LOCUS       sequence-390249-        5615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the URY neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP085
FEATURES             Location/Qualifiers
     source          1..5615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2673..2822
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3360..3464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3465..3533,3534..4325)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3683..3702)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4496..5084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4985..5004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5238..5255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5372..5393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5408..5413,5414..5431,5432..5438)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5446..5462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5451..5473
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5470..5486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5470..5486
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccgttc caattggtcg taaaagtgct
       61 gcatacgctc tgatgggtgc catgttcacg tcttccggaa atgaaaagaa gcaagttcaa
      121 attatgacgg aagtgaattc acggcttcag gcggttctag aagatgttat tcagaagaat
      181 atattaatga gggtgaggga aatttcctga aaattttgag aaaccagaga atttctagaa
      241 aaactctgga aaaagacaaa aaaaatctgg gaattataga aaacttttag aaacttctgg
      301 aaactttcaa aaattctgga aagttcaaaa aacgtttgcg aaccttgagg aatttctgga
      361 aacatctgga aagttttgac aaaattctag gagttttagg aaacttctgg gaacgttaaa
      421 atattctggg aattatggaa aacttccggg aactttttag agaatttgga aaacttttgg
      481 aaaattctgg aaattttttg gaattcacga gaaactgtga tagagtcttg gaagtttcag
      541 gaaacttctg gaaagtctta gcaaaattct agaagtatta gaaattttag gaaacttttg
      601 gaaagttctg gaaagttgta ggaaactttt ttaaagcttt ctctgacatt ctagatactt
      661 ctggaaagtt aaaaacaaat tctatgattt ttttttgaaa actttctagg aaatttggaa
      721 attttctgaa aaattcaaaa aagttctgag aaacttttgg aaagttcgga aagttaacaa
      781 aattctagaa atttcagaaa attaaaggat taaacgggaa ctttccagag aatttggaaa
      841 acttctggaa aattctggga attttgggaa attttttaaa agtttcctgt aatctatcta
      901 gaaagtcctg aagagttaga aaaaagttca aggaattttt tttgaaaact ttctagaaaa
      961 ggtggaaatt ttctgaaaaa ttcaagatct agaaattcta agaaattttt ggaaaattct
     1021 ggaaaagttt ttaaaaaaat ctaggaattt ctgaaaactt ctggaaagct agtagaaact
     1081 tctaaaaacg ttcaaaaact ctggaacatt tttttaaaaa ttctaggaag tttaggaaac
     1141 ttctagaact ttctagaaac tcctggaaag ttaaaaaata attctcggaa ttttaggaaa
     1201 attccgggaa ttttctagag aatttgggaa actggtggag agttctggaa agtttaaaaa
     1261 agtttaggaa ccttgagaaa tttctagaaa cttctggaaa gctaagaaat tctggaaatt
     1321 ctacgaaact tccggggaat ttcttgaaaa ctttctagaa ccttctgaaa aaattttaaa
     1381 aaaattctag taatttagga aactcccaga aaattcttga gaactttcta ggaaatttgg
     1441 aaattttctg aaaaattcga gaaagttctg agaaactttt ggaaattact agaaagctct
     1501 gtaacgttcg gtaagctttt ctgaaacttg agaaacttct agacactttg agagatttct
     1561 ggaaatttct ggaaatttct gaaaattttt aggaaaatta aatctgagct tgaagtgtgc
     1621 aaaaaactga atattattgt ttataaaaca taaattcaaa aagtataaat tttcttaatt
     1681 ttcagagctc cgtggacact ttatcagctg ataacacccg tttatcacgt gaaaaccgac
     1741 tgctaagcct atcgcaagtt cgaacaactc aggataatta attgcaaaaa ttaaaattga
     1801 acactttatt tttgtgcctt tctctattca ttctccccat tttttcatca tcatcatatc
     1861 ccaaaagttt tccgccaaaa taaagtgact aaacccataa accctgccac ctgggaccct
     1921 ttgttagctc cgcctacatt tccattttca aattagggac cgcctgaatt cacatttcag
     1981 tgctcaaaga gctttttctg gtctgccagt tttctacgga attttacgaa tttttgaaaa
     2041 ggcgcgcctc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga
     2101 tactaacata acatagaaca ttttcaggag gacccttggc taggatgggc cctaaaaaga
     2161 agcgtaaagc tagcatgaag ctgttgtctt caatggaaca agcttgcgac atatgccggt
     2221 taaaaaaact caggtgctcg aaggaaaagc ccaagtgtgc caagtgtctg aagaacaact
     2281 cggagtgccg ttattctccc aaggctatga ggtccccact gaccagggcg catctgacag
     2341 aggtagaatc aaggttggaa aaattggaag atttgtttct gctcatgttt cctcgggaaa
     2401 acctggacag cattttgaac atggactctt tggacgaagt gaaagtaatg ttgaaacaat
     2461 tatatatgca ggactatggt gacacggatg ctgctatgga tagcttgacc cctatggaaa
     2521 atggcatgtc tgaagggccg agaaaacaca tgaagactgc gacatcatcg ccaaaagtgg
     2581 atgaagatgg aagccaaagt cagttgagtg tatcgccgga attcccgctg cagtctggtg
     2641 gcggaggggc tcctaggggt tccggacggg ctgacgcatt ggacgatttt gatctggata
     2701 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
     2761 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
     2821 tgattaacta aggtaccgaa ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc
     2881 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
     2941 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
     3001 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     3061 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     3121 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     3181 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     3241 gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg
     3301 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     3361 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3421 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3481 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3541 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3601 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3661 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3721 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3781 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3841 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3901 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3961 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4021 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4081 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4141 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4201 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4261 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4321 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4381 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4621 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5281 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5341 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5401 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5461 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     5521 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     5581 caacgatgga tacgctaaca acttggaaat gaaat
//
