LOCUS       sequence-390239-        6448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASI neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP017
FEATURES             Location/Qualifiers
     source          1..6448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3506..3655
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4193..4297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4298..4366,4367..5158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4516..4535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5329..5917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5818..5837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6071..6088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6205..6226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6241..6246,6247..6264,6265..6271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6279..6295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6284..6306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6303..6319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6303..6319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcaggccata cggactcaaa ttgaagcctt gtttgtataa gaaacgctga
       61 ttttttgatt ttttgttgat tttttaaaaa cgatgatcat tggaaatgcg gtttcccgtt
      121 ttcaaagatt tttcatcagt tttcaattgt ttttgttgtt gattttgaaa ttgctgccta
      181 ttctgttctg atttttcgaa aactaaaaat ctttgaaaaa tgaatgtaaa tttataattc
      241 tattttcgac cataaaattc agattctcta aatataatca ccgtcatcat gggataacta
      301 caatctgaca gaagacagag actcgagaaa atgtgaatac aaattggctg atatagattt
      361 tttcagaagc aagtagtcag tttctttgat tttaatatta tgatacccaa ttttgcgact
      421 ttcgatacgt aggtctctaa acgatcgaaa ttttacaact acagtacccc cgaatttttt
      481 tttgcgattt ttgttttcca aaaattcaaa gtccaagttc tacaaatgtt gaaatattaa
      541 gcaattttgg tgcaagccag gaatgggcgg caactgtcgt tcggcaaatt cgataatatt
      601 tgccggaaat tttccaattc cgagaaattg ccggtttgcc gatttgccgg aaatttttgt
      661 tttcggcaaa ttgctgattt gccgtttgtc ggatatcaga tttgtcagaa acgttgagag
      721 cgattgttta aagacggaaa ctcttaaact gtacctttta aaaatttttc atgttttctt
      781 tagatatttc atataatttc tacacttttc aaaatagatg taggaacatt cataggatgc
      841 gtacaatttt gccgattaaa attgaagttc tgaaattact tttaaaaaat gtacatattt
      901 tcggcaattt ccggtttccg acaaatcggc aatttatcgg tttgccgaaa tttttcaatt
      961 ccggcgattt gccgctttgt cgatttgccg gaaatttcaa ttccggcaat ttgccggaaa
     1021 tttcaattcc ggaaatctgc cgctttgtca atttgccgga aatttcaatc ccggcaattt
     1081 gccgatttgc cggaaacttc aattccggca atttgccgat ttgccggaaa cttcaattcc
     1141 ggcagtttgc cgatttgccg gaaaaaattc tacatttttt atattgcaat tttgtttgaa
     1201 ttattcgtgt taaaacattg taggagtcga aacatacgac attgctttgt atttcctcaa
     1261 aagctcatat gtttcaaaat tttgtaaatt tgccaaaact tttagatttt tcaacatttc
     1321 cggtcaaagt ttttggagtg ttttatttta atattcggtc gttttggatc tttataagtg
     1381 tatttaggca aaatctctac agacgctact tccaccttta attattgaaa caattcaaaa
     1441 gtgtcccaaa aaatcaccgt ccaaatgcct gcgtctctgc gcggagacac atcacaattc
     1501 aaatgatgtt tgtttttttt ttggcgccaa aacttctaga attaccaagt tagctatttc
     1561 aatttaatat tttgatttat tatcaattaa ttgaaccaag atttcagaat ttaaattttt
     1621 tttgtacttt ttggttaccg tatttcctct acaaatatat cctgacatac taataatttt
     1681 caaagtttga attcatttta tcaatgattt ttgaaaccca tgtcatttga aagatgacat
     1741 taaaaaaaaa taaacgcgcg gcaattgccg attaccggaa attttgaaaa cccgcaaatg
     1801 ccggaattgc cgattgccgg acacttccga ttgccgtgca cccctgctcc gaactagaaa
     1861 aagcctatct gaaactttca gtgcattttt ttcactttcg agttgctttt gaaaattgta
     1921 ttttatgaaa tatcatctga aacttttaaa acaatctgaa gtcagcgtca gcgctgacca
     1981 agtaacagag attttggctt tgcgagttag tacaagtcta taaatagtcg atctctattt
     2041 aaacgaggag aattttcaat ttcgaacacc ttaataagga gcgttttgac tatgtggtgg
     2101 gatagaaaaa agttgaacac atgtttgggc ttttaaatta cttaatttgt tccggcaaat
     2161 cacccctgtg agtctgcaaa tttttatttg aaactttagt actgaatact gaatactgaa
     2221 gtatacttaa gtactgaaag ttgtacacgt ggaaactttt tctgattatg taaaacattg
     2281 gttttgttat agaacagaac aatcatgtgc caatcgttga gtttattcta cttcaaatca
     2341 caagagtagt gataattagt aatcaaaaga ataataacta cgagatagtg tatgagttaa
     2401 cggagacaaa ggaaaggatc tcttgaggat cggttgggat ggagatgctc aacaacattc
     2461 acatatatac ctcgccatat ttctcatttt agtcatttat caactttttc tcctctctct
     2521 tttgttattg attagtcttt ctgatacgtg ttgtactgaa aaggttggga aacttctgga
     2581 acatccatta ccttgtggca actaaaggga aatagttatt taaaacagga aggtgatatt
     2641 gagaaggtgt ttgtttattc tttattttta aataaaaaaa aagaatcgga gataaattga
     2701 aaaaaaatca caaaaactaa ttatcagaag actaattatg ccacgtggtg ttttgcgaag
     2761 tgttagccta gaaactgttc cctttattcg tccttctcat tactctaaat taatttccct
     2821 catggttttt taagggtaga gccacttatt cattttgtga acacttttca acaggcgcgc
     2881 ctctagagga tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
     2941 ataacataga acattttcag gaggaccctt ggctaggatg ggccctaaaa agaagcgtaa
     3001 agctagcatg aagctgttgt cttcaatgga acaagcttgc gacatatgcc ggttaaaaaa
     3061 actcaggtgc tcgaaggaaa agcccaagtg tgccaagtgt ctgaagaaca actcggagtg
     3121 ccgttattct cccaaggcta tgaggtcccc actgaccagg gcgcatctga cagaggtaga
     3181 atcaaggttg gaaaaattgg aagatttgtt tctgctcatg tttcctcggg aaaacctgga
     3241 cagcattttg aacatggact ctttggacga agtgaaagta atgttgaaac aattatatat
     3301 gcaggactat ggtgacacgg atgctgctat ggatagcttg acccctatgg aaaatggcat
     3361 gtctgaaggg ccgagaaaac acatgaagac tgcgacatca tcgccaaaag tggatgaaga
     3421 tggaagccaa agtcagttga gtgtatcgcc ggaattcccg ctgcagtctg gtggcggagg
     3481 ggctcctagg ggttccggac gggctgacgc attggacgat tttgatctgg atatgctggg
     3541 aagtgacgcc ctcgatgatt ttgaccttga catgcttggt tcggatgccc ttgatgactt
     3601 tgacctcgac atgctcggca gtgacgccct tgatgatttc gacctggaca tgctgattaa
     3661 ctaaggtacc gaattcgcta gccggccata caagtaatcc ggatgatcga cgccaacgtc
     3721 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     3781 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     3841 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     3901 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     3961 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     4021 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact
     4081 agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct tgggcccaaa
     4141 aggccttttt tttttggcgc cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4501 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5281 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6061 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6121 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6181 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6241 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6301 cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta
     6361 actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat
     6421 ggatacgcta acaacttgga aatgaaat
//
