LOCUS       sequence-390248-        5243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inhibition of synaptic vessel release due to disruption of synaptic 
            vessel function by reactive oxygen species production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pDO001
FEATURES             Location/Qualifiers
     source          1..5243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2988..3092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3093..3161,3162..3953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3311..3330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4124..4712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4613..4632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4866..4883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5000..5021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5036..5041,5042..5059,5060..5066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5074..5090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5079..5101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5098..5114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5098..5114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 gagaaaagtt tcgtgataac tgatccacgg ctgccagaca atcccatcat cttcgcatcc
      901 gatggcttcc tggagctgac cgagtattcc agagaggaga tcctgggccg caatggccgc
      961 tttctgcagg gaccagagac agaccaggcc acagtgcaga agattcgcga tgccattaga
     1021 gatcagcgcg agattaccgt gcagctgata aactacacaa aaagcgggaa gaaattctgg
     1081 aacctcctgc acctccagcc catgagggac cagaagggtg agctccagta tttcatcgga
     1141 gtgctgctgg atggatcagg aggaggctca ggaggaggag gcgcgcctac cggtatgtcg
     1201 gctaccgctg ccaccgtccc gcctgccgcc ccggccggcg agggtggccc ccctgcacct
     1261 cctccaaacc ttactagtaa caggagactg cagcagaccc aggcccaggt ggatgaggtg
     1321 gtggacatca tgagggtgaa tgtggacaag gtcctggagc gggaccagaa gttgtcggag
     1381 ctggatgacc gtgcagatgc cctccaggca ggggcctccc agtttgaaac aagtgcagcc
     1441 aagctcaagc gcaaatactg gtggaaaaac ctcaagatga tgatcatctt gggagtgatc
     1501 tgcgccatca tcctcatcat catcatcgtt tacttcagca ctagcggcgg aagcggcggg
     1561 ggaatcgatg gaccggtaga aaaaatggga tccatggtct caaagggtga agaagataac
     1621 atggcaatta ttaaagagtt tatgcgtttc aaggtgcata tggagggatc tgtcaatggg
     1681 catgagtttg aaattgaagg tgaaggagaa ggccgaccat atgagggaac acaaaccgca
     1741 aaactaaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     1801 gtaactaaag gcggaccatt accattcgcc tgggacatcc tctctccaca gttcatgtat
     1861 ggaagtaaag cttatgttaa acatccggca gatataccag attatttgaa actttcattc
     1921 ccggagggtt ttaagtggga acgcgtaatg aattttgaag acggaggagt tgttacagtg
     1981 acgcaagact caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     2041 ttcagcctcc aagatggaga atttatttat aaagtcaaac ttcgaggaac gaatttcccc
     2101 tcggatggac ctgttatgca gaagaagact atgggatggg aagcttcaag tgaaagaatg
     2161 taccctgaag acggtgctct taagggagag attaaacaac gtcttaaatt gaaagatgga
     2221 ggacattacg atgctgaggt aagtttaaac atgattttac taactaacta atctgattta
     2281 aattttcagg tgaagacaac ttacaaagcc aaaaaaccag ttcagctgcc aggagcgtac
     2341 aatgttaata ttaaactgga tatcacctcc cacaacgagg attacactat cgttgagcaa
     2401 tatgaaagag ctgaagggcg gcactcgaca ggtggcatgg atgaattgta taagtagcat
     2461 tcgtagaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     2521 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     2581 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     2641 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     2701 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     2761 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     2821 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     2881 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc
     2941 tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3001 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3061 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4921 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4981 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5041 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5101 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     5161 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     5221 cgctaacaac ttggaaatga aat
//
