LOCUS       sequence-390235-        5582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ALM, PLM, AVM, PVM neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pDO010
FEATURES             Location/Qualifiers
     source          1..5582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2640..2789
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3327..3431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3432..3500,3501..4292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3650..3669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4463..5051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4952..4971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5205..5222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5339..5360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5375..5380,5381..5398,5399..5405)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5413..5429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5418..5440
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5437..5453
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5437..5453
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccacta cttgagaggt ccggcaactt
       61 gtttgaccac ttttgcaaat ttttgatgct tttctggata taattaggtg accattcttc
      121 aggtagggta aacattctgg aagctaggtt ttataagatg aaaacaaaac acacttgcca
      181 cgaagcttcc agaatgtttg taaaaatcta atttggaaga gttatatttg tgagcggaaa
      241 taatttcctt ataattttca gcttgtaaaa tcattcacat ctaataaaaa gcaaattagg
      301 taaactataa aattaacaaa taaattacca ctgcttgaag tgtagatgtg ataagaattg
      361 gtagagcaaa gacggcaaga actgcaacaa atacagcaac tgatgacgag aaaacggcca
      421 attgacgcat tcgtcgcgct aacagttcta gatctttctc attacccttc attttctgaa
      481 attactatta taaatgtgtt agccaagagt ctcccattga tcacagaatg gcaaactcaa
      541 attataaaat atatctgaaa cagattaaat gttattgtat aagatttaca tttttcaagt
      601 tttaatttcc attattgttt ccgtttttct gtcaagaaat ctatagatgt gagcatcaaa
      661 tttttacaat aagtttgttg gagagacttt taaaaatgaa tacaataatt gaatttttct
      721 tcaaagtgag gaaaaaagta aatgttgtat ccgaatgaaa gttcaataca ttgaaaaata
      781 atgtgaatga aaagcattca aatgttcaga ttttcgttca atacgaggct aacagcataa
      841 aaaaaagaat acgcgttatc acagtgtttt aagtgtaaaa gagtgacgaa gtctaatgaa
      901 aaggtatgcg tagtttacaa tggataattt gggtgtggag aagttagaat gatgtctgct
      961 gatgttaatc acttgaattc ttgttttctg atctgcaaaa gcttcttctt gaaatggttc
     1021 tcgttcttga tattcgagat agctttgaaa attccaaatt tttgacaaac atctagaaat
     1081 ttatttgaag atgaagcagt gtctgtagaa atagtaatca tgttttcaat atttatcatg
     1141 ttttcaatac cgtccaacat ggccaaatat tataaaaaac tctgaagtgt aaaaaaatat
     1201 ttttctattt ttgaaaattt tggcaaattc ccaattttcg agaaatattg gtttttaaaa
     1261 aataatttta gagcaatgtc gcatttttga gcatctacaa tgttcagatg caaataatta
     1321 aaatataaac ttccaaaatt aaaagtgaga ggaactgagt ggttgacact tttggctgta
     1381 aaattagcac tatgtagaag agtggtgttg aaaaacacaa agtgtcttgt aaagaaatct
     1441 acagtttcag aaaacctgga tattcaaagt ttctgaaaag taccactcaa attgctccta
     1501 gaaataattt taaaaaacga ttctccattt tctgataaaa tttcaagtta cggaatgttt
     1561 caaattctaa atgaatctga ttatttttct tgctacacga atctgaaaat ctaaaaatag
     1621 tctttttcaa agttccaatg aaatttccag agatctcgaa tttagatttt ttggaacagt
     1681 aattaaaaat gtttatctgt ttgtgactct tccaacttct tccgcgtttg tgttttcctt
     1741 tctaaattct gccaaaaaag aaaaagatca tccgaatgtt tcagaaatga agagtcgtca
     1801 agaaaagaaa taatgccaaa cactgatata aaattcatag aaaagtcact ttgtatactg
     1861 tatgaattcc gaagaaattg aaaggaattc tccgataaga gcaggctcac caaaaatgca
     1921 tgagaatggg gaggaaatgt gggaggagga gagctgagat gaagagaagc tgcttcaccg
     1981 atggatcagt tgtgagacga ttcgatcggc gcgcctctag aggatccccg ggattggcca
     2041 aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac
     2101 ccttggctag gatgggccct aaaaagaagc gtaaagctag catgaagctg ttgtcttcaa
     2161 tggaacaagc ttgcgacata tgccggttaa aaaaactcag gtgctcgaag gaaaagccca
     2221 agtgtgccaa gtgtctgaag aacaactcgg agtgccgtta ttctcccaag gctatgaggt
     2281 ccccactgac cagggcgcat ctgacagagg tagaatcaag gttggaaaaa ttggaagatt
     2341 tgtttctgct catgtttcct cgggaaaacc tggacagcat tttgaacatg gactctttgg
     2401 acgaagtgaa agtaatgttg aaacaattat atatgcagga ctatggtgac acggatgctg
     2461 ctatggatag cttgacccct atggaaaatg gcatgtctga agggccgaga aaacacatga
     2521 agactgcgac atcatcgcca aaagtggatg aagatggaag ccaaagtcag ttgagtgtat
     2581 cgccggaatt cccgctgcag tctggtggcg gaggggctcc taggggttcc ggacgggctg
     2641 acgcattgga cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc
     2701 ttgacatgct tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg
     2761 cccttgatga tttcgacctg gacatgctga ttaactaagg taccgaattc gctagccggc
     2821 catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg
     2881 aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct
     2941 aaaaaattaa ttcaatccaa atctaaacat ttttttttct ctttccgtct cccaattcgt
     3001 attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc
     3061 attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa
     3121 ataaggacac acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga
     3181 aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg
     3241 cgaacgatga gaattgtcct cgcttgggcc caaaaggcct tttttttttg gcgccgacgt
     3301 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3601 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3661 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3961 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4141 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4321 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4381 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4441 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4501 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4561 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4621 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4681 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4741 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4801 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4861 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4921 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4981 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5041 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5101 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5161 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5221 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5281 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5341 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5401 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5461 acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca
     5521 gagcttaaaa atggctgaaa tcactcacaa cgatggatac gctaacaact tggaaatgaa
     5581 at
//
