LOCUS       sequence-390307-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with
            an N-terminus His tag followed by a TEV cleavage site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Ghelichkhani F, Gonzalez FA, Kapitonova MA, Rozovsky S
  TITLE     Selenoprotein S Interacts with the Replication and Transcription 
            Complex of SARS-CoV-2 by Binding nsp7.
  JOURNAL   J Mol Biol. 2023 Apr 15;435(8):168008. doi: 
            10.1016/j.jmb.2023.168008. Epub 2023 Feb 10.
  PUBMED    36773692
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2023-04-15"; volume: "435(8):168008. doi"; pages: " 
            10.1016/j.jmb.2023.168008. Epub 2023 Feb 10"
COMMENT     Sequence Label: pET-28a(+)-6xHis-TEV-ORF9c
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..85
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     169..188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        169..187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    188..212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             227..249
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             268..285
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             295..315
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             587..604
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(657..675)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      671..718
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
       61 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      121 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
      181 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      241 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcgagaat
      301 ctttattttc agggccatat gctacaatca tgttataatt ttttaaaaga acaacactgc
      361 cagaaggcta gcacccagaa gggcgcagaa gcggctgtga aaccgctgtt ggttccgcat
      421 cacgtcgtgg ccaccgttca ggagatccaa ctgcaagcgg cggttggtga actgttactc
      481 ttggagtggc tggcaatggc ggtgatgctg ttgctgctgt gttgctgcct gacggactaa
      541 ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca
      601 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      661 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      721 gaaaggagga actatatccg gat
//
