LOCUS       sequence-390372-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-HKU1 NSP12_stop
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2891..2990)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3007..3026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3008..3026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3031..3047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3031..3047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3478..4269
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4362..4950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4851..4870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5104..5121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5280..5307)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5399..5485)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5534..5556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5548..5565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5549..5565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtctaaaga tttaaatttt
      121 ttaaaccggg ttcggggtac tagtgtgaat gcccggctag taccctgtgc tagtggttta
      181 tctactgatg ttcaattaag ggcatttgac atttgtaata ccaatagagc tggtataggt
      241 ttatattata aagtgaattg ttgccgtttt cagcgtatag atgacgacgg taataaattg
      301 gataagttct ttgttgtcaa aagaactaat ttagaagttt ataataaaga gaaaacttat
      361 tatgagttga ctaaaagttg tggtgttgtg gctgaacatg atttctttac atttgatatt
      421 gatggtagtc gcgtgccaca tatagttcgt aggaatcttt caaagtatac tatgttagat
      481 ctttgctatg cattgcgtca ttttgatcgt aatgattgtt caatattgtg tgaaattctt
      541 tgtgagtatg ctgattgtaa agaatcctac ttttctaaga aagattggta tgattttgtt
      601 gaaaatcctg atattattaa tatatataaa aaattaggcc ctatttttaa tagagcttta
      661 cttaatactg tcatttttgc agacacctta gttgaagtag gtttagttgg tgttttaact
      721 ttagataacc aagatttgta tggtcaatgg tatgattttg gtgattttat acaaacagcc
      781 ccagggtttg gtgtggcagt tgcagattct tactattctt atatgatgcc tatgttgact
      841 atgtgtcatg tattagattg tgaattattt gttaatgata gttatagaca attcgatctt
      901 gtacagtatg attttactga ttacaagtta gagttgttta ataagtattt taagtattgg
      961 ggtatgaagt atcatcctaa tactgtggat tgtgataatg ataggtgtat tattcattgt
     1021 gctaatttta atatactatt tagtatggtt ttacctaata cttgttttgg tccccttgtt
     1081 agacaaattt ttgtagatgg tgtaccgttt gttgtttcta ttggttacca ttacaaagag
     1141 ttaggtgtag ttatgaactt agatgttgac acacaccgtt atcgtttgtc tcttaaagat
     1201 ttacttcttt atgcagcaga tcctgctatg cacgttgcat ctgctagtgc tctgcttgat
     1261 ttacgaactt gttgttttag tgtagctgcc attacaagtg gtataaaatt tcaaactgta
     1321 aaaccaggta actttaacca agacttttac gagtttgtta aaagtaaagg cttgtttaaa
     1381 gagggtagta cagttgattt gaaacatttt ttctttactc aagatggtaa tgctgcaatt
     1441 actgattata attattataa gtataattta cctactatgg ttgatattaa gcagttattg
     1501 tttgtattag aagttgttta taaatatttt gaaatttatg atggtggttg tataccagca
     1561 tcacaagtta ttgttaataa ttatgataaa agtgctggtt atccatttaa taaatttggt
     1621 aaagccagac tttattatga ggcattatca tttgaggaac agaatgaaat ttatgcatat
     1681 actaaacgta atgttctgcc caccttaact caaatgaatt taaaatatgc tatcagtgct
     1741 aagaatagag ctcgcactgt agcaggtgtt tctattctta gtactatgac aggccgaatg
     1801 ttccatcaaa aatgtttgaa gagtatagca gctacccgag gtgttcctgt tgttatagga
     1861 accactaaat tttatggtgg ttgggacgat atgttacgtc atcttataaa ggatgttgac
     1921 aaccctgttc ttatgggttg ggattatcct aaatgtgatc gtgctatgcc aaatattttg
     1981 cgtattgtta gtagtttagt tttggcccgc aaacatgaat tttgttgttc acatggtgat
     2041 agattttatc gccttgcgaa tgaatgtgct caagttttga gtgaaatagt tatgtgtggc
     2101 ggttgctatt atgttaagcc tggtggtact agcagtggtg atgcaactac tgcttttgct
     2161 aattctgttt ttaatatatg tcaggctgtt actgctaatg tttgttctct tatggcctgt
     2221 aatggccata agattgaaga tttaagtata cgcaatttac aaaaacgctt atactctaat
     2281 gtttatcgta cagattatgt tgattataca tttgttaatg agtattatga atttttatgt
     2341 aagcatttta gtatgatgat tttgagtgat gatggtgttg tctgttataa ctctgattat
     2401 gctagtaagg gttatatagc taatataagt gtttttcaac aagttttgta ctatcagaat
     2461 aatgtcttta tgtctgaatc taaatgttgg gttgaaaatg atattactaa tggtcctcat
     2521 gaattttgtt cccaacatac tatgttagtt aagatagatg gtgattatgt ttatttacca
     2581 tatccagatc cttctagaat tttaggagct ggttgttttg ttgatgattt attgaagact
     2641 gacagtgttc ttttgataga gcgctttgta agtctagcta tagatgctta ccctttagta
     2701 catcatgaaa atgaagaata ccaaaaagtc tttcgtgtat atttagaata tataaaaaaa
     2761 ctgtataatg atcttggtac tcagatctta gatagttata gtgttatttt aagtacttgt
     2821 gatggtttaa agtttactga agaatcattt tacaagaata tgtatttaaa aagtgccgtg
     2881 atgcagtaat acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2941 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     3001 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     3061 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     3121 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     3181 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     3241 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     3301 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     3361 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     3421 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     3481 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     3541 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     3601 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     3661 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     3721 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     3781 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     3841 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     3901 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     3961 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     4021 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     4081 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     4141 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     4201 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     4261 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     4321 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4501 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4981 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5041 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5101 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5161 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5221 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     5281 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     5341 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     5401 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     5461 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     5521 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
