LOCUS       sequence-390237-        4370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASE neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP012
FEATURES             Location/Qualifiers
     source          1..4370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1428..1577
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2115..2219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2220..2288,2289..3080)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2438..2457)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3251..3839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3740..3759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3993..4010
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4127..4148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4163..4168,4169..4186,4187..4193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4201..4217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4206..4228
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4225..4241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4225..4241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccagt tgaacatctc cctggtttct
       61 ccaatcaaca acgttcaagc tcccgccacc acggtctcca caaaaattgc gatttgattg
      121 attactacta gaataaatac attgaacatg gaattttttg aatacttttc tttttttaag
      181 tactggttct acccagaggt ggccggcgat tcccgttcgg caattttttg tcgacaaatt
      241 aggcaatttg ccggtttgcc gatttgccgg aaatgttcaa ttccggcaat tttccggttt
      301 gccaatttgc tgaaattttt taatttcgga aatttgccga gttgccgatt tgccgtaaat
      361 gttcaattcc ggcaattttc cggtttgccg atttgctgga aatttttaat ttcggaaatt
      421 tgtcgagttg ccgatttgcc ggaaatgttc aattccagcc ggtttgccga tttgctggaa
      481 atttttaatt tcggaaattt gtcgagttgc cgatttgtcg gaaaatttta attccggcat
      541 catatcagca gttttaacct ttcaatgccc cttgatgacg tgattcttta ctcagaacgg
      601 acttctgagc ttctgagcct cctgagccat gaaaaggatt ccttgaagag ctgctgacat
      661 aataatcatt atattattgc atatagccta ctctaagaac atgatgatta ataggaataa
      721 agttgaattt atggcttctt gtctcttttc ttcgctcttc ataagttgag cttatcattg
      781 ggcaagcttc ttttaggcgc gcctctagag gatccccggg attggccaaa ggacccaaag
      841 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagga
      901 tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg gaacaagctt
      961 gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag tgtgccaagt
     1021 gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc ccactgacca
     1081 gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg tttctgctca
     1141 tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac gaagtgaaag
     1201 taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct atggatagct
     1261 tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag actgcgacat
     1321 catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg ccggaattcc
     1381 cgctgcagtc tggtggcgga ggggctccta ggggttccgg acgggctgac gcattggacg
     1441 attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt gacatgcttg
     1501 gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc cttgatgatt
     1561 tcgacctgga catgctgatt aactaaggta ccgaattcgc tagccggcca tacaagtaat
     1621 ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt
     1681 ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt
     1741 caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc
     1801 tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg
     1861 ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac
     1921 aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat
     1981 atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga
     2041 attgtcctcg cttgggccca aaaggccttt tttttttggc gccgacgtca ggtggcactt
     2101 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2161 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2221 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2281 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2341 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2401 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2461 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2521 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2581 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2641 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2701 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2761 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2821 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2881 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     2941 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3001 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3061 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3121 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3181 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3361 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3481 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3541 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3601 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3661 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3721 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3781 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3841 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3901 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3961 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4021 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4081 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4141 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4201 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
     4261 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
     4321 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
