LOCUS       sequence-390246-        7327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the IL1 neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP062
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4385..4534
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        5072..5176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5177..5245,5246..6037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5395..5414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6208..6796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6697..6716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6950..6967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7084..7105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7120..7125,7126..7143,7144..7150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7158..7174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7163..7185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7182..7198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7182..7198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccatg ttcacgtact ttatagttac
       61 cgtatacaca tattcttcat aatcctactt tattcatttt gcaattgagc aaagcatctg
      121 caggtgcctg atattttacg gcttccgccc attaccgtag ctgaaagagt ataaatagaa
      181 gattcatcgt tcaaatgttt aatgttcgac ttttcaaata atgtattcct actaatttct
      241 gaaaaataag ccatcaaatg cgtctagtat caaaatcatg taaaaactgt aaaacagatt
      301 ttgtatgaga cgcatttcga tgacgggcac aacagagaca ccgcccataa gacggtatct
      361 tttgatggta agaggtgtga atctataata cgactgtgtg atccatgtgt cggttaccat
      421 aaaaaggtac atctgtggtc ccaaaaaagg tgataatgag tattcaaagc attttagcag
      481 ctgcatagtt accgtatctt aataataata ataataattt attacgctcg ctgggagacg
      541 tgaaggaata cagaatacaa aagttatcat tgttcgcttg tactagaatt ggtgcagtcg
      601 ggcaattgtt tccattccat tagaatgttg tccttgtcct tgcgttgtcc ttgcgtagac
      661 tcccccatag tgggcgagcg attcccgagt gaaaataatc acggaatgaa aagagtatag
      721 atgttttcct actgtacagt gttttctcta tcagtgttgt cagtcattta cattaatatt
      781 atctttttca gagatgaaaa aggaaagcaa aactagatgt gaattttctg tgagtttgat
      841 aagcctttcc ataaaacgat cacaacattt gttgtacgtt ttgaagaaaa ttataaacta
      901 cgacatgcgg atacattatt ttcagacaca tttggcgttt ctatacggaa aactacaagt
      961 ttacatagct gcacctatgg cgacttatat ttgttttgat tttttggttt cggacttcaa
     1021 ataatattac ttttgttgat cacaaatact attcttcaac tttcaaaaaa ccgtccacaa
     1081 ttttttaaaa ttaaattttc atgtcagctt taacaacact ctttttcagt tctatttgac
     1141 ccttatttga cctttgcttt tatcaatttt tataatcata tttgcaaata aaatgattgc
     1201 caaggcaaat attttgtctg aaaaatatta aaagtcaaca aagttatgaa actttttcta
     1261 ccgaaaacta tcaaaaagtc tgagactgag acacggctat atttttattt ggctctcacg
     1321 attctggaaa agtacttttg tatgctgact actgtacttt ctcacataca aagatatata
     1381 tttacatttc tcgacatcat tccttttttt attctgtaag cattttccag aaagcttctg
     1441 atagtactca tcaaaacttg aaagatggtc ataaaagtca acagcggggg tctttatggc
     1501 gccgatgtat ttgaactgaa aacattcgga aaatatcgag gagaaatgaa caggaaaccc
     1561 gactttgagc aggtaacgtt ttttatattt tcataatgta ttacagtggt cacttttcta
     1621 taattttctc tatgtacagt tcatgacaaa aaatcaggtg ctggaggtga aaacaagtcg
     1681 attactctca atctaattta tctccgttcc tccttacatt tacccgaaaa tagtttctat
     1741 aggtcattgg gaaaaacatt acatttattt tttcaaagtt ctattcttat ttcaaaatgg
     1801 aggtttgtcc agtttatcct gattctgaaa atattccttc tccaatggtt cgaagtgctt
     1861 ctgatgttat tttaaactca gatccattta attctacaat ggaaaatttg gtaagcaaat
     1921 atttttagga tacattcaaa attgaaattt gaatttgaaa acatttgaca gtttctctaa
     1981 tataggtttc aatgaaagtt cttcactaca ccacaatgta ttagcgatca tgtcatacct
     2041 tacttttact tgacctagaa ttcagcaaaa aaatgcactg gagcactttc agatggctct
     2101 ttcaggggtt cagagttgag ttccgccgct aaaatttttg tggtgatcta gtctaaacat
     2161 aagctttcaa atgacatgcg tctcaattta aagtccgata ctagattgac ttcctttcct
     2221 tgttaggtta gctcaaaaat aactaattca cctttcctga cacttccccc tttaatgcat
     2281 ataaagcgcg tggtttttct tctcattctg ctccgacggg ctcataaaac cactgaattt
     2341 cccatcgcag aaaggaccac taaacaaaat tcagttttaa aaagtgagtg tccacctctc
     2401 attttgacca gtgaaaagcc atagcaaccc gtttttatgc aagtgggtca aaactaaaac
     2461 tacatcctcg tttctctttt tgcacataat tagacaaata cgcgataatt cagattaaaa
     2521 gaaagttgga gagaatatgg tggtaactaa ttagcaacct aacctagttt tttatctggt
     2581 gaaacaactt tcacgtttgt tcctaaagac tatttccgtt ttcatcactt ttagccgccc
     2641 gcgacaaatc caagaaaatc caaacaaagt gtcaatgttt tttgttgacg aaactatatc
     2701 aaatgtatgt tttttttaat acaaaaatat atgtttctat gattctaagc tttcattttt
     2761 attgtagcat gtacacaaat taaaatattg aatataattt ttgttaactg aacttttttc
     2821 ttttcattgt agttcttaaa gttggaaaaa gtcagctaat aaatttttgt cacgtacgag
     2881 cttcaagcta gatcatttaa gtagattcta aatcaccagt aatcacaatt ttttaatttg
     2941 aagcacttta tttttcccat ctgatattcg ataacttttc aaatgtacaa aatatctgaa
     3001 tttttaaaaa atcaagttta aaatggtttt attagatttt tggttttcaa aacgctaaaa
     3061 aattcagaac atggaatcaa agttctagaa ccataacaag cgtggttcaa aaagtgttta
     3121 actttctttg caaaaacact tcgcggtggt cactcttgat ttctcttcta gccatcttca
     3181 aaaatgaaaa ttgaaaaatc gaacgaccgg tataaaatgg taggaggtgt cttcaaatcg
     3241 tcttcttcaa tccattcatg acgttctcat catttatgac ttccaaagtt ttagacgtat
     3301 tccatacaca ctcgcttttt cattttataa ttcataagta aaaagaacta acaaatcgtt
     3361 tctattttct gttttctttt caaaaaccac acgagttgtg gttctttttt gagaaccaaa
     3421 agaaaaatta ccggaaacgt ctatttcacg gtgaattggg cttcaagtaa gaagcattga
     3481 gcaattttta cctaaagttg ttttgaaata cgcctatttt taccaaaaca ttgtggtttt
     3541 tggttaaaat ttcaaaaccg acaacttttt ttcccagatc ccgtgatctg ttctcgtcaa
     3601 ttttccatca aaaaagtgtt tgagatctgg ataattggac ggactttttc atatgcaccc
     3661 ccctccccat ggatacttat tctcttgtca aactttattt tcattagtta ctttttattt
     3721 ttcagaaaaa cagttcagat attgttccga aaggcgcgcc tctagaggat ccccgggatt
     3781 ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     3841 aggacccttg gctaggatgg gccctaaaaa gaagcgtaaa gctagcatga agctgttgtc
     3901 ttcaatggaa caagcttgcg acatatgccg gttaaaaaaa ctcaggtgct cgaaggaaaa
     3961 gcccaagtgt gccaagtgtc tgaagaacaa ctcggagtgc cgttattctc ccaaggctat
     4021 gaggtcccca ctgaccaggg cgcatctgac agaggtagaa tcaaggttgg aaaaattgga
     4081 agatttgttt ctgctcatgt ttcctcggga aaacctggac agcattttga acatggactc
     4141 tttggacgaa gtgaaagtaa tgttgaaaca attatatatg caggactatg gtgacacgga
     4201 tgctgctatg gatagcttga cccctatgga aaatggcatg tctgaagggc cgagaaaaca
     4261 catgaagact gcgacatcat cgccaaaagt ggatgaagat ggaagccaaa gtcagttgag
     4321 tgtatcgccg gaattcccgc tgcagtctgg tggcggaggg gctcctaggg gttccggacg
     4381 ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt
     4441 tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag
     4501 tgacgccctt gatgatttcg acctggacat gctgattaac taaggtaccg aattcgctag
     4561 ccggccatac aagtaatccg gatgatcgac gccaacgtcg ttgaattttc aaattttaaa
     4621 tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt tttttcttgc
     4681 tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc cgtctcccaa
     4741 ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct tctcgctttc
     4801 atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag gatgatggaa
     4861 atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga ggaaggttta
     4921 aaagaaaaca taaaaatata tagaggagga aggaaaacta gtaaaaaata agcaaagaaa
     4981 ttaggcgaac gatgagaatt gtcctcgctt gggcccaaaa ggcctttttt ttttggcgcc
     5041 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5101 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5161 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5221 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5281 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5341 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5401 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5461 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5521 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5581 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5641 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5701 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5761 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5821 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5881 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5941 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6001 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6061 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6121 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6841 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6901 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6961 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7021 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7081 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7141 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7201 tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat
     7261 tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa caacttggaa
     7321 atgaaat
//
