaaattaa ttcgaattcg
     1801 cccaaaatta aatttaaaga gacctttagc tctactgttt ctaatttatc ttagtgcttt
     1861 tagttcaaat agatcacatc actcgtatgc cgcctaaatt ttagtataaa agcagacgag
     1921 agtcatggat tttagaccac gcctactact tggctttgtg ttttttttta taatcgtttc
     1981 gcgttttttt aaactgaatc tgaaactaaa gtttttaatt tatagaaaaa aaaaatttat
     2041 cagttgcatt tcatgaaaaa aatttttccg tttttccaaa aattgctata atttaatttt
     2101 gcaccaattt ttttccaaac taaattttga atattcccca aaatttagcg aaattaaaat
     2161 ttatattacg ctctagaatt ctttaaaatt ttgaaattag aatttcgcgg caaaatatgt
     2221 attctaaaaa ttcaaatttt cattttgcgt cgatgttaga tgtttatata atagaacaca
     2281 actaagaaat attcagataa aatgtataac ttcgacataa attgggttca tttttattac
     2341 gtcagaaagg tttccggtta aggctccacc ctttttttta aatagatccc cgatgttccg
     2401 ctctgagctt cttccccatt tcttgaattt ttccgtttat ttcattataa tttacataaa
     2461 aatggcagtt atagtgctta atttttacag aaaacttgcc tcctccaaat tttcaactat
     2521 cttgaaaggc gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt
     2581 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag gatgggccct
     2641 aaaaagaagc gtaaagctag catgaagctg ttgtcttcaa tggaacaagc ttgcgacata
     2701 tgccggttaa aaaaactcag gtgctcgaag gaaaagccca agtgtgccaa gtgtctgaag
     2761 aacaactcgg agtgccgtta ttctcccaag gctatgaggt ccccactgac cagggcgcat
     2821 ctgacagagg tagaatcaag gttggaaaaa ttggaagatt tgtttctgct catgtttcct
     2881 cgggaaaacc tggacagcat tttgaacatg gactctttgg acgaagtgaa agtaatgttg
     2941 aaacaattat atatgcagga ctatggtgac acggatgctg ctatggatag cttgacccct
     3001 atggaaaatg gcatgtctga agggccgaga aaacacatga agactgcgac atcatcgcca
     3061 aaagtggatg aagatggaag ccaaagtcag ttgagtgtat cgccggaatt cccgctgcag
     3121 tctggtggcg gaggggctcc taggggttcc ggacgggctg acgcattgga cgattttgat
     3181 ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat
     3241 gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg
     3301 gacatgctga ttaactaagg taccgaattc gctagccggc catacaagta atccggatga
     3361 tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta
     3421 ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa
     3481 atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga
     3541 acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt
     3601 ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca
     3661 acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag
     3721 gaggaaggaa aactagtaaa aaataagcaa agaaattagg cgaacgatga gaattgtcct
     3781 cgcttgggcc caaaaggcct tttttttttg gcgccgacgt caggtggcac ttttcgggga
     3841 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3901 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3961 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4021 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4081 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4141 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4201 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4261 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4321 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4381 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4441 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4501 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4561 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4621 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4681 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4741 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4801 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4861 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4921 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5101 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5341 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5641 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5701 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5761 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5821 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5881 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5941 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa
     6001 acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
     6061 tcactcacaa cgatggatac gctaacaact tggaaatgaa at
//
