LOCUS       sequence-390253-        4593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the PHB neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP063
FEATURES             Location/Qualifiers
     source          1..4593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2338..2442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2443..2511,2512..3303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2661..2680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3474..4062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3963..3982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4216..4233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4350..4371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4386..4391,4392..4409,4410..4416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4424..4440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4429..4451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4448..4464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4448..4464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccttg acaccgactg gcacacacaa
       61 aatattaaac tttcagggtc taatccatca cgaaatgaac gaccacaacc tcaatgcaaa
      121 atgtcattta tgccagaaat ctttcgagaa taagaagaca ttccgtgaac actgtgaatt
      181 ggatcacaca ccggaagtgg tatgacagta ttcattaaca gattcaattt gaaatattaa
      241 ttttaggtta gatgtgtatt ctgcaaaagc tcattcgatc agccactgga aatgaacgat
      301 gaacaatggg cacacttttt cgctcatatt tatggggtta gtaattcacc attcgaatct
      361 agaagtcgat tctcctgact gacattttta aaaccatttt ttccaggaaa tcctctactc
      421 ccgccttgcc gactacgagg atcgtgcttt gaacactgca tgaattatat atttgattcg
      481 atggttagct taataaagaa tttttctgtt gtacttcaac ctttttctga aataggaaat
      541 aatcacaagt tgtctgaaaa atgtgatcag ctatccagat caaacagtga aaatgttgtc
      601 tgcaatgatt catagctgaa catctgaact cttaaatttt cagcgaatca tgatttgcat
      661 tgtgatatat tattcttatg gttgcaaaag aaaaaagatt acgaaaatat cttgtgacag
      721 agtaagaaac cattgaaatt taattaatca ccgtggagac aggtgtgcac tctgctctta
      781 ttatattatc aaaacgagaa acgcgtgagc aagaagtggg cgtgacttgg tagaaaggta
      841 cttaatcgag atgttgagcc aactttcgag attattctaa aagtttccat atcaataaac
      901 ttttttggaa atttcatctt gcaagggcgc gcctctagag gatccccggg attggccaaa
      961 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc
     1021 ttggctagga tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg
     1081 gaacaagctt gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag
     1141 tgtgccaagt gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc
     1201 ccactgacca gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg
     1261 tttctgctca tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac
     1321 gaagtgaaag taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct
     1381 atggatagct tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag
     1441 actgcgacat catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg
     1501 ccggaattcc cgctgcagtc tggtggcgga ggggctccta ggtgcctcga cctcaagacc
     1561 caagtccaaa ccccacaagg aatgaaggag atctccaaca tccaagtcgg agacctcgtc
     1621 ctctccaaca ccggatacaa cgaggtcctc aacgtcttcc caaagtccaa gaagaagtcc
     1681 tacaagatca ccctcgagga cggaaaggag atcatctgct ccgaggagca cctcttccca
     1741 acccaaaccg gagagatgaa catctccgga ggactcaagg agggaatgtg cctctacgtc
     1801 aaggagtaag gtaccgaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca
     1861 acgtcgttga attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt
     1921 tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca
     1981 tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt
     2041 gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg
     2101 gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc
     2161 aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga
     2221 aaactagtaa aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc
     2281 ccaaaaggcc tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc
     2341 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2401 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2461 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2521 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2581 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2641 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2701 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2761 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2821 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2881 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2941 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3001 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3061 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3121 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3181 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3241 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3301 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3361 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3421 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3481 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3541 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3601 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3661 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3721 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3781 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3841 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3901 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3961 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4021 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4081 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4141 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4201 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4261 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4321 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4381 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4441 tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct
     4501 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
     4561 acgatggata cgctaacaac ttggaaatga aat
//
