LOCUS       sequence-390242-        4752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the AWA neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJL065
FEATURES             Location/Qualifiers
     source          1..4752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1810..1959
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2497..2601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2602..2670,2671..3462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2820..2839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3633..4221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4122..4141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4375..4392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4509..4530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4545..4550,4551..4568,4569..4575)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4583..4599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4588..4610
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4607..4623
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4607..4623
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactgta cttaaatata tagggacgtg cgaaatagca
       61 ttggaaaatg agaggacgca gagaaaaatt ttcagagcga cttttttcca gttcaaagtg
      121 ctcctctgcc gctacatttt ttggaggggc ctacttaaag aaggagtatc gtcaatggga
      181 aaattgtttt aaaatgtagt atttgtactc aaactttcaa ttatcgcaaa agaaaaacgg
      241 aaaaaatccg ttaacattca gcatttagcc gttgaaatcg acgaaagtgg ctgaaagtgg
      301 ctgaatttga atttcgcgcg caaatgaatc ctaggccacc acgcaacttt taggccatat
      361 tcgtaaacaa aatattgatt taaatgtaat ttttcgaact ttaattcgct tttctttctg
      421 gaaaagttca agaaaactgg gaagaagaaa ttgtattgag tattttttca aaggacaata
      481 ccaaaacaaa taaaaattcg aaagatcgtc gtggcctagg atttctgtag ttaattttta
      541 aagatttttt cgacttaaaa tgctgaatct aagcggattc tcccgttttt gttttgtaat
      601 attcttaagt ttgagcacga atactacatt ttaaaacaat tttcccattg acgatactcc
      661 ttctttaaag ggtcttcttc aaaatggcat aggtttcatt cagaagtccg ggattatgtt
      721 gaaatgcttc ccttgtgagt aggcagtaaa tacatccaat ttcacataat cgaagagaga
      781 tcattcattt aggatacatt gtgttgtgat tcatgacatt tactacaaca caaccagctg
      841 atgtgtgtga ttcaggtttt aattgtagca tcttgtgatt ggaataataa attcaatata
      901 ttttcccaat tctgaatttg tggatacaaa cgtattattt ttaactgaat acctcgtgaa
      961 atcagatttc agattttgaa aacaatatat ctgactataa gttgcgtatg tagacaaatt
     1021 ccaggaaaat aatggctcaa acgacacgcc cctggcttca agttactata aaataggtta
     1081 ggaacaaagc acatataacc atgtattcag tttaattaat tttttgttgg cggtggcatg
     1141 aaattctcat aatttcatta agatacctta cagctccggc gcgcctctag aggatccccg
     1201 ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt
     1261 tcaggaggac ccttggctag gatgggccct aaaaagaagc gtaaagctag catgaagctg
     1321 ttgtcttcaa tggaacaagc ttgcgacata tgccggttaa aaaaactcag gtgctcgaag
     1381 gaaaagccca agtgtgccaa gtgtctgaag aacaactcgg agtgccgtta ttctcccaag
     1441 gctatgaggt ccccactgac cagggcgcat ctgacagagg tagaatcaag gttggaaaaa
     1501 ttggaagatt tgtttctgct catgtttcct cgggaaaacc tggacagcat tttgaacatg
     1561 gactctttgg acgaagtgaa agtaatgttg aaacaattat atatgcagga ctatggtgac
     1621 acggatgctg ctatggatag cttgacccct atggaaaatg gcatgtctga agggccgaga
     1681 aaacacatga agactgcgac atcatcgcca aaagtggatg aagatggaag ccaaagtcag
     1741 ttgagtgtat cgccggaatt cccgctgcag tctggtggcg gaggggctcc taggggttcc
     1801 ggacgggctg acgcattgga cgattttgat ctggatatgc tgggaagtga cgccctcgat
     1861 gattttgacc ttgacatgct tggttcggat gcccttgatg actttgacct cgacatgctc
     1921 ggcagtgacg cccttgatga tttcgacctg gacatgctga ttaactaagg taccgaattc
     1981 gctagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt
     2041 ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt
     2101 cttgctttct aaaaaattaa ttcaatccaa atctaaacat ttttttttct ctttccgtct
     2161 cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg
     2221 ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga
     2281 tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat atggaggaag
     2341 gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa
     2401 agaaattagg cgaacgatga gaattgtcct cgcttgggcc caaaaggcct tttttttttg
     2461 gcgccgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2521 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2581 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2641 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2701 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2761 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2821 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2881 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2941 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3001 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3061 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3121 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3181 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3241 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3301 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3361 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3421 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4321 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4381 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4441 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4501 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4561 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4621 gaccatgatt acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt
     4681 taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac gctaacaact
     4741 tggaaatgaa at
//
