LOCUS       sequence-390240-        6617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASK neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP068
FEATURES             Location/Qualifiers
     source          1..6617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3675..3824
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4362..4466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4467..4535,4536..5327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4685..4704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5498..6086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5987..6006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6240..6257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6374..6395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6410..6415,6416..6433,6434..6440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6448..6464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6453..6475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6472..6488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6472..6488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgatgtatt taattgtgaa gcactctccc aaaaatatga gttttcttcg
       61 tgtcacatta gcactcacat ctgtgtctca attaattatt attgttttgg cgtgttttct
      121 aaaaatacgg tttgttacaa caaagaaccc tatggaaatg tggacgtatg gatattgtcg
      181 gtattttgag ccttggctat gctatactta ccaccaaatt gttcaggtaa aaagactatt
      241 attgatctag ttgattttta aaaaaagggt ttcagctgtg tcttctagct agtaacctta
      301 caatatacgg aacatttttt attaaataca tgatagtaaa atcaaagtca atgaataaaa
      361 cagtaatatt cagcacatat attgtatttt acttcccact tatattgtcc tcggtaagtg
      421 tgaaatctct ctaaaattga atacaacttg ataaattaca gatcattgtc gtgagtatta
      481 ttgaaaccca aacgtttaac tccgagtatg aagataaact aaattttgtg aattcattga
      541 gaattattaa cgcggattat cttatttttt cgtttttaag tttctcaaat tggcttaatt
      601 cttgcaattt gtttattctc gcgttttgca tgtttgctgc tccggctgtt tgttattact
      661 gtcgaaagta agttgggtca agacaaacgc catatttcaa caagtgttca acattcaagc
      721 aagtttatag accaatcatg gccagttttt tttttcagtt tcaaagcttt gtcgaaacat
      781 tctcaatcaa aaaatttaga taaatctcaa aaaaattcac ccaaaactaa ttgttcaagc
      841 agaaagaaca ttctagagca accgggaatt ttttaaatag gcatttttta tactgcgaac
      901 agaattttgc cgttttcttt taatgccata gtaaatgctc aggtgtgtac tacacacttc
      961 cgtaatttac ccacatcctc tactcttcaa tttaataatt ggaatgaaaa acctcttagg
     1021 cttttcagaa aaacgctaga tcatattaat ggtcacatcg caagtatttc acttgcacga
     1081 caatatcaac ataaaagttt tatcatggta actttttttt tggtatatgt agatttattc
     1141 aacttctgtt caggatctca ctattctatt aggttgagca aaaagtcttt gcaaaatttg
     1201 tacttttctt catttctcag cgttaattca tttttttctg attctgtttt tttatttgta
     1261 tagtacgtaa tgtgcatgtt taatgcattt gaaaaaactt ttgggtgctg ttcaaaatga
     1321 ccgagaattt gctcgccgaa cgccacgtcc tcctaggagt ttttctgtac gagttcctcc
     1381 aaggctgcaa ttgcaacgaa gctcgtcgta acatgtgtgc agtgttaggc aataactctg
     1441 tcacctacaa caccatgaag ttttggttcg aaaagttcac gaaaaagaac tacgatctcg
     1501 atgataaacc aagatcagat cgctctcgtt cggatatcga tgaggatatt tcgagagccc
     1561 tggaagatga ttcaagagca acgtcacgca aactttctgc gactctcaag catctccaaa
     1621 gaaccatcat caagcatctc catagaaccg gaagggtgcc aaagttcggt caacttgttc
     1681 ctcacaaatt gtccgattct cagaaaaatt gctttgtgac ctctctcttt cgctgttcac
     1741 taggaaacga acgacggact ggattaagga tatcattact ggagatgata aatgggtatt
     1801 atatgttagc cataccagga aaaaagagtg ggtcccggtc gaggaaaccg cgaaacctgg
     1861 catcaagtga gaacttcaag gaaaaaagtg cttctctcaa ttcggtggga cagtaagggt
     1921 gtcatttcca gagagcttct tccaaacttt gctacaatca acgctggcct atactgcatt
     1981 taattgaaaa aggtggtcca tgctcatcga ttacatcgcc ctagaggatc aaagttgttg
     2041 ctgctccatg accacgcaag accgcataca actttgaaga cccgccagaa gctccagacc
     2101 ctcagaatcc aattttcgtc tcacccaccg tattcaacgg acttgtctcc tactgactac
     2161 tatatgttcc gccctctcca aaatcacctt gccgggcaga agtttcatga tcgaaaggtc
     2221 gtcgaaactg ggttggacga cttctttgcc tcataatcgc aggagttcta ggcggagggt
     2281 actgttcaac ttccattgtg ttggcaagaa gtcataagca ttaatggtaa atatattacc
     2341 cattgaatct tgttcgcttt gaaaaaatac tacttccaaa aaaaaaatca aaattttgca
     2401 aagacttttt gctcaaccta atatgtatac ttccttgtgt gatgtatatt cctattgagc
     2461 ttttgtatta ctattgtatc agcacgaaag tggagatatt gtttctcgaa ttttcgttct
     2521 cgttaattgt tgcatttcct acaatgattg atcctctcat atctatatat tttgtcactc
     2581 cttatcgaaa gctggtgaca aaatgggttc aaaaagaatc agtgggaagt agagtaaata
     2641 caatgtaact ttgcttttgt ttctatgata aagccgtcca ttttgaagct aattttttgt
     2701 ttttataata attttagaaa ttgcgcgacc cgacccatct ctacccacct aataggcata
     2761 agcacaacat gacttaacgt ttcagctaat gacaatgatt tatacatggt tactctacct
     2821 gataatactt ttcatttagg aattcaaaac acaagttttc cgctgttcgc attgctgatg
     2881 tcctcattag tgaaaatatt tcttagattt cggttacatg ttactctcac ttcaataatt
     2941 cttgtttgta actcatgtca cgtatgacga caaaatctgc agaaactaca cgccctttgg
     3001 taaaaggata aagtactagt aatttttctt gtttcttcaa aaggcgcgcc tctagaggat
     3061 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
     3121 cattttcagg aggacccttg gctaggatgg gccctaaaaa gaagcgtaaa gctagcatga
     3181 agctgttgtc ttcaatggaa caagcttgcg acatatgccg gttaaaaaaa ctcaggtgct
     3241 cgaaggaaaa gcccaagtgt gccaagtgtc tgaagaacaa ctcggagtgc cgttattctc
     3301 ccaaggctat gaggtcccca ctgaccaggg cgcatctgac agaggtagaa tcaaggttgg
     3361 aaaaattgga agatttgttt ctgctcatgt ttcctcggga aaacctggac agcattttga
     3421 acatggactc tttggacgaa gtgaaagtaa tgttgaaaca attatatatg caggactatg
     3481 gtgacacgga tgctgctatg gatagcttga cccctatgga aaatggcatg tctgaagggc
     3541 cgagaaaaca catgaagact gcgacatcat cgccaaaagt ggatgaagat ggaagccaaa
     3601 gtcagttgag tgtatcgccg gaattcccgc tgcagtctgg tggcggaggg gctcctaggg
     3661 gttccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc
     3721 tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca
     3781 tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac taaggtaccg
     3841 aattcgctag ccggccatac aagtaatccg gatgatcgac gccaacgtcg ttgaattttc
     3901 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
     3961 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc
     4021 cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct
     4081 tctcgctttc atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag
     4141 gatgatggaa atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga
     4201 ggaaggttta aaagaaaaca taaaaatata tagaggagga aggaaaacta gtaaaaaata
     4261 agcaaagaaa ttaggcgaac gatgagaatt gtcctcgctt gggcccaaaa ggcctttttt
     4321 ttttggcgcc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5641 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6241 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6301 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6361 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6421 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6481 gctatgacca tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc
     6541 tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa
     6601 caacttggaa atgaaat
//
