LOCUS       sequence-390238-        4826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASG neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP040
FEATURES             Location/Qualifiers
     source          1..4826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1884..2033
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2571..2675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2676..2744,2745..3536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2894..2913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3707..4295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4196..4215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4449..4466
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4583..4604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4619..4624,4625..4642,4643..4649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4657..4673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4662..4684
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4681..4697
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4681..4697
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccgtgc ttatcgtgtt aattggatcg
       61 agccatgagc aaggtaaggg ggctttttgg gagaattctt ttaaaaaaat caataatttt
      121 tttggaattt ttttgtcatt atttgtagaa ttttcagctg atagtcgata ggcttttgtt
      181 taaaataata gcattaaatt gctagaattt ctttttttta atttgtgcaa tttcaaaaca
      241 aagttttttt tgcccgaaaa aaagtcattt tgccactttt tggctgaaaa aaaattgggc
      301 aaaaataaaa aattgtctga atttgttgaa aacgggtaat tcatatatgg agaatttaga
      361 aaatctaggt ttaacccgtc gaaaactatt aaaaagtggc aaatatttgg caaaatgtgg
      421 cagaaagtgg cagaaaggca aaaagtggca gtgacaaaaa gtggcaaaaa aaagtgacaa
      481 aaaagtagca aaaatttatg gcaaaaattc acaattttga aactcctcta aaatggttaa
      541 ttttgtagtt aaaggactca aaattgatat tcgaactcta aaaaattgcc ctttttcaga
      601 atatagaggt tttgtacatt tccagaaatt tttttttgcc cgaaaaaaga caatttgaaa
      661 aaattggatg gctgaaaaaa aaacaaattg ggcaaaaata aaaagttgcc taagtttgtt
      721 gaaaacgggt aattcatata tggagaattc agaaaaacta tgcttaaccc atcaaaaact
      781 attaaaaagt ggcaaaaatt tcaaaatttt tttttttttt aattaaaaaa aaaatttttt
      841 gttcgacatt cgaaaactct cccaattatc agattacata tttttttacc accttaaatc
      901 accatttttt tcaggttttg gtggtccaat tgacgcaaaa tccaccaaat cgcaaacaac
      961 tacagctcaa actacagtac ccccaccgga aactacagct gcctcaaaag ttcatgcgac
     1021 cacttctgac acaaatcgaa tttcaatact catttctctt ttttctttat ctataatcca
     1081 actgtttccg tttttataat tgaattctca atgatataaa cctaaaccaa tttcaaagtt
     1141 cctggggtgg ggaattgaat gaaaataggc ggagtctgga gcacttgagc cctgaatcta
     1201 gtttcagaac aaatcagttt tcaaattttt ataactgtgt gttgactgaa aggcgcgcct
     1261 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     1321 aacatagaac attttcagga ggacccttgg ctaggatggg ccctaaaaag aagcgtaaag
     1381 ctagcatgaa gctgttgtct tcaatggaac aagcttgcga catatgccgg ttaaaaaaac
     1441 tcaggtgctc gaaggaaaag cccaagtgtg ccaagtgtct gaagaacaac tcggagtgcc
     1501 gttattctcc caaggctatg aggtccccac tgaccagggc gcatctgaca gaggtagaat
     1561 caaggttgga aaaattggaa gatttgtttc tgctcatgtt tcctcgggaa aacctggaca
     1621 gcattttgaa catggactct ttggacgaag tgaaagtaat gttgaaacaa ttatatatgc
     1681 aggactatgg tgacacggat gctgctatgg atagcttgac ccctatggaa aatggcatgt
     1741 ctgaagggcc gagaaaacac atgaagactg cgacatcatc gccaaaagtg gatgaagatg
     1801 gaagccaaag tcagttgagt gtatcgccgg aattcccgct gcagtctggt ggcggagggg
     1861 ctcctagggg ttccggacgg gctgacgcat tggacgattt tgatctggat atgctgggaa
     1921 gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg
     1981 acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaact
     2041 aaggtaccga attcgctagc cggccataca agtaatccgg atgatcgacg ccaacgtcgt
     2101 tgaattttca aattttaaat actgaatatt tgtttttttt cctattattt atttattctc
     2161 tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt
     2221 ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt
     2281 tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac
     2341 acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag
     2401 aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag
     2461 taaaaaataa gcaaagaaat taggcgaacg atgagaattg tcctcgcttg ggcccaaaag
     2521 gccttttttt tttggcgccg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3181 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3541 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3601 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3841 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4441 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4501 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4561 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4621 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4681 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
     4741 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     4801 atacgctaac aacttggaaa tgaaat
//
