LOCUS       sequence-390245-        4614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the BAG neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pDO068
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1672..1821
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2359..2463
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2464..2532,2533..3324)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2682..2701)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3495..4083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3984..4003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4237..4254
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4371..4392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4407..4412,4413..4430,4431..4437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4445..4461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4450..4472
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4469..4485
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4469..4485
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccacta tcctggtcat atcaactttc
       61 cagcagtaca attttaaact gaaaatagtt tccttacaat tatcagctcg ttttttcggt
      121 tcccgaaaaa aacatccgtg ttttaagata atgtgacgtc acacgcgacc ataatgcgag
      181 attgcatggc attgacgatc tggattaaag tacataccag tctatcaatt ttgttttaat
      241 taatgtttga tatttaattt aaaaacaaac attcaaacag attttgctag tttggctctg
      301 gtgtgttagc tgttatacta actagaatac atcgagctat taactttgac ctgcttaact
      361 tgtcatatga catgtttatc ataaagatgc atgtacgtca atgtttaggt tatcactatt
      421 cttatcacag gttcgtagaa gatcgattcc aactcccaca aatcaaacag actacatttc
      481 actacaaaac acgaaatagg ggctacaaat gtcgtttaaa ctactttaga cacctcaaat
      541 aaaacaaaaa taatctgccc catgccaggc tcgaactggc gaccttggca taacctatag
      601 actataagta ccacgcgcta accgactgcg ccaatggggc acggccctaa cgcatgagaa
      661 ttagccttga caagtatact gctctcctcg gtctcctaga ctctctccgt ctatgctgtg
      721 tctctgtgga ggggtaaggg atacacacat gaaccactgt gttgtgcggt ctcttgacca
      781 ttgccttaca tagaggaaaa ggtgggagac ctaaagagca acgagcgggg agagacgcag
      841 acatttgtat ttcaagggaa agggggagag gtgagctaat ggatattacg aatcgaagat
      901 gagattatta aaataggggg caacagtgta cagctaacgg aaactggcac ggaagttgga
      961 agatgttggt agtggaaaca ccttattcag attttagtca gcattttcaa acatcttcta
     1021 gcagaaatca acccgcaaag gcgcgcctct agaggatccc cgggattggc caaaggaccc
     1081 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct
     1141 aggatgggcc ctaaaaagaa gcgtaaagct agcatgaagc tgttgtcttc aatggaacaa
     1201 gcttgcgaca tatgccggtt aaaaaaactc aggtgctcga aggaaaagcc caagtgtgcc
     1261 aagtgtctga agaacaactc ggagtgccgt tattctccca aggctatgag gtccccactg
     1321 accagggcgc atctgacaga ggtagaatca aggttggaaa aattggaaga tttgtttctg
     1381 ctcatgtttc ctcgggaaaa cctggacagc attttgaaca tggactcttt ggacgaagtg
     1441 aaagtaatgt tgaaacaatt atatatgcag gactatggtg acacggatgc tgctatggat
     1501 agcttgaccc ctatggaaaa tggcatgtct gaagggccga gaaaacacat gaagactgcg
     1561 acatcatcgc caaaagtgga tgaagatgga agccaaagtc agttgagtgt atcgccggaa
     1621 ttcccgctgc agtctggtgg cggaggggct cctaggggtt ccggacgggc tgacgcattg
     1681 gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg
     1741 cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat
     1801 gatttcgacc tggacatgct gattaactaa ggtaccgaat tcgctagccg gccatacaag
     1861 taatccggat gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac tgaatatttg
     1921 ttttttttcc tattatttat ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt
     1981 aattcaatcc aaatctaaac attttttttt ctctttccgt ctcccaattc gtattccgct
     2041 cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac
     2101 gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac
     2161 acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa
     2221 aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta ggcgaacgat
     2281 gagaattgtc ctcgcttggg cccaaaaggc cttttttttt tggcgccgac gtcaggtggc
     2341 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2401 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2461 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2521 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2581 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2641 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2701 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2761 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2821 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2881 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2941 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3001 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3061 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3121 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3181 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3241 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3301 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3361 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3421 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3481 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3541 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3601 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3661 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3781 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3841 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3901 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3961 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4021 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4081 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4141 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4201 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4261 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4321 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     4381 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     4441 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     4501 ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa
     4561 aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
