LOCUS       sequence-390236-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASE neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP061
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1096..1245
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        1783..1887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1888..1956,1957..2748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2106..2125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2919..3507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3408..3427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3661..3678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3795..3816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3831..3836,3837..3854,3855..3861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3869..3885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3874..3896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3893..3909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3893..3909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccggta tgttcagttg acatcgacca
       61 aggtccgcta tttagcaatc gtatcacttt ttgtcgtagt tgtattttat ggaaagtcat
      121 aagagttgaa aatattttat ttacaaaaag gtaactttta aacggaagtt ttcaaacatt
      181 tttgcaattt caccaacttc cgtgaatcca agcttttcat tattaagcaa attcaagtat
      241 aataacaact ataaataaga ccaccaaatt ttgatatccg gattaaaatg ttatttttga
      301 atagagaagt ttgatgcaga taccaacaag attaaaactt caaacattaa gcaagaagcc
      361 cccaaatgcg tatggtttca aagaaaaagg tgcaaaattg ggaagataat ctcgtttaag
      421 aaaagtttgg aaatctatta agaccaaccc ccagaagtag tagggcgcgc ctctagagga
      481 tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
      541 acattttcag gaggaccctt ggctaggatg ggccctaaaa agaagcgtaa agctagcatg
      601 aagctgttgt cttcaatgga acaagcttgc gacatatgcc ggttaaaaaa actcaggtgc
      661 tcgaaggaaa agcccaagtg tgccaagtgt ctgaagaaca actcggagtg ccgttattct
      721 cccaaggcta tgaggtcccc actgaccagg gcgcatctga cagaggtaga atcaaggttg
      781 gaaaaattgg aagatttgtt tctgctcatg tttcctcggg aaaacctgga cagcattttg
      841 aacatggact ctttggacga agtgaaagta atgttgaaac aattatatat gcaggactat
      901 ggtgacacgg atgctgctat ggatagcttg acccctatgg aaaatggcat gtctgaaggg
      961 ccgagaaaac acatgaagac tgcgacatca tcgccaaaag tggatgaaga tggaagccaa
     1021 agtcagttga gtgtatcgcc ggaattcccg ctgcagtctg gtggcggagg ggctcctagg
     1081 ggttccggac gggctgacgc attggacgat tttgatctgg atatgctggg aagtgacgcc
     1141 ctcgatgatt ttgaccttga catgcttggt tcggatgccc ttgatgactt tgacctcgac
     1201 atgctcggca gtgacgccct tgatgatttc gacctggaca tgctgattaa ctaaggtacc
     1261 gaattcgcta gccggccata caagtaatcc ggatgatcga cgccaacgtc gttgaatttt
     1321 caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt
     1381 ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt
     1441 ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc
     1501 ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa
     1561 ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg
     1621 aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat
     1681 aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct tgggcccaaa aggccttttt
     1741 tttttggcgc cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1801 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1861 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1921 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1981 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2041 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2101 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2161 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2221 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2281 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2341 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2401 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2461 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2521 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2581 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2641 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2701 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2761 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2821 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2881 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2941 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3001 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3061 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3121 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3181 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3241 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3301 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3361 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3421 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3481 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3541 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3601 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3661 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3721 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3781 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3841 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     3901 agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt
     3961 ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta
     4021 acaacttgga aatgaaat
//
