LOCUS       sequence-390413-        2244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express muLight biosensor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Dong C, Gowrishankar R, Jin Y, He XJ, Gupta A, Wang H, Sayar-Atasoy 
            N, Flores RJ, Mahe K, Tjahjono N, Liang R, Marley A, Or Mizuno G, Lo
            DK, Sun Q, Whistler JL, Li B, Gomes I, Von Zastrow M, Tejeda HA, 
            Atasoy D, Devi LA, Bruchas MR, Banghart MR, Tian L
  TITLE     Unlocking opioid neuropeptide dynamics with genetically encoded 
            biosensors.
  JOURNAL   Nat Neurosci. 2024 Jul 15. doi: 10.1038/s41593-024-01697-1.
  PUBMED    39009835
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jul 15. doi: 10.1038/s41593-024-01697-1."
COMMENT     Sequence Label: pCMV-µLight
FEATURES             Location/Qualifiers
     source          1..2244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1104..1125
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1231..1252)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1492..1511)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgaagacga tcatcgccct gagctacatc ttctgcctgg tgttcgccga ctacaaggac
       61 gatgatgacg ccatggatag tagcgctgcg cctaccaacg cgtcaaactg caccgatgct
      121 cttgcgtact cctcctgctc cccggcacct agtcccggtt cttgggtcaa tttgtcccat
      181 ctggacggaa acctctctga tccctgtggg cctaacagga cggacctcgg tgggagggac
      241 tccctttgcc cgccgaccgg atctccgtcc atgataacgg ccattacaat tatggcgttg
      301 tatagcatcg tatgcgttgt aggtcttttt gggaatttcc tggtgatgta cgtcatcgtc
      361 aggtacacaa agatgaaaac agctactaac atttatatat ttaacctggc gctcgcggac
      421 gctctcgcaa cgtcaacgct cccgtttcag tccgtgaatt atctcatggg tacttggcct
      481 ttcggaacaa tactgtgtaa aattgttata agcatagatt attataatat gttcacgtcc
      541 atcttcacac tctgcacaat gtctgtggat aggtacattg ctgtatgtca cccacagaag
      601 gcgcttgact ttagaactcc acgcaatgca aagattataa atgtgtgcaa ctggatcttg
      661 tcctctgcaa tagggcttcc tgtgatgttc atggcgacta ctaagtacag acagggcagc
      721 atagattgca cactcacctt ctcacaccca acttggtact gggaaaatct gctcaagatc
      781 tgcgtcttca tttttgcttt tatcatgcca gtattgataa tcacggtctg ttacgggttg
      841 atgattttgc ggctcaaatc agttagaatg ttgtctggga gcctgagctc atgcattaac
      901 gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
      961 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
     1021 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa
     1081 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1141 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
     1201 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1261 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
     1321 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1381 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1441 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     1501 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1561 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1621 agccatgacc aactgaagga gaaggaccgc aacctcagaa ggataacgag aatggtgctg
     1681 gtcgtagtgg cggttttcat tgtttgttgg acgccaatac acatatacgt gattataaag
     1741 gctctggtga caattcccga aacaacgttt cagacggtct cttggcattt ctgtattgca
     1801 ttggggtaca ctaattcctg ccttaatcct gtattgtacg cctttctgga tgaaaacttt
     1861 aaaagatgtt tccgcgagtt ctgcataccg accagcagca acattgaaca acaaaactcc
     1921 acgcgcatac ggcaaaatac tagggatcac ccgtccactg cgaatactgt agaccgaacg
     1981 aaccatcagt tggagaattt ggaagcggaa actgctcctc tgccacagtc ccagcccatc
     2041 ctcaacacca aggagatggc cccgcagagc aagcctccag aggagctgga gatgagcagc
     2101 atgcccagcc ccgtggcccc tctgcccgca cgcacggagg gcgtcatcga catgcggagc
     2161 atgtccagca ttgacagctt catcagctgt gccacggact tccctgaagc caccagattc
     2221 ttctgctacg agaacgaggt gtga
//
