LOCUS       sequence-390431-       16672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dLrCas9 gene expression activation plasmid in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 16672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL676
FEATURES             Location/Qualifiers
     source          1..16672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5140..5160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5173..5193
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5206..5226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5605..5754
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5767..5916
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      5927..6175
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(6437..6742)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6539..6558)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7225..7249)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7279..7303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8604..9233
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9662..10735
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10801..10995
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11231..11253)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11329..11469
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11384..11403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11484..11501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11655..12243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11735..11754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12330..13124)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13549..13573
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13651..13825
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14915..15591)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14958..14978)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        join(15671..16672,1..996)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
ORIGIN
        1 cccccccccc ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta
       61 gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc
      121 gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt
      181 tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat
      241 tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg
      301 tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg
      361 tctggttggg cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg
      421 gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag
      481 atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg
      541 catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg
      601 ttcattcgtt caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt
      661 ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat
      721 atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc
      781 atatgcagca tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt
      841 atgttttata attattttga tcttgatata cttggatgat ggcatatgca gcagctatat
      901 gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt
      961 cgatgctcac cctgttgttt ggtgttactt ctgcagcctg caggtagatc gctcgtcgac
     1021 atgacaaagc tgaaccagcc atacggcatc ggcctcgcta ttggctcaaa ctctatcggc
     1081 ttcgcggtgg tcgacgccaa ttcccacctc ctgcgcctga agggcgagac agccatcggc
     1141 gccaggctct tccgcgaggg acagtcagcc gcggatcgga ggggctctag gaccacacgg
     1201 aggcgcctct cccggacaag gtggcgcctc agcttcctgc gcgacttctt cgcgccacat
     1261 atcaccaaga ttgaccctga tttcttcctc cgccagaagt actccgagat cagccctaag
     1321 gacaaggatc ggttcaagta cgagaagagg ctgttcaacg accgcaccga tgccgagttc
     1381 tacgaggatt acccatcaat gtaccacctg aggctccatc tgatgaccca cacacataag
     1441 gccgacccga gggagatttt cctcgccatc caccatattc tcaagtctcg gggccacttc
     1501 ctcacacctg gagcggccaa ggatttcaat accgacaagg tggatctgga ggacatcttc
     1561 ccagccctca cagaggcgta cgcccaggtc tacccggacc tggagctgac cttcgacctc
     1621 gcgaaggccg acgatttcaa ggcgaagctc ctggatgagc aggccacccc gtccgacaca
     1681 cagaaggcgc tggtgaacct cctgctctcc agcgatggcg agaaggagat tgtcaagaag
     1741 aggaagcagg tgctgacaga gttcgcgaag gccatcaccg gcctcaagac aaagttcaac
     1801 ctcgccctgg gcaccgaggt ggatgaggcc gatgcctcaa attggcagtt ctctatgggc
     1861 cagctcgacg ataagtggtc caacatcgag acatccatga cagaccaggg caccgagatt
     1921 ttcgagcaga ttcaggagct gtaccgggcg aggctgctca acggcattgt gccagccggc
     1981 atgtccctga gccaggccaa ggtcgccgat tacggccagc acaaggagga cctggagctg
     2041 ttcaagacat acctcaagaa gctgaatgat catgagctgg cgaagaccat ccggggcctc
     2101 tacgacaggt acattaacgg cgacgatgcc aagccgttcc tgcgcgagga cttcgtcaag
     2161 gcgctcacca aggaggtgac agcccatcca aacgaggtgt ccgagcagct gctcaacagg
     2221 atgggccagg ccaatttcat gctcaagcag aggaccaagg ccaacggcgc aatcccaatt
     2281 cagctccagc agcgcgagct ggatcagatc attgcgaacc agagcaagta ctacgactgg
     2341 ctcgccgccc caaatccagt ggaggcacac aggtggaaga tgccgtacca gctcgatgag
     2401 ctgctcaatt tccatatccc atactacgtc ggccctctga ttacaccgaa gcagcaggcg
     2461 gagtcaggcg agaacgtgtt cgcctggatg gtcaggaagg acccgtctgg caacatcacc
     2521 ccatacaatt tcgatgagaa ggtggaccgg gaggcgtcag ccaatacatt catccagagg
     2581 atgaagacca cagataccta cctgattggc gaggacgtgc tccctaagca gtctctgctc
     2641 taccagaagt acgaggtgct caacgagctg aacaatgtcc gcatcaacaa tgagtgcctg
     2701 ggcacagatc agaagcagcg gctcatcagg gaggtgttcg agcggcactc atctgtgacc
     2761 attaagcagg tcgcggataa tctcgtggca catggcgact tcgcccggag gccggagatc
     2821 aggggcctgg cggacgagaa gaggttcctc tccagcctgt ccacatacca ccagctcaag
     2881 gagatcctgc atgaggccat tgacgatcca accaagctgc tcgatatcga gaacatcatt
     2941 acctggagca cagtgttcga ggaccacacc atcttcgaga caaagctggc cgagattgag
     3001 tggctcgacc cgaagaagat caacgagctg tcaggcattc gctaccgggg atggggacag
     3061 ttctctcgca agctgctcga cggcctcaag ctgggcaacg gccacacagt cattcaggag
     3121 ctgatgctga gcaaccataa tctcatgcag atcctggccg atgagacact caaggagaca
     3181 atgacagagc tgaaccagga caagctcaag acagacgata tcgaggatgt gattaacgac
     3241 gcgtacacct caccatctaa taagaaggcc ctccggcagg tcctgagggt ggtcgaggat
     3301 attaagcatg ccgcgaatgg ccaggaccct tcatggctct tcatcgagac agccgacggc
     3361 accggcacag ccggcaagcg cacacagtct cggcagaagc agatccagac cgtgtacgcg
     3421 aacgccgcgc aggagctgat tgattccgcc gtgaggggcg agctggagga taagatcgcg
     3481 gacaaggcct ctttcacaga ccgcctcgtg ctgtacttca tgcagggcgg cagggatatc
     3541 tacacaggcg cccctctgaa tattgaccag ctctcccact acgacatcga tgctattctc
     3601 ccgcagtcac tgattaagga cgattctctg gacaacaggg tgctcgtcaa cgcgacaatc
     3661 aatcgcgaga agaacaatgt gttcgcctcc acactcttcg cgggcaagat gaaggcaacc
     3721 tggcggaagt ggcatgaggc aggcctgatt tcaggacgca agctccggaa tctcatgctg
     3781 aggccggatg agatcgacaa gttcgccaag ggattcgtgg caaggcagct ggtggagaca
     3841 aggcagatca ttaagctcac agagcagatc gctgccgccc agtaccctaa caccaagatc
     3901 attgccgtga aggcgggcct cagccaccag ctgagggagg agctggattt cccgaagaac
     3961 agggacgtga atcattacca ccatgcgttc gatgccttcc tcgccgcaag gattggcaca
     4021 tacctgctca agcgctaccc gaagctggcg cctttcttca cctacggcga gttcgccaag
     4081 gtggacgtga agaagttcag ggagttcaac ttcatcggcg cgctcacaca cgccaagaag
     4141 aatatcattg cgaaggatac cggcgagatt gtgtgggata aggagaggga catccgcgag
     4201 ctggacagga tctacaattt caagcgcatg ctcattaccc atgaggtcta cttcgagaca
     4261 gccgatctct tcaagcagac catctacgcc gcaaaggact ccaaggagag gggcggcagc
     4321 aagcagctga ttcctaagaa gcagggctac ccgacacagg tgtacggcgg atacacccag
     4381 gagtcaggca gctacaatgc gctcgtgcgc gtggccgagg cagataccac agcctaccag
     4441 gtcatcaaga tttccgcgca gaacgcctcc aagatcgcga gcgccaatct caagagcagg
     4501 gagaagggca agcagctgct caatgagatt gtggtcaagc agctggcgaa gcgccggaag
     4561 aactggaagc cttcagccaa ttctttcaag atcgtgattc cgaggttcgg catgggcaca
     4621 ctgttccaga acgccaagta cggcctcttc atggtcaact cagacaccta ctaccgcaat
     4681 taccaggagc tgtggctgtc ccgggagaat cagaagctgc tcaagaagct cttcagcatc
     4741 aagtacgaga agacacagat gaaccacgat gccctccagg tgtacaaggc catcattgac
     4801 caggtcgaga agttcttcaa gctgtacgat atcaatcagt tcagggcgaa gctctccgac
     4861 gccattgagc gcttcgagaa gctgccgatc aacaccgatg gcaataagat tggcaagacc
     4921 gagacactcc ggcagatcct cattggcctc caggcgaacg gcacaaggtc caacgtgaag
     4981 aatctgggca tcaagaccga cctcggcctg ctccaggtcg gcagcggcat caagctcgac
     5041 aaggataccc agattgtcta ccagtcaccg tctggcctgt tcaagaggcg catcccactc
     5101 gccgacctgg gcagttccgg cgggtcctca agggctgatc ctaagaagaa gaggaaggtt
     5161 tctagggcag atccaaagaa gaagcggaag gtgtctagag ctgatccgaa gaagaagcgc
     5221 aaggtgccta ggggaggatc tggtggtctt ttggatccag gaacccctat ggatgctgac
     5281 cttgttgctt cttccaccgt tgtctgggag caggatgccg accctttcgc tggtaccgca
     5341 gacgacttcc cagcttttaa cgaggaagag cttgcttggc tcatggaatt gttgccacag
     5401 ggaggttctg gtggattgtt ggatcctgga actccaatgg acgctgatct tgttgcttcc
     5461 tctactgtcg tttgggagca agacgctgac cctttcgctg gaaccgctga cgatttccca
     5521 gcctttaacg aagaggaatt ggcctggctt atggagttgc ttcctcaggc taggggtgga
     5581 tctggaggtg gtggtagcgg aggagatgct cttgatgatt tcgatcttga tatgcttgga
     5641 tctgacgctc tcgacgactt tgacctcgac atgttgggat ctgatgctct tgacgatttc
     5701 gatttggaca tgttgggttc tgacgccttg gacgatttcg acctcgacat gcttgctagg
     5761 ggatctgacg cccttgatga tttcgacttg gatatgcttg gatctgacgc ccttgatgat
     5821 ttcgacttgg atatgcttgg atctgacgcc cttgatgatt tcgacttgga tatgcttgga
     5881 tctgacgccc ttgatgattt cgacttggat atgctttgac tgcagatatg aagatgaaga
     5941 tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg
     6001 cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag atcacattat
     6061 aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc tcttctgcag
     6121 catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag ataagaggat
     6181 ccgcggatca tgaaccaacg gcctggctgt atttggtggt tgtgtaggga gatggggaga
     6241 agaaaagccc gattctcttc gctgtgatgg gctggatgca tgcgggggag cgggaggccc
     6301 aagtacgtgc acggtgagcg gcccacaggg cgagtgtgag cgcgagaggc gggaggaaca
     6361 gtttagtacc acattgccca gctaactcga acgcgaccaa cttataaacc cgcgcgctgt
     6421 cgcttgtgtg gagaccttat attccccaga acatcaggtt aatggcgttt ttgatgtcat
     6481 tttcgcggtg gctgagatca gccacttctt ccccgataac ggaaaccggc acactggcca
     6541 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac
     6601 gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg
     6661 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat
     6721 aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc
     6781 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg
     6841 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca
     6901 tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct
     6961 tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc
     7021 gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tggtaccggt ctcagtctca
     7081 ggtagatgtc agatcaatca gaaatgattg atctgacatc tacgagttga gatcaaacaa
     7141 agcttcagct gagtttcaat ttctgagccc atgttgggcc atacatatgc cacccgagtg
     7201 caaatcgggt ggcttttttt ctagacccag ctttcttgta caaagttggc attactgcga
     7261 gctcaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     7321 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     7381 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     7441 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     7501 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     7561 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     7621 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     7681 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     7741 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     7801 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     7861 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     7921 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     7981 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     8041 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     8101 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     8161 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     8221 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     8281 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     8341 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     8401 cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     8461 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     8521 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     8581 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     8641 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     8701 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     8761 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     8821 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     8881 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     8941 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     9001 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     9061 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     9121 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     9181 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     9241 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     9301 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     9361 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     9421 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     9481 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     9541 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     9601 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     9661 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     9721 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     9781 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     9841 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     9901 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     9961 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
    10021 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
    10081 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
    10141 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
    10201 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
    10261 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
    10321 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
    10381 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
    10441 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
    10501 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
    10561 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
    10621 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
    10681 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
    10741 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gcctctttcc
    10801 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
    10861 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
    10921 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
    10981 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
    11041 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
    11101 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
    11161 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
    11221 acggtgaaaa cctctgacac atgcagctcc cggaaacggt cacagcttgt ctgtaagcgg
    11281 atgccgggag cagacaagcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
    11341 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    11401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    11461 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    11521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    11581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    11641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    11701 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    11761 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    11821 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    11881 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    11941 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    12001 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    12061 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    12121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    12181 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    12241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    12301 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    12361 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    12421 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    12481 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    12541 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    12601 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    12661 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    12721 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    12781 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    12841 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    12901 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    12961 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    13021 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    13081 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    13141 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    13201 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    13261 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    13321 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    13381 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    13441 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    13501 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    13561 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    13621 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
    13681 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
    13741 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
    13801 ctactcacac attattatgg agaaaataga gcttgtcgat cgttaattaa tcacctccaa
    13861 gatcccggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
    13921 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
    13981 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
    14041 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
    14101 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
    14161 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
    14221 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
    14281 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
    14341 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
    14401 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
    14461 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
    14521 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
    14581 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg
    14641 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
    14701 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
    14761 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga cacgctgtcg
    14821 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatccta
    14881 ggagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg
    14941 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
    15001 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
    15061 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
    15121 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
    15181 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
    15241 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
    15301 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt
    15361 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
    15421 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
    15481 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    15541 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    15601 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    15661 atgggcgcgc ctgcagtgca gcgtgacccg gtcgtgcccc tctcttgaga taaggtgagc
    15721 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt
    15781 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata
    15841 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta
    15901 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt
    15961 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta
    16021 catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt
    16081 ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta
    16141 ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta
    16201 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt
    16261 aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc
    16321 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg
    16381 ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac
    16441 gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat
    16501 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc
    16561 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagaact
    16621 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcs tc
//
