LOCUS       sequence-390252-        5655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the PHB neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP026
FEATURES             Location/Qualifiers
     source          1..5655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2713..2862
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3400..3504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3505..3573,3574..4365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3723..3742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4536..5124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5025..5044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5278..5295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5412..5433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5448..5453,5454..5471,5472..5478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5486..5502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5491..5513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5510..5526
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5510..5526
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccccac agttactgtt gttacagact
       61 ctacatctat taatataatg cggtcactct acatcatcag cctgacaatc ctcgacatag
      121 tactttgcat ggctccaatg cctcatattg attggtgtct ttattggaga tatttcaata
      181 aggatcaatt taggcccgaa tgtaacggag tgtaccgtct gaggtttgcg aaacataaag
      241 gtgaggaaaa aagtttttat taacttttta aagaaaattg ataatcaatt ctttcattca
      301 ttcttctcat tcagcataat tcctaattcc agctcacgat catcgacccc gagtaacccc
      361 tcaactatca agtgtcccat ggacaccagc agcaccagtt ccaagattca actcttttgt
      421 caatacgaac tctataatca taagcaatga aaatggattt ggtaagagtt gaaagttaaa
      481 ttaaagccag aatgaaattt ctaagttgcc atcttaaaat caaatgttaa tgcatttcag
      541 gagagtgtcg accgcatcat acaaattgcc agcaatccgg acaatgttca tcaaatcaat
      601 tatgcataga cagtaatgga tattgttgtg gcaataaaaa gtaggttaag caattgattt
      661 tgaaatcaca taatttccaa tttatttttt tcctaacgaa acttagtttt ttttaatgag
      721 aatacaaaaa tagaaatcga aatttccaga ataataaccg aatgcccatc accatcctct
      781 cttgttgctc attgtgcatt ccgtcgtcgt caagtgaatt ggtgtgaatc agattccgaa
      841 tgtgcaccac acttggctcc acgtcagaac agatttttct ttaacgacgc cacagttctg
      901 gacagcattc agaagtctcc atcactttgc tgcccgaccc aatgtggtta taatgtgtgt
      961 gttcgatagg cttctggtat tgtgctcttt atacttgaaa gagcacaaca cacctgtgac
     1021 cagagtcctc atgacaagaa ttttttttca gtatttgtat atttcgatga gaaaaaaaca
     1081 cactttttgt atttttagtt tggtcagatc tttgccaaaa ctgctgaact tttgagcaaa
     1141 actatttgac gcatgacttt cattctttgc gattgatgtg ctatgcagat tttcttgaaa
     1201 ctttagcata ccgaaatttt attaattaaa agcaattcgg tcttttcgaa ataatttttt
     1261 taaacctcga ttaatatata ttacttcctt ttctccccct ttttatgtat gttttatgat
     1321 tgtgatttcc aatacgtgta cattcttgat taatttattc tctccagcaa caagttgcaa
     1381 attggaatta gagagtcagt gttagagaaa tacgagtttc cctcttttta atgtaaagtt
     1441 tattgtttca ccactcgtgc aggtgttaac atgctttttc gatcgccctt acgaactttt
     1501 ttatttatat gtcaataaat attttactga tttcataaaa ttatttttaa tcatttattt
     1561 actattttaa aaaaaattaa aatgttgcat gattttaata accaaaatta ctaattttaa
     1621 aaagaaacaa cactggtttt atgttgtctg atctgagaaa ttcgttagtg aaatacaaga
     1681 aaaatagcaa aaataactat gtatgtacaa aataataggg caccaacaaa aatatttttc
     1741 tgtaatatat ttgtcatggt tttctggatt ttaaaacact caaaatatta tcttgaatga
     1801 gcaaggcata gcttaatcta gagaaaatga tgtgagctga tgtgaacaga aaaaagatac
     1861 gttccaaaaa gtcaccaatt ttcattgtat tatttaccag cattaatttt tttaaatcaa
     1921 ggtagagtag cgtcagctag gaaattgtta aaacctggat aaaattgcca gttattataa
     1981 aaagcatttc aaaaataatt ttaaattttc taatagtagt caaaaattgg tggttattca
     2041 gttttgataa ttcgtaattt aggaaaacta ccgttttttt tttcaaaatt ttacaagaag
     2101 cttttgacta gaaattttaa aataactgaa atgttttgga gggaatcagt atgaaatttg
     2161 gtatttttgg attattttat atctatttag ataaaattgg tgccgtttct ttgactattt
     2221 gttgttcaaa agaccagcag accaaaagct aagcttaaaa tttctgcata tatttcagtt
     2281 cttttttaca tcgtaaaaga aaaaattcta atctttttac ctccattgcg acattttccc
     2341 agactttatg catcaaacat acctcctttt ttgttttcta gaaactactg aaccggatat
     2401 atgccagggc gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt
     2461 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag gatgggccct
     2521 aaaaagaagc gtaaagctag catgatgttg aagaagattc tcaaaattga agaactggat
     2581 gagcgtgagc tcatcgacat cgaggtctcc ggaaaccacc tcttctacgc caacgacatc
     2641 ctcacccaca actcctcctc cgatgtactg cagtctggtg gcggaggggc tcctaggggt
     2701 tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc
     2761 gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg
     2821 ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaacta aggtaccgaa
     2881 ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa
     2941 attttaaata ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt
     3001 tttcttgctt tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg
     3061 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc
     3121 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga
     3181 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg
     3241 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag
     3301 caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gcccaaaagg cctttttttt
     3361 ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5341 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5401 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5461 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5521 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
     5581 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
     5641 acttggaaat gaaat
//
