LOCUS       sequence-390366-        5589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 SARS-CoV-1 NSP12_nostop
FEATURES             Location/Qualifiers
     source          1..5589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2900..2999)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3016..3035)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3017..3035)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3040..3056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3040..3056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3487..4278
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4371..4959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4860..4879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5113..5130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5289..5316)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5408..5494)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5543..5565
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5557..5574
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5558..5574
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtctgcgga tgcatcaacg
      121 tttttaaacc gggtttgcgg tgtaagtgca gcccgtctta caccgtgcgg cacaggcact
      181 agtactgatg tcgtctacag ggcttttgat atttacaacg aaaaagttgc tggttttgca
      241 aagttcctaa aaactaattg ctgtcgcttc caggagaagg atgaggaagg caatttatta
      301 gactcttact ttgtagttaa gaggcatact atgtctaact accaacatga agagactatt
      361 tataacttgg ttaaagattg tccagcggtt gctgtccatg actttttcaa gtttagagta
      421 gatggtgaca tggtaccaca tatatcacgt cagcgtctaa ctaaatacac aatggctgat
      481 ttagtctatg ctctacgtca ttttgatgag ggtaattgtg atacattaaa agaaatactc
      541 gtcacataca attgctgtga tgatgattat ttcaataaga aggattggta tgacttcgta
      601 gagaatcctg acatcttacg cgtatatgct aacttaggtg agcgtgtacg ccaatcatta
      661 ttaaagactg tacaattctg cgatgctatg cgtgatgcag gcattgtagg cgtactgaca
      721 ttagataatc aggatcttaa tgggaactgg tacgatttcg gtgatttcgt acaagtagca
      781 ccaggctgcg gagttcctat tgtggattca tattactcat tgctgatgcc catcctcact
      841 ttgactaggg cattggctgc tgagtcccat atggatgctg atctcgcaaa accacttatt
      901 aagtgggatt tgctgaaata tgattttacg gaagagagac tttgtctctt cgaccgttat
      961 tttaaatatt gggaccagac ataccatccc aattgtatta actgtttgga tgataggtgt
     1021 atccttcatt gtgcaaactt taatgtgtta ttttctactg tgtttccacc tacaagtttt
     1081 ggaccactag taagaaaaat atttgtagat ggtgttcctt ttgttgtttc aactggatac
     1141 cattttcgtg agttaggagt cgtacataat caggatgtaa acttacatag ctcgcgtctc
     1201 agtttcaagg aacttttagt gtatgctgct gatccagcta tgcatgcagc ttctggcaat
     1261 ttattgctag ataaacgcac tacatgcttt tcagtagctg cactaacaaa caatgttgct
     1321 tttcaaactg tcaaacccgg taattttaat aaagactttt atgactttgc tgtgtctaaa
     1381 ggtttcttta aggaaggaag ttctgttgaa ctaaaacact tcttctttgc tcaggatggc
     1441 aacgctgcta tcagtgatta tgactattat cgttataatc tgccaacaat gtgtgatatc
     1501 agacaactcc tattcgtagt tgaagttgtt gataaatact ttgattgtta cgatggtggc
     1561 tgtattaatg ccaaccaagt aatcgttaac aatctggata aatcagctgg tttcccattt
     1621 aataaatggg gtaaggctag actttattat gactcaatga gttatgagga tcaagatgca
     1681 cttttcgcgt atactaagcg taatgtcatc cctactataa ctcaaatgaa tcttaagtat
     1741 gccattagtg caaagaatag agctcgcacc gtagctggtg tctctatctg tagtactatg
     1801 acaaatagac agtttcatca gaaattattg aagtcaatag ccgccactag aggagctact
     1861 gtggtaattg gaacaagcaa gttttacggt ggctggcata atatgttaaa aactgtttac
     1921 agtgatgtag aaactccaca ccttatgggt tgggattatc caaaatgtga cagagccatg
     1981 cctaacatgc ttaggataat ggcctctctt gttcttgctc gcaaacataa cacttgctgt
     2041 aacttatcac accgtttcta caggttagct aacgagtgtg cgcaagtatt aagtgagatg
     2101 gtcatgtgtg gcggctcact atatgttaaa ccaggtggaa catcatccgg tgatgctaca
     2161 actgcttatg ctaatagtgt ctttaacatt tgtcaagctg ttacagccaa tgtaaatgca
     2221 cttctttcaa ctgatggtaa taagatagct gacaagtatg tccgcaatct acaacacagg
     2281 ctctatgagt gtctctatag aaatagggat gttgatcatg aattcgtgga tgagttttac
     2341 gcttacctgc gtaaacattt ctccatgatg attctttctg atgatgccgt tgtgtgctat
     2401 aacagtaact atgcggctca aggtttagta gctagcatta agaactttaa ggcagttctt
     2461 tattatcaaa ataatgtgtt catgtctgag gcaaaatgtt ggactgagac tgaccttact
     2521 aaaggacctc acgaattttg ctcacagcat acaatgctag ttaaacaagg agatgattac
     2581 gtgtacctgc cttacccaga tccatcaaga atattaggcg caggctgttt tgtcgatgat
     2641 attgtcaaaa cagatggtac acttatgatt gaaaggttcg tgtcactggc tattgatgct
     2701 tacccactta caaaacatcc taatcaggag tatgctgatg tctttcactt gtatttacaa
     2761 tacattagaa agttacatga tgagcttact ggccacatgt tggacatgta ttccgtaatg
     2821 ctaactaatg ataacacctc acggtactgg gaacctgagt tttatgaggc tatgtacaca
     2881 ccacatacag tcttgcagta cccaactttc ttgtacaaag ttggcattat aagaaagcat
     2941 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     3001 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     3061 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     3121 tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc
     3181 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg
     3241 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     3301 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     3361 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     3421 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     3481 aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     3541 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     3601 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     3661 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     3721 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     3781 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     3841 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     3901 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     3961 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     4021 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     4081 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     4141 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     4201 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     4261 aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac
     4321 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4381 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4441 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4501 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4561 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4621 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4681 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4741 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4801 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4861 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4921 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4981 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5041 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5101 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5161 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5221 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     5281 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     5341 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     5401 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     5461 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     5521 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     5581 ctcgggccc
//
