LOCUS       sequence-390251-        5060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the PHC neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP173
FEATURES             Location/Qualifiers
     source          1..5060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2118..2267
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2805..2909
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2910..2978,2979..3770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3128..3147)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3941..4529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4430..4449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4683..4700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4817..4838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4853..4858,4859..4876,4877..4883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4891..4907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4896..4918
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4915..4931
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4915..4931
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcaagaat tcttatcgga tgcgatagag aattttttta atattatgga
       61 tacgtttctg ggtctagaaa ttcgattttg gccgcgctag aggcgttttg gtcttaaatt
      121 cattttttac atgcatatat gactgcactt ttctgcaaaa cttgttggtc cagaaactta
      181 tccataatat ttaaaaaaaa gcagattttt tcaaaaattc aaaagtatgg gaaaatcata
      241 tggagtcatt cttttttatt tcataaaact gttcagcata gtcaaaaata ccccagaata
      301 tgccaaaaaa agtatactag cttgtacgaa agtatttttt taaaaattga taaaaatata
      361 taaaagctga ttttttcaaa aattcaaaag tatgggaaaa tcatatggag tcattctttt
      421 ttgcgtactc atgattctgt aactataata cactttaatt tgtgaaaagt tgaaaatatt
      481 atgtatgtga gaaactatac gtcgccgact aaaaaagaaa ggaatgaaat ttgggatgag
      541 gaagaaaact acttgttttt gttttgtacc gattgcgtaa tatattttcg cgagtggtaa
      601 acattgacgc actgttcaag taatcagaag caaggtaatt gtttattatg tatgacctca
      661 atttaaaaaa aaataaataa aaatgaatca aggaaatcgc tagacttatt tttcaggtct
      721 attcaaactt gataagttaa acgaaaaaat tgtaaaaaaa aatcattgta ttttttaagt
      781 gtggggttcc aatatagaag cactaagaat atatgcaacg tcaattttaa atcaattttt
      841 gtaaggataa cactagataa atatttaaaa aaggcgagat aaaactagca acgtgttgcg
      901 aagggttgtt ccactgctgt tcaattgcta atagttagtt gtgagagagg aaaatgattg
      961 caaaatcata tgaccgcggc ggaaaactgg caaaacactg gcaatttttt gggatgagga
     1021 ataaatgagg aacaaacaat ttatgaaata cttcaaagaa aaaacatgaa aaccgatgaa
     1081 caaggtgaag cgactttctc gcacggcata attttctgtt ggcgttctta ttttgaaaaa
     1141 aggctaaaaa agtattggtt tgagaaacat gaaaactaaa ttgatatctt ttcaagtttt
     1201 tcactaaagc gtttcgaaat taacgctgga cgtgcttttg ccatgaatta gatcagtgta
     1261 atacaaacaa tttggtatct tactgataga agtaaaatca gattttatat agttcacagt
     1321 cagtggtgca gatgccttct cagcaactcc aagtaacatc tctcccatca tcagagtaag
     1381 catcaagttc ataatgattt tttttcagca ttacatatgg tgtttctgtc acacgaaaat
     1441 gtggtttgag cgggatactt gaacatttct ttgtaactga gctttttagt cttttgttaa
     1501 catgcacatt tgtggccggc tccgagaaac caatctggat atgaaatttt tataggtgtg
     1561 agcaataaac acgcgttgtt cgaatattaa aatgtgccat attacatgct caaaattcag
     1621 taaaccacac cctatggcca tgcgtatcta aagttaacaa catcaacttt taacatttct
     1681 tgtattttca gctgtctctc ctttttaacc acggcgtcac aggaaaattc ttgaatttcc
     1741 aaaaatatat aatttccttc aagtttcctc ttggacgtaa tttgaaaaat tcaataaaag
     1801 tgcattttca atggcgcgcc tctagaggat ccccgggatt ggccaaagga cccaaaggta
     1861 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctaggatgg
     1921 gccctaaaaa gaagcgtaaa gctagcatga tgttgaagaa gattctcaaa attgaagaac
     1981 tggatgagcg tgagctcatc gacatcgagg tctccggaaa ccacctcttc tacgccaacg
     2041 acatcctcac ccacaactcc tcctccgatg tactgcagtc tggtggcgga ggggctccta
     2101 ggggttccgg acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg
     2161 ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac tttgacctcg
     2221 acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt aactaaggta
     2281 ccgaattcgc tagccggcca tacaagtaat ccggatgatc gacgccaacg tcgttgaatt
     2341 ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg
     2401 ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct
     2461 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     2521 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     2581 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     2641 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa ctagtaaaaa
     2701 ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccca aaaggccttt
     2761 tttttttggc gccgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2821 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2881 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2941 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3001 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3061 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3121 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3181 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3241 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3301 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3361 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3421 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3481 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3541 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3601 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3661 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3721 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3781 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3841 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3901 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3961 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4021 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4081 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4141 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4201 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4261 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4321 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4381 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4441 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4501 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4561 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4621 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4681 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4741 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4801 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4861 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4921 acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta
     4981 ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc
     5041 taacaacttg gaaatgaaat
//
