LOCUS       sequence-390296-       12838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized SpRY and blasticidin resistance: EFS
            promoter-SpRY-NLS-FLAG-P2A-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH
  TITLE     Deep learning models to predict the editing efficiencies and 
            outcomes of diverse base editors.
  JOURNAL   Nat Biotechnol. 2024 Mar;42(3):484-497. doi: 
            10.1038/s41587-023-01792-x
  PUBMED    37188916
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2024-03"; volume: "42"; issue: "3"; pages: 
            "484-497"
COMMENT     Sequence Label: SpRY
FEATURES             Location/Qualifiers
     source          1..12838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        530..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             746..926
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    973..1098
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1591..1824
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2009..2053
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2351..2468
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2561..2772
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2791..2800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2797..6900
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6901..6948
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6949..6972
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6982..7038
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7039..7437
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7462..8050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7515..7535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7933..7944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8053..8069)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8054..8070)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8575..8755
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8781..8798)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8787..9011
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9057..9485
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9144..9163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9354..9375
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9494..9514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9659..9793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9720..9739
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9855..9902
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9921..10295
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10425..10546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10462..10481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10516..10535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10595..10611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10595..10611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10608..10630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10619..10635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10643..10649,10650..10667,10668..10673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10688..10709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10826..10843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10997..11585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11077..11096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11756..12547,12548..12616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12379..12398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12617..12721)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12796..12815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
       61 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      901 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      961 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     1021 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     1081 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1141 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1201 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1261 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1321 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1381 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1441 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1501 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1561 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1621 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1681 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1741 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1801 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1861 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1921 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1981 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     2041 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     2101 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2161 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2221 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2281 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     2341 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     2401 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2461 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt agctagctag
     2521 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     2581 acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg
     2641 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     2701 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     2761 gccagaacac aggaccggtt ctagagcgct gccaccatgg acaagaagta cagcatcggc
     2821 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     2881 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     2941 ggagccctgc tgttcgacag cggcgaaaca gccgagcgga cccggctgaa gagaaccgcc
     3001 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     3061 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     3121 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     3181 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     3241 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     3301 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     3361 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     3421 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     3481 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     3541 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     3601 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     3661 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     3721 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     3781 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     3841 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     3901 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     3961 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     4021 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     4081 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     4141 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     4201 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     4261 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     4321 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     4381 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     4441 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     4501 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     4561 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     4621 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     4681 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     4741 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     4801 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     4861 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     4921 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     4981 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     5041 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     5101 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     5161 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     5221 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     5281 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     5341 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     5401 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     5461 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     5521 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     5581 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     5641 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     5701 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     5761 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     5821 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     5881 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     5941 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     6001 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     6061 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     6121 tctatccggc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     6181 aagaagtacg gcggcttcct gtggcccacc gtggcctatt ctgtgctggt ggtggccaaa
     6241 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctactggg gatcaccatc
     6301 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     6361 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     6421 ggccggaaga gaatgctggc ctctgccaag cagctgcaga agggaaacga actggccctg
     6481 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     6541 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     6601 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     6661 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     6721 atcatccacc tgtttaccct gacccggctg ggagcccctc gggccttcaa gtactttgac
     6781 accaccatcg accccaagca gtaccggagc accaaagagg tgctggacgc caccctgatc
     6841 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     6901 aagcgacctg ccgccacaaa gaaggctgga caggctaaga agaagaaaga ttacaaagac
     6961 gatgacgata agggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc
     7021 gaagagaatc ctggaccgat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     7081 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     7141 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     7201 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     7261 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     7321 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     7381 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagaa
     7441 ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga aagattgact
     7501 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7561 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7621 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7681 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7741 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7801 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7861 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7921 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7981 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8041 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     8101 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8161 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     8221 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8281 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     8341 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8401 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8461 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8521 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8581 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8641 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8701 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     8761 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8821 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8881 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8941 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9001 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     9061 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     9121 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9181 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9241 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9301 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9361 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9421 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9481 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9661 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9721 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     9781 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     9841 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     9901 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     9961 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
    10021 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
    10081 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
    10141 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
    10201 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
    10261 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
    10321 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    10381 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    10441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    10561 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10741 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    11221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    11461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    12001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    12061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    12121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    12181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    12241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    12301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    12361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    12421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    12481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12721 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
    12781 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctc
//
