LOCUS       sequence-39034-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pcDNA4-Beclin1-(151-241)
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    23..144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(34..53)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     88..107
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(274..648)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(667..714)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac cacaactaga
       61 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      121 attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg
      181 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
      241 ggcggcggtg gaatcgaaat ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg
      301 cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat
      361 ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc
      421 ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac
      481 ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc
      541 gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc
      601 gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg tttagttcct
      661 caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt caacacgtgc
      721 tgatcagatc cgaaaatgga tatacaagct cccgggagct ttttg
//
