LOCUS       sequence-390243-        4268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the AWB neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP005
FEATURES             Location/Qualifiers
     source          1..4268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1326..1475
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2013..2117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2118..2186,2187..2978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2336..2355)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3149..3737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3638..3657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3891..3908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4025..4046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4061..4066,4067..4084,4085..4091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4099..4115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4104..4126
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4123..4139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4123..4139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccata gaaacaagat ttaacaagat
       61 tgtagattaa aaaaatgttg aaaattgaaa actactcaaa aaccatgtta tcagagtttt
      121 gttaatttat agcaacaatt ttcaagcagt gctcttaaaa acactatgaa aaatataaat
      181 catcgtgttc gtttttaaaa tttcacatca aggtattctt tatagacccg tatagtttca
      241 aaaggaaggt tggtttgact cttggcttga aaatagaaca attattttat agtagattca
      301 gaaaagacag ctgtccattg ctaggaaact gtgagatact tagatccaaa tgatgtattt
      361 acaggaaaca aataaggaac taaatagtac tccataaatc actagacatc attaatgaag
      421 cctattcaat tgtaatcata gattcctatt tcccaattga tagctatttt tgtgatcttt
      481 atcttatttt ttgaatgaca tctcagaccc tcaactttgt tcacaattca acaaaactcg
      541 tgaatagtca cctatttcca gtaactcaat tagttttcta ttatttgagg tctggtcaac
      601 aattttattt tatttctctc tctctttctc tctctagatc tagattttga gatttttcta
      661 aattcttaac acaaacttca tatcatttga ctaggcgcgc ctctagagga tccccgggat
      721 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      781 gaggaccctt ggctaggatg ggccctaaaa agaagcgtaa agctagcatg aagctgttgt
      841 cttcaatgga acaagcttgc gacatatgcc ggttaaaaaa actcaggtgc tcgaaggaaa
      901 agcccaagtg tgccaagtgt ctgaagaaca actcggagtg ccgttattct cccaaggcta
      961 tgaggtcccc actgaccagg gcgcatctga cagaggtaga atcaaggttg gaaaaattgg
     1021 aagatttgtt tctgctcatg tttcctcggg aaaacctgga cagcattttg aacatggact
     1081 ctttggacga agtgaaagta atgttgaaac aattatatat gcaggactat ggtgacacgg
     1141 atgctgctat ggatagcttg acccctatgg aaaatggcat gtctgaaggg ccgagaaaac
     1201 acatgaagac tgcgacatca tcgccaaaag tggatgaaga tggaagccaa agtcagttga
     1261 gtgtatcgcc ggaattcccg ctgcagtctg gtggcggagg ggctcctagg ggttccggac
     1321 gggctgacgc attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt
     1381 ttgaccttga catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca
     1441 gtgacgccct tgatgatttc gacctggaca tgctgattaa ctaaggtacc gaattcgcta
     1501 gccggccata caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     1561 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     1621 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     1681 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     1741 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     1801 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     1861 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa
     1921 attaggcgaa cgatgagaat tgtcctcgct tgggcccaaa aggccttttt tttttggcgc
     1981 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2041 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2101 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2161 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2221 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2281 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2341 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2401 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2461 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2521 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2581 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2641 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2701 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2761 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2821 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2881 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2941 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3001 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3061 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3541 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3781 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3841 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3901 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3961 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4021 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4081 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4141 atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa
     4201 ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga
     4261 aatgaaat
//
