LOCUS       sequence-390309-        2076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 5T empty.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-5T-Empty
FEATURES             Location/Qualifiers
     source          1..2076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..245
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1330..1918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1819..1838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2072..2076,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cctcggcgcg ccggagagat
       61 cgcgcacgcc cgctgagctc agtgttttgc aggtgtgatg aaaagcctta gggtcaggtg
      121 gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa
      181 atatgtatcc gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga
      241 agagtattga aaaaggaaga gtatgagtat ccaacatttc cgtgtcgccc ttattccctt
      301 ttttgcggcc ttctgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      361 tgctgaagat cagttgggtg cccgagtggg ttacattgaa ctggatctca acagcggcaa
      421 aatcttagag agttttcgcc ccgaagaacg cttcccaatg atgagcactt tcaaagttct
      481 gctatgtggc gcggtgttat cccgtattga tgctgggcaa gagcaactcg gtcgccgcat
      541 acactattct cagaatgact tggttgagta ttcaccagtc acagaaaagc atcttacgga
      601 tggcatgaca gtaagagaat tatgtagtgc tgccataacc atgagtgata acactgcggc
      661 caacttactt ctgacaacga ttggaggacc gaaggagcta accgcttttt tgcacaacat
      721 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      781 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca actctccgca aactattaac
      841 tggcgaacta cttactcttg cttcacgcca gcaactcatt gactggatgg aggcggacaa
      901 agttgcagga ccacttctgc gctcggcact tccggctggc tggtttattg ctgataaatc
      961 tggagccggt gagcgtggct ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1021 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1081 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag acgaagggaa
     1141 ataaatagta gcccgcctga tgtgcgggct tttttttggt ggtggttacc agtgtcgctt
     1201 agctaccgta ggtcctctta ggcggtccga ttagcgacat agtcatcaat gccttgacgg
     1261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1381 accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt ttccgaaggt
     1441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc ccagcttgga
     1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2041 taccgctcgc cgcagccgaa cgaccgagcg cagcga
//
