LOCUS       sequence-390363-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible expression of NINJ1 gene  in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Borges JP, Saetra RSR, Volchuk A, Bugge M, Devant P, Sporsheim B, 
            Kilburn BR, Evavold CL, Kagan JC, Goldenberg NM, Flo TH, Steinberg 
            BE
  TITLE     Glycine inhibits NINJ1 membrane clustering to suppress plasma 
            membrane rupture in cell death.
  JOURNAL   Elife. 2022 Dec 5;11:e78609. doi: 10.7554/eLife.78609.
  PUBMED    36468682
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-5"; volume: "11:e78609. doi"; pages: " 
            10.7554/eLife.78609"
COMMENT     Sequence Label: pRetroX-TRE3G_NINJ1
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..36
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccgccacca tggactacaa agacgatgac gacaaggagt ctggcaccga ggaatatgag
       61 ctgaacggtg atttgcgccc tggctctcct ggttctcctg atgccctccc accaaggtgg
      121 gggcttcgga atcgccctat caatgtaaat cactacgcaa ataagaaatc agcagcagag
      181 tctatgctgg acattgcact cttgatggct aacgcttctc agttgaaggc tgtggtagag
      241 caaggaaacg attttgcatt ttttgtcccc ttggtggtat tgatttcaat aagcctggta
      301 cttcagattg gagtcggggt actcctgatc ttcttggtga aatatgacct caacaatcca
      361 gccaaacacg caaagctgga ttttcttaat aatctcgcaa ccggactggt atttattatc
      421 gtagtcgtta acatttttat caccgcattt ggggtccaga agccagtgat ggatgtcgcc
      481 ccaagacaat ag
//
