LOCUS       sequence-390376-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 nsp7 in mammalian..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ghelichkhani F, Gonzalez FA, Kapitonova MA, Rozovsky S
  TITLE     Selenoprotein S Interacts with the Replication and Transcription 
            Complex of SARS-CoV-2 by Binding nsp7.
  JOURNAL   J Mol Biol. 2023 Apr 15;435(8):168008. doi: 
            10.1016/j.jmb.2023.168008. Epub 2023 Feb 10.
  PUBMED    36773692
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2023-04-15"; volume: "435(8):168008. doi"; pages: " 
            10.1016/j.jmb.2023.168008. Epub 2023 Feb 10"
COMMENT     Sequence Label: pcDNA 3.1-nsp7
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(421..438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    427..651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(784..803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccgggat gtctaagatg agtgatgtga aatgtacgtc cgtggtgttg ctttctgtcc
      181 tgcaacagct tagagttgaa tctagcagta agctctgggc acaatgcgtg caactccaca
      241 atgatattct gttggcgaaa gatactactg aagccttcga gaagatggta tccttgttgt
      301 ctgtcctttt gagcatgcaa ggcgcggttg acatcaacaa gttgtgtgag gagatgctgg
      361 ataaccgagc tacactgcaa tagctcgagt ctagagggcc cgtttaaacc cgctgatcag
      421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      541 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgang
      661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
      721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctancgc
      781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      841 ctctaaatcg ggggctccct ttagggttcc natttagtgc tttacggcac ctcgacccca
      901 aaaactnatt nnntgatggn tcacgtantg gncatcgccc tgatagangt tttcgccctt
      961 tgacntnnnt ccacgttctt tnntantgga ntcntntncn aactggnaac nnnncntcan
     1021 nnnntnnt
//
