LOCUS       sequence-390320-       16431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GCN1 R2312A expresson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 16431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP
FEATURES             Location/Qualifiers
     source          1..16431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1715..2018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2019..2222
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2173..2193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2219..2243
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2339..2363
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10378..10443
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    10448..11020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10615..10632)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10842..10861
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11038..11973
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    12034..12622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12087..12107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12505..12516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12723..12956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13028..13149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13065..13084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13119..13138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13189..13324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13344..13363)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13345..13363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13373..13390)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13373..13389)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13382..13404)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13531..13986
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13618..13637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13828..13849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14012..14116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14117..14185,14186..14977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14335..14354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15148..15736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15637..15656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15890..15907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16024..16045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16060..16065,16066..16083,16084..16090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16098..16114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16103..16125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16122..16138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16122..16138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16157..16177
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16159..16177
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        16205..16431
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc
     1741 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1801 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1861 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1921 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1981 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2041 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2101 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2161 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2221 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2281 gaagacaccg actctagagg atccactagt ccagtgtggt ggaattctgc agatatcaac
     2341 aagtttgtac aaaaaagcag gcttatggcg gcggacacgc aggtttccga gacactaaag
     2401 cgttttgcag ggaaggtgac aacagccagt gtaaaggaac ggagagaaat cctcagtgaa
     2461 cttgggaagt gtgttgctgg aaaagatctt ccagagggag cagtgaaggg gctctgcaaa
     2521 ttgttctgct tgactctgca tcgatataga gatgcagcct cccgcagggc cttgcaggca
     2581 gccatccagc agttggctga ggcccagcca gaagccactg ctaagaacct tctacactct
     2641 ctgcagtctt ctggtatagg ctccaaagca ggtgttccca gtaagagcag tggctctgcc
     2701 gccttgctgg ccttgacctg gacctgcctc ctggtgcgca ttgtctttcc atcgagagcc
     2761 aagcgacaag gagacatctg gaacaaactg gtggaagtgc agtgcctgct cttgctggag
     2821 gtgctgggtg gctcccacaa gcacgccgtg gatggtgctg tgaagaaact cacgaagctg
     2881 tggaaagaga accccgggct ggtggaacag tacttgtcag ccattctcag cctagagccc
     2941 aaccagaact atgctggcat gctggggctg ctggtgcagt tctgcacgag tcacaaggag
     3001 atggacgtgg tcagtcagca caagagcgcc ctactggact tttacatgaa gaacatcctg
     3061 atgagcaaag tcaagcctcc gaagtacctg ttggatagct gtgcccctct gctccgatac
     3121 ctgtcccact cagaatttaa ggatctgata ctgcccacca tacagaagtc cttactgagg
     3181 agtccagaga atgttattga aactatttct agtctgctgg catcagtgac gcttgacctc
     3241 agccagtatg ccatggacat cgtgaaagga ctggctggtc acctgaaatc caacagtccc
     3301 cgcctgatgg atgaagctgt gctggcactg cggaacctgg cacgccagtg cagtgactct
     3361 tcggccatgg aatccctgac caagcaccta tttgctatcc tcggaggctc ggaaggaaaa
     3421 ctaactgttg tagcccagaa gatgagcgtc ctctcaggga ttgggagcgt cagtcatcac
     3481 gtggtgtctg gaccttccag tcaggtcctg aatgggatcg tggctgagct gttcatcccg
     3541 ttccttcagc aggaagttca tgaagggacc ttggtacacg ctgtctcagt cctggctctc
     3601 tggtgtaacc gattcactat ggaagtgccc aagaagctca ctgaatggtt caaaaaagct
     3661 ttcagcctta aaacctccac atctgcggtg aggcatgcct acctgcagtg catgttggcc
     3721 tcttaccggg gtgacacgct gttgcaggcc ctggacttac tgcccttgct catccagaca
     3781 gtggagaagg cagcctccca aagcactcag gttcccacca tcactgaagg ggttgccgca
     3841 gccttgttgc tcttaaagtt gtcagtggct gactcacagg ctgaggccaa actgagcagt
     3901 ttctggcagt tgattgtgga tgagaaaaag caggttttca cttctgagaa attcctggtc
     3961 atggcttcag aggatgccct gtgtactgtg ttgcatctga cagagagact tttccttgac
     4021 cacccgcata gactcactgg caacaaagtt cagcagtacc accgggctct ggtggcggtg
     4081 ctcctgagcc gcacctggca cgtccgcagg caggctcagc agacagttcg gaagctgctg
     4141 tcctctcttg ggggctttaa gctggcgcac ggactcttgg aggagctgaa gactgtcctc
     4201 agttctcaca aggtgctgcc cttagaggct ttggtgactg atgctggaga ggtgactgag
     4261 gcaggcaagg cctacgtgcc tccacgggtc ctgcaggagg ctctgtgtgt catctccggt
     4321 gtgccagggc tcaagggtga tgtcaccgac actgaacaac tggcccagga aatgctgatc
     4381 atctcccacc acccatcctt agttgccgtg cagtctggac tttggccagc acttcttgcc
     4441 aggatgaaga tcgatcctga agcctttatc accaggcacc tggatcagat cattcccagg
     4501 atgaccacac agagtcccct aaaccagtcc tccatgaatg ccatgggctc cctttccgtc
     4561 ctgtcgccgg accgggtcct cccacagctc atcagcacca tcactgcctc cgtgcagaac
     4621 cctgcactgc gcctggtgac gcgggaggag tttgccatta tgcagacccc tgctggggag
     4681 ctgtatgaca aatccatcat tcagagtgcc cagcaggaca gcataaaaaa ggccaacatg
     4741 aagcgagaga acaaagctta ttccttcaaa gagcagatca tcgagctgga gctgaaggag
     4801 gagataaaga agaagaaagg catcaaagag gaggtgcagc tgaccagcaa gcagaaggag
     4861 atgctgcagg cccagctaga cagggaggcg caggtccgga ggcggctgca ggagctggat
     4921 ggggagctgg aggcggcgct tggactgctg gacatcatcc tggccaagaa cccgtccggc
     4981 ctgacccagt acatccctgt tttggtcgac tcttttctgc ccttgctgaa gtctcccctg
     5041 gctgctccca ggatcaagaa ccccttcttg tccttggctg cctgtgtcat gccctctagg
     5101 ctcaaggctt tgggcacttt ggtgagccac gtgaccctgc gcctgctgaa gccagagtgt
     5161 gtcctggata agtcctggtg ccaggaagag ctgtcggtgg ctgtgaagag ggcggtgatg
     5221 ctgctgcaca cccacaccat caccagcagg gtgggcaagg gggagccagg tgctgcgccc
     5281 ttgtccgcgc cagccttctc cttagtcttc ccgtttctga agatggtgct gacggagatg
     5341 ccccaccaca gtgaggagga ggaggagtgg atggcccaga ttcttcagat cctcactgtc
     5401 caagcccagc tgagggcctc ccccaacacc ccacccgggc gggtggacga gaatggcccg
     5461 gagttgctgc ctcgcgtggc catgctgcgt cttctgactt gggtgatcgg gacgggctcg
     5521 cctcgcttac aggttctggc ttcagacacc ctgaccaccc tgtgtgccag cagcagtggt
     5581 gatgatggct gtgcctttgc agagcaggag gaggtggacg tgctgctctg tgccttgcag
     5641 tccccgtgtg ccagcgtgcg ggaaaccgtg ctccgggggc tgatggaact ccacatggta
     5701 ttgccagcac ctgatactga tgagaagaat ggcctgaacc ttctgcggag actctgggtg
     5761 gtcaagtttg acaaggagga ggagatccgg aagctggctg agaggctctg gtcaatgatg
     5821 ggcctagacc tgcagccaga cctctgctcc ttgctgattg acgacgtgat ctatcatgag
     5881 gcggctgtaa ggcaggcagg ggccgaagcc ctctcccaag cagtggcacg ttaccagcgg
     5941 caggcggcgg aggttatggg caggctcatg gagatttacc aggaaaagct ctaccggccg
     6001 cccccagtgc tggatgcttt gggacgagtt atttcagaat ctcctccaga tcagtgggaa
     6061 gccaggtgtg gcttggcgtt ggccctcaac aagctctccc agtatttgga cagctctcag
     6121 gtgaagccac tctttcagtt ttttgtccct gatgccctca atgaccgaca cccagatgtc
     6181 cggaagtgca tgttggatgc agccctcgca acgctcaaca ctcatgggaa ggagaacgtc
     6241 aactcgctgt tgccagtatt cgaggagttc ctgaagaacg cgcccaatga tgccagctat
     6301 gatgctgtgc gacagagtgt ggtggtcctg atgggctctc tggccaagca cctggacaag
     6361 agtgacccca aagtgaagcc cattgttgcc aagctcatcg ctgccctctc caccccctcc
     6421 cagcaggtcc aggagtccgt agccagctgc ttgccacccc ttgtgccagc catcaaggag
     6481 gatgctggag ggatgatcca gaggcttatg cagcagctgc tggagtcaga caagtacgca
     6541 gagcgcaaag gggccgccta tggcctggcg ggcctggtga agggcctggg catcctctcg
     6601 ctgaagcaac aggagatgat ggcggcactg actgatgcca tccaagataa gaagaacttc
     6661 cgccggcgag agggagccct ctttgccttc gagatgctct gcaccatgct ggggaaactt
     6721 tttgagccgt atgtggttca cgtgctgccc catctgctcc tgtgctttgg ggatggaaac
     6781 cagtatgtgc gtgaggctgc agatgactgt gccaaggctg tgatgagcaa cttgagtgct
     6841 cacggggtga agctggtgct cccctcctta ctggctgccc tggaggagga atcgtggcgg
     6901 accaaagctg ggtcagtgga gcttcttggg gcaatggcgt actgtgctcc taagcagctg
     6961 tcatcctgtc tacccaacat tgtgcccaag cttacggagg tgctgaccga ctcccatgtc
     7021 aaagtccaga aggctggaca gcaggcgctc aggcagatcg gctccgttat caggaacccg
     7081 gagatcctgg ccattgctcc agtcctcctg gatgccctga cggatccctc caggaagacc
     7141 cagaagtgct tgcagaccct gctggacacc aagtttgtcc acttcattga tgccccatcc
     7201 ctggccctca tcatgcccat tgtccagaga gccttccagg accgttccac ggacacgcgg
     7261 aagatggcag cccagattat tggcaacatg tactccctga cagaccagaa ggacttggct
     7321 ccgtacctgc ccagcgtgac gcctggcctg aaagcatcgc ttttggaccc tgtgcctgag
     7381 gtgcggaccg tatctgcaaa ggcccttggg gccatggtga agggcatggg ggagtcgtgc
     7441 tttgaggact tgctgccgtg gctgatggag acactgacct atgagcagag ctctgtggat
     7501 cgctcaggcg ctgcacaggg gttggctgag gtcatggccg gtttgggggt ggagaagttg
     7561 gagaagttga tgccagaaat cgtggctaca gccagcaaag tggacattgc accccatgtc
     7621 cgagatggct acattatgat gtttaactac ctgcccatca cctttggaga caagtttact
     7681 ccttatgtgg ggcccatcat cccctgtatc ctcaaagctc ttgctgatga gaatgagttt
     7741 gtgcgtgaca ccgccctgcg cgcgggccag cgggttatct ccatgtacgc tgagacagcc
     7801 atcgccctgc tgctgcccca gctagagcaa ggcctctttg atgacctttg gagaatcagg
     7861 ttcagctctg ttcagctcct tggggatctc ctgtttcaca tctcaggagt cactgggaag
     7921 atgaccacag aaactgcctc tgaggatgat aactttggaa ctgcccagtc caacaaggcg
     7981 atcatcactg ccctgggggt agagcggcgg aaccgggtgt tggcagggct gtacatgggc
     8041 cgctcagaca cccagctggt ggtgcggcag gcgtccctgc atgtctggaa gattgttgtc
     8101 tccaataccc cccgcacctt gcgtgagatc ctacccactc tctttgggct cctgctgggt
     8161 ttcctggcca gcacgtgtgc agataagaga acgattgcag cgagaacatt gggagatctt
     8221 gtgcggaagt taggggagaa aatcctcccc gagatcatcc ccatccttga ggaaggcctg
     8281 aggtctcaga agagcgatga gaggcagggt gtgtgcattg gcctaagtga gatcatgaag
     8341 tccaccagcc gggatgccgt gctgtatttc tctgaatccc tcgtgcccac ggcaaggaag
     8401 gctttgtgtg acccactgga ggaggtcaga gaggcggcag ccaagacttt cgagcagctg
     8461 cattccacca tcggccacca ggctctggag gacattctcc catttttact aaagcagctg
     8521 gatgacgagg aggtgtcaga gtttgccttg gatggtctga agcaagtcat ggctattaag
     8581 agtcgtgtgg tgctgcccta ccttgtgccc aagctgacaa cgccacctgt caacacccgg
     8641 gtgctggctt tcctttcgtc agtggctggt gatgccctca cccgtcatct tggcgtgatc
     8701 ctcccagcgg tcatgctggc cctgaaggaa aagcttggga ccccagatga gcagctggag
     8761 atggccaatt gtcaggctgt gatcctctcc gtagaggatg acacagggca ccggatcatc
     8821 atcgaggatc tgctggaggc cacccgcagc cctgaggtgg gcatgaggca agctgctgcc
     8881 atcatcctca acatctactg ttcccgctca aaggctgact acaccagcca cctgcggagc
     8941 ctggtctcgg gcctgatccg cctcttcaat gactccagcc ctgtggttct ggaggagagc
     9001 tgggatgccc taaatgccat cactaagaag ctggatgctg gcaaccagtt ggcactcatt
     9061 gaagagctgc acaaggaaat ccggctcata gggaacgaga gcaaaggcga gcatgtgcca
     9121 ggattctgcc tcccgaagaa gggagtgacc tccatccttc cagtgttgcg ggaaggagtc
     9181 ctgactggca gccctgagca gaaggaggag gcagccaaag ccttaggctt ggtaatccgc
     9241 ctgacctcgg ctgacgccct gaggccctcc gtggtcagca tcactggccc tctgatcgcg
     9301 atcctggggg acaggttcag ctggaatgtg aaggcggctc tgctcgagac actcagcctc
     9361 ttgttggcta aggttgggat tgccctgaag cccttcctgc cccagctgca gaccactttc
     9421 accaaagccc tgcaggactc caaccggggg gtgcgcctga aggccgcaga tgctctgggg
     9481 aagctcattt ccatccacat taaggtggac cccctcttca cagagctgct caatggcatc
     9541 cgcgccatgg aggacccagg tgtcagggac accatgctgc aggccctgag gtttgtgatt
     9601 cagggagcag gggccaaagt ggatgccgtc atccggaaaa acatcgtctc actcctgctg
     9661 agcatgctgg gacacgatga ggacaacact cgcatctcct cagccgggtg cctaggggaa
     9721 ctgtgtgcct ttttgactga agaggagctt agtgccgttc tacagcagtg cttgctggcg
     9781 gacgtgtccg gcattgactg gatggttcgg cacgggcgga gcctggcact ttccgtggct
     9841 gtgaatgtgg ctcctggcag actttgtgcc ggcagatata gcagtgatgt tcaggaaatg
     9901 atcctgagca gtgccacggc ggacaggatc cccattgcgg tgagcggggt ccggggcatg
     9961 ggctttctca tgagacacca catcgagaca ggcggagggc agttgccggc caaactttcc
    10021 agcctgttcg ttaagtgtct gcagaaccca tccagcgaca tcaggctggt ggctgagaag
    10081 atgatctggt gggcaaataa ggacccactg cctcccctgg acccccaggc catcaagccc
    10141 atcctgaagg ctcttcttga caacaccaag gataagaaca ccgtggtcag ggcctacagc
    10201 gaccaggcaa ttgtcaacct cctcaagatg cggcagggtg aagaggtgtt tcagtccctc
    10261 tccaagatcc tggatgtggc cagtttggag gtgctgaacg aggttaaccg aaggtccctg
    10321 aagaagctgg ccagccaggc cgactccacg gagcaggtgg acgacaccat cctgacagac
    10381 tacaaagacc acgacggtga ctataaggac cacgacatcg actacaaaga cgatgacgac
    10441 aagtaggccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
    10501 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
    10561 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
    10621 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
    10681 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
    10741 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
    10801 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
    10861 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
    10921 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
    10981 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgtatcg atccaccatg
    11041 gcgcgtaagg tcgatctcac ctcctgcgat cgcgagccga tccacatccc cggcagcatt
    11101 cagccgtgcg gctgcctgct agcctgcgac gcgcaggcgg tgcggatcac gcgcattacg
    11161 gaaaatgccg gcgcgttctt tggacgcgaa actccgcggg tcggtgagct actcgccgat
    11221 tacttcggcg agaccgaagc ccatgcgctg cgcaacgcac tggcgcagtc ctccgatcca
    11281 aagcgaccgg cgctgatctt cggttggcgc gacggcctga ccggccgcac cttcgacatc
    11341 tcactgcatc gccatgacgg tacatcgatc atcgagttcg agcctgcggc ggccgaacag
    11401 gccgacaatc cgctgcggct gacgcggcag atcatcgcgc gcaccaaaga actgaagtcg
    11461 ctcgaagaga tggccgcacg ggtgccgcgc tatctgcagg cgatgctcgg ctatcaccgc
    11521 gtgatgttgt accgcttcgc ggacgacggc tccgggatgg tgatcggcga ggcgaagcgc
    11581 agcgacctcg agagctttct cggtcagcac tttccggcgt cgctggtccc gcagcaggcg
    11641 cggctactgt acttgaagaa cgcgatccgc gtggtctcgg attcgcgcgg catcagcagc
    11701 cggatcgtgc ccgagcacga cgcctccggc gccgcgctcg atctgtcgtt cgcgcacctg
    11761 cgcagcatct cgccctgcca tctcgaattt ctgcggaaca tgggcgtcag cgcctcgatg
    11821 tcgctgtcga tcatcattga cggcacgcta tggggattga tcatctgtca tcattacgag
    11881 ccgcgtgccg tgccgatggc gcagcgcgtc gcggccgaaa tgttcgccga cttcttatcg
    11941 ctgcacttca ccgccgccca ccaccaacgc taaagcggcc gcgactctag agtcgacctg
    12001 caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt
    12061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    12121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    12181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    12241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    12301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    12361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    12421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    12481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    12541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    12601 tccctttggg ccgcctcccc gcctggcgat ggtaccgcac aattcgagct cggtaccttt
    12661 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
    12721 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
    12781 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    12841 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    12901 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
    12961 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
    13021 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13141 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
    13201 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    13261 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    13321 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
    13381 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    13441 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    13501 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
    13561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    13621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    13681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    13741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    13801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    13861 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    13921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    13981 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    14041 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    14101 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    14161 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    14221 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    14281 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    14341 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    14401 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    14461 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    14521 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    14581 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    14641 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    14701 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    14761 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    14821 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    14881 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    14941 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    15001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    15061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    15121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    15181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    15241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    15301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    15361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    15421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    15481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    15541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    15601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    15661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    15721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    15781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    15841 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    15901 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    15961 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    16021 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    16081 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    16141 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
    16201 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
    16261 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
    16321 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
    16381 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa c
//
