LOCUS       sequence-390411-        2154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express deltaLight biosensor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Dong C, Gowrishankar R, Jin Y, He XJ, Gupta A, Wang H, Sayar-Atasoy 
            N, Flores RJ, Mahe K, Tjahjono N, Liang R, Marley A, Or Mizuno G, Lo
            DK, Sun Q, Whistler JL, Li B, Gomes I, Von Zastrow M, Tejeda HA, 
            Atasoy D, Devi LA, Bruchas MR, Banghart MR, Tian L
  TITLE     Unlocking opioid neuropeptide dynamics with genetically encoded 
            biosensors.
  JOURNAL   Nat Neurosci. 2024 Jul 15. doi: 10.1038/s41593-024-01697-1.
  PUBMED    39009835
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jul 15. doi: 10.1038/s41593-024-01697-1."
COMMENT     Sequence Label: pCMV- delta-Light
FEATURES             Location/Qualifiers
     source          1..2154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1035..1056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1162..1183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1423..1442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgaagacga tcatcgccct gagctacatc ttctgcctgg tgttcgccga ctacaaggac
       61 gatgatgacg ccatggaacc agcccctagt gcaggcgcag aattgcagcc ccctctgttt
      121 gccaacgcaa gtgacgccta cccttccgcg tgccccagcg caggggctaa cgccagcggc
      181 cctccggggg caagaagcgc tagcagtctg gccctggcta ttgccattac cgccctgtat
      241 tccgccgtgt gtgcagtggg acttctgggt aacgtgctgg tgatgttcgg gattgtgcgc
      301 tacaccaaga tgaaaaccgc aaccaacatt tacattttta acctggcttt ggccgacgca
      361 ctggccacca gtaccctgcc attccaaagc gccaaatacc tgatggaaac gtggccgttt
      421 ggggagttgc tgtgtaaggc cgtgctgagc attgattact acaacatgtt tacctccatc
      481 tttactctga caatgatgag tgtggatcgg tatattgcag tctgccaccc aaagaaggcc
      541 ctggatttcc ggactcccgc aaaggccaaa ctgataaata tatgtatctg ggtcctggcc
      601 tcaggcgtgg gagtacccat tatggtgatg gctgtgacac ggccccggga tggggccgtt
      661 gtctgcatgc ttcaatttcc cagcccctcc tggtactggg ataccgtcac taagatctgt
      721 gtcttcctgt ttgcgtttgt ggtaccaatc ctgatcataa cggtctgcta cgggctgatg
      781 ttgctgaggc tgaggagtgt gaggctgctg agcctgagct caggcattaa cgtctatatc
      841 aaggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
      901 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
      961 gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac
     1021 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1081 atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg
     1141 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1201 agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1261 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1321 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1381 atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1441 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1501 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cccacatgac
     1561 caactgaaag aaaaagatcg aagtctgcgc cggattacac ggatggttct ggtagtggtg
     1621 ggcgcgttcg tggtgtgttg ggcccccatc cacatattcg tgattgtgtg gacattggtc
     1681 gacattgacc gccgagatcc tctggtggta gctgctctcc atctgtgcat cgccttgggc
     1741 tatgccaata gttctctgaa tccggtgctg tatgccttcc tggacgagaa cttcaagaga
     1801 tgttttcggc agctgtgtag gaagccttgt gggcgacccg acccttcaag cttcagccga
     1861 gctcgcgaag caacagcaag agagagggta accgcctgta ctcccagcga tgggcccggg
     1921 ggtggagccg ccgcgcagtc ccagcccatc ctcaacacca aggagatggc cccgcagagc
     1981 aagcctccag aggagctgga gatgagcagc atgcccagcc ccgtggcccc tctgcccgca
     2041 cgcacggagg gcgtcatcga catgcggagc atgtccagca ttgacagctt catcagctgt
     2101 gccacggact tccctgaagc caccagattc ttctgctacg agaacgaggt gtga
//
