LOCUS       sequence-390311-        2059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 2 empty.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-2-Empty
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..245
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1313..1901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1802..1821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2055..2059,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt agccggcgcg ccggagagat
       61 cgcgcacgcc cgctgagctc caccttttgc aggtgtgatg aaaagcctta gggtcaggtg
      121 gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa
      181 atatgtatcc gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga
      241 agagtatgag tatccaacat ttccgtgtcg cccttattcc cttttttgcg gccttctgcc
      301 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      361 gtgcccgagt gggttacatt gaactggatc tcaacagcgg caaaatctta gagagttttc
      421 gccccgaaga acgcttccca atgatgagca ctttcaaagt tctgctatgt ggcgcggtgt
      481 tatcccgtat tgatgctggg caagagcaac tcggtcgccg catacactat tctcagaatg
      541 acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      601 aattatgtag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      661 cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      721 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      781 cgatgcctgt agcaatggca acaactctcc gcaaactatt aactggcgaa ctacttactc
      841 ttgcttcacg ccagcaactc attgactgga tggaggcgga caaagttgca ggaccacttc
      901 tgcgctcggc acttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      961 gctctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1021 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1081 gtgcctcact gattaagcat tggtaactgt cagacgaagg gaaataaata gtagcccgcc
     1141 tgatgtgcgg gctttttttt ggtggtggtt accagtgtcg cttagctacc gtaggtcctc
     1201 ttaggcggtc cgattagcga catagtcatc aatgccttga cggcaaaatc ccttaacgtg
     1261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1381 tttgtttgcc ggatcaagag ctactaactc tttttccgaa ggtaactggc ttcagcagag
     1441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1621 ggtcgggctg aacggggggt tcgtgctcac agcccagctt ggagcgaacg acatacaccg
     1681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2041 gaacgaccga gcgcagcga
//
