LOCUS       sequence-390434-       17357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 gene expression activation plasmid in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17357)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 17357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL677
FEATURES             Location/Qualifiers
     source          1..17357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2006
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6150..6170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6183..6203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6216..6236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7299..7448
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7461..7610
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      7621..7869
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8131..8436)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8233..8252)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8919..8943)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8973..8997
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10298..10927
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11356..12429
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12495..12689
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12925..12947)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13023..13163
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13078..13097
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13178..13195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13349..13937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13429..13448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14024..14818)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15243..15267
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    15345..15519
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(16609..17285)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(16652..16672)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     1081 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc gttcgtacac
     1141 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1201 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1261 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1321 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1381 cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     1441 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     1501 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     1561 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     1621 caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     1681 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     1741 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1801 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1861 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1921 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1981 cctgttgttt ggtgttactt ctgcagcctg caggtagatc gctcgtcgac atgacaaagc
     2041 tgaaccagcc atacggcatc ggcctcgata ttggctcaaa ctctatcggc ttcgcggtgg
     2101 tcgacgccaa ttcccacctc ctgcgcctga agggcgagac agccatcggc gccaggctct
     2161 tccgcgaggg acagtcagcc gcggatcgga ggggctctag gaccacacgg aggcgcctct
     2221 cccggacaag gtggcgcctc agcttcctgc gcgacttctt cgcgccacat atcaccaaga
     2281 ttgaccctga tttcttcctc cgccagaagt actccgagat cagccctaag gacaaggatc
     2341 ggttcaagta cgagaagagg ctgttcaacg accgcaccga tgccgagttc tacgaggatt
     2401 acccatcaat gtaccacctg aggctccatc tgatgaccca cacacataag gccgacccga
     2461 gggagatttt cctcgccatc caccatattc tcaagtctcg gggccacttc ctcacacctg
     2521 gagcggccaa ggatttcaat accgacaagg tggatctgga ggacatcttc ccagccctca
     2581 cagaggcgta cgcccaggtc tacccggacc tggagctgac cttcgacctc gcgaaggccg
     2641 acgatttcaa ggcgaagctc ctggatgagc aggccacccc gtccgacaca cagaaggcgc
     2701 tggtgaacct cctgctctcc agcgatggcg agaaggagat tgtcaagaag aggaagcagg
     2761 tgctgacaga gttcgcgaag gccatcaccg gcctcaagac aaagttcaac ctcgccctgg
     2821 gcaccgaggt ggatgaggcc gatgcctcaa attggcagtt ctctatgggc cagctcgacg
     2881 ataagtggtc caacatcgag acatccatga cagaccaggg caccgagatt ttcgagcaga
     2941 ttcaggagct gtaccgggcg aggctgctca acggcattgt gccagccggc atgtccctga
     3001 gccaggccaa ggtcgccgat tacggccagc acaaggagga cctggagctg ttcaagacat
     3061 acctcaagaa gctgaatgat catgagctgg cgaagaccat ccggggcctc tacgacaggt
     3121 acattaacgg cgacgatgcc aagccgttcc tgcgcgagga cttcgtcaag gcgctcacca
     3181 aggaggtgac agcccatcca aacgaggtgt ccgagcagct gctcaacagg atgggccagg
     3241 ccaatttcat gctcaagcag aggaccaagg ccaacggcgc aatcccaatt cagctccagc
     3301 agcgcgagct ggatcagatc attgcgaacc agagcaagta ctacgactgg ctcgccgccc
     3361 caaatccagt ggaggcacac aggtggaaga tgccgtacca gctcgatgag ctgctcaatt
     3421 tccatatccc atactacgtc ggccctctga ttacaccgaa gcagcaggcg gagtcaggcg
     3481 agaacgtgtt cgcctggatg gtcaggaagg acccgtctgg caacatcacc ccatacaatt
     3541 tcgatgagaa ggtggaccgg gaggcgtcag ccaatacatt catccagagg atgaagacca
     3601 cagataccta cctgattggc gaggacgtgc tccctaagca gtctctgctc taccagaagt
     3661 acgaggtgct caacgagctg aacaatgtcc gcatcaacaa tgagtgcctg ggcacagatc
     3721 agaagcagcg gctcatcagg gaggtgttcg agcggcactc atctgtgacc attaagcagg
     3781 tcgcggataa tctcgtggca catggcgact tcgcccggag gccggagatc aggggcctgg
     3841 cggacgagaa gaggttcctc tccagcctgt ccacatacca ccagctcaag gagatcctgc
     3901 atgaggccat tgacgatcca accaagctgc tcgatatcga gaacatcatt acctggagca
     3961 cagtgttcga ggaccacacc atcttcgaga caaagctggc cgagattgag tggctcgacc
     4021 cgaagaagat caacgagctg tcaggcattc gctaccgggg atggggacag ttctctcgca
     4081 agctgctcga cggcctcaag ctgggcaacg gccacacagt cattcaggag ctgatgctga
     4141 gcaaccataa tctcatgcag atcctggccg atgagacact caaggagaca atgacagagc
     4201 tgaaccagga caagctcaag acagacgata tcgaggatgt gattaacgac gcgtacacct
     4261 caccatctaa taagaaggcc ctccggcagg tcctgagggt ggtcgaggat attaagcatg
     4321 ccgcgaatgg ccaggaccct tcatggctct tcatcgagac agccgacggc accggcacag
     4381 ccggcaagcg cacacagtct cggcagaagc agatccagac cgtgtacgcg aacgccgcgc
     4441 aggagctgat tgattccgcc gtgaggggcg agctggagga taagatcgcg gacaaggcct
     4501 ctttcacaga ccgcctcgtg ctgtacttca tgcagggcgg cagggatatc tacacaggcg
     4561 cccctctgaa tattgaccag ctctcccact acgacatcga tcatattctc ccgcagtcac
     4621 tgattaagga cgattctctg gacaacaggg tgctcgtcaa cgcgacaatc aatcgcgaga
     4681 agaacaatgt gttcgcctcc acactcttcg cgggcaagat gaaggcaacc tggcggaagt
     4741 ggcatgaggc aggcctgatt tcaggacgca agctccggaa tctcatgctg aggccggatg
     4801 agatcgacaa gttcgccaag ggattcgtgg caaggcagct ggtggagaca aggcagatca
     4861 ttaagctcac agagcagatc gctgccgccc agtaccctaa caccaagatc attgccgtga
     4921 aggcgggcct cagccaccag ctgagggagg agctggattt cccgaagaac agggacgtga
     4981 atcattacca ccatgcgttc gatgccttcc tcgccgcaag gattggcaca tacctgctca
     5041 agcgctaccc gaagctggcg cctttcttca cctacggcga gttcgccaag gtggacgtga
     5101 agaagttcag ggagttcaac ttcatcggcg cgctcacaca cgccaagaag aatatcattg
     5161 cgaaggatac cggcgagatt gtgtgggata aggagaggga catccgcgag ctggacagga
     5221 tctacaattt caagcgcatg ctcattaccc atgaggtcta cttcgagaca gccgatctct
     5281 tcaagcagac catctacgcc gcaaaggact ccaaggagag gggcggcagc aagcagctga
     5341 ttcctaagaa gcagggctac ccgacacagg tgtacggcgg atacacccag gagtcaggca
     5401 gctacaatgc gctcgtgcgc gtggccgagg cagataccac agcctaccag gtcatcaaga
     5461 tttccgcgca gaacgcctcc aagatcgcga gcgccaatct caagagcagg gagaagggca
     5521 agcagctgct caatgagatt gtggtcaagc agctggcgaa gcgccggaag aactggaagc
     5581 cttcagccaa ttctttcaag atcgtgattc cgaggttcgg catgggcaca ctgttccaga
     5641 acgccaagta cggcctcttc atggtcaact cagacaccta ctaccgcaat taccaggagc
     5701 tgtggctgtc ccgggagaat cagaagctgc tcaagaagct cttcagcatc aagtacgaga
     5761 agacacagat gaaccacgat gccctccagg tgtacaaggc catcattgac caggtcgaga
     5821 agttcttcaa gctgtacgat atcaatcagt tcagggcgaa gctctccgac gccattgagc
     5881 gcttcgagaa gctgccgatc aacaccgatg gcaataagat tggcaagacc gagacactcc
     5941 ggcagatcct cattggcctc caggcgaacg gcacaaggtc caacgtgaag aatctgggca
     6001 tcaagaccga cctcggcctg ctccaggtcg gcagcggcat caagctcgac aaggataccc
     6061 agattgtcta ccagtcaccg tctggcctgt tcaagaggcg catcccactc gccgacctgg
     6121 gcagttccgg cgggtcctca agggctgatc ctaagaagaa gaggaaggtt tctagggcag
     6181 atccaaagaa gaagcggaag gtgtctagag ctgatccgaa gaagaagcgc aaggtgccta
     6241 ggggaggatc tggtggtctt ttggatccag gaacccctat ggatgctgac cttgttgctt
     6301 cttccaccgt tgtctgggag caggatgccg accctttcgc tggtaccgca gacgacttcc
     6361 cagcttttaa cgaggaagag cttgcttggc tcatggaatt gttgccacag ggaggttctg
     6421 gtggattgtt ggatcctgga actccaatgg acgctgatct tgttgcttcc tctactgtcg
     6481 tttgggagca agacgctgac cctttcgctg gaaccgctga cgatttccca gcctttaacg
     6541 aagaggaatt ggcctggctt atggagttgc ttcctcaggc taggggagga tctggtggtc
     6601 ttttggatcc aggaacccct atggatgctg accttgttgc ttcttccacc gttgtctggg
     6661 agcaggatgc cgaccctttc gctggtaccg cagacgactt cccagctttt aacgaggaag
     6721 agcttgcttg gctcatggaa ttgttgccac agggaggttc tggtggattg ttggatcctg
     6781 gaactccaat ggacgctgat cttgttgctt cctctactgt cgtttgggag caagacgctg
     6841 accctttcgc tggaaccgct gacgatttcc cagcctttaa cgaagaggaa ttggcctggc
     6901 ttatggagtt gcttcctcag gctaggggag gatctggtgg tcttttggat ccaggaaccc
     6961 ctatggatgc tgaccttgtt gcttcttcca ccgttgtctg ggagcaggat gccgaccctt
     7021 tcgctggtac cgcagacgac ttcccagctt ttaacgagga agagcttgct tggctcatgg
     7081 aattgttgcc acagggaggt tctggtggat tgttggatcc tggaactcca atggacgctg
     7141 atcttgttgc ttcctctact gtcgtttggg agcaagacgc tgaccctttc gctggaaccg
     7201 ctgacgattt cccagccttt aacgaagagg aattggcctg gcttatggag ttgcttcctc
     7261 aggctagggg tggatctgga ggtggtggta gcggaggaga tgctcttgat gatttcgatc
     7321 ttgatatgct tggatctgac gctctcgacg actttgacct cgacatgttg ggatctgatg
     7381 ctcttgacga tttcgatttg gacatgttgg gttctgacgc cttggacgat ttcgacctcg
     7441 acatgcttgc taggggatct gacgcccttg atgatttcga cttggatatg cttggatctg
     7501 acgcccttga tgatttcgac ttggatatgc ttggatctga cgcccttgat gatttcgact
     7561 tggatatgct tggatctgac gcccttgatg atttcgactt ggatatgctt tgactgcaga
     7621 tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg
     7681 tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg
     7741 taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg
     7801 gatctcttct gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt
     7861 aaagataaga ggatccgcgg atcatgaacc aacggcctgg ctgtatttgg tggttgtgta
     7921 gggagatggg gagaagaaaa gcccgattct cttcgctgtg atgggctgga tgcatgcggg
     7981 ggagcgggag gcccaagtac gtgcacggtg agcggcccac agggcgagtg tgagcgcgag
     8041 aggcgggagg aacagtttag taccacattg cccagctaac tcgaacgcga ccaacttata
     8101 aacccgcgcg ctgtcgcttg tgtggagacc ttatattccc cagaacatca ggttaatggc
     8161 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggaaac
     8221 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
     8281 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
     8341 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
     8401 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
     8461 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
     8521 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
     8581 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
     8641 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
     8701 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttggtac
     8761 cggtctcagt ctcaggtaga tgtcagatca atcagaaatg attgatctga catctacgag
     8821 ttgagatcaa acaaagcttc agctgagttt caatttctga gcccatgttg ggccatacat
     8881 atgccacccg agtgcaaatc gggtggcttt ttttctagac ccagctttct tgtacaaagt
     8941 tggcattact gcgagctcaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     9001 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     9061 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     9121 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     9181 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     9241 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     9301 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     9361 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     9421 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     9481 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     9541 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     9601 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     9661 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     9721 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     9781 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     9841 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     9901 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     9961 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
    10021 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
    10081 tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
    10141 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
    10201 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
    10261 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
    10321 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
    10381 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
    10441 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
    10501 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
    10561 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
    10621 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
    10681 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
    10741 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
    10801 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
    10861 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
    10921 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
    10981 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
    11041 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
    11101 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
    11161 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
    11221 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
    11281 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
    11341 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
    11401 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
    11461 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
    11521 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    11581 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    11641 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    11701 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    11761 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    11821 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    11881 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    11941 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    12001 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    12061 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    12121 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    12181 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    12241 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    12301 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    12361 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    12421 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    12481 aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    12541 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    12601 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    12661 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
    12721 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    12781 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    12841 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    12901 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa cggtcacagc
    12961 ttgtctgtaa gcggatgccg ggagcagaca agcgcgtcag cgggtgttgg cgggtgtcgg
    13021 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
    13081 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    13141 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    13201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    13261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    13321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    13381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    13441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    13501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    13561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    13621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    13681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    13741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    13801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    13861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    13921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    13981 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
    14041 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
    14101 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
    14161 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
    14221 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
    14281 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
    14341 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
    14401 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
    14461 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
    14521 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
    14581 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
    14641 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
    14701 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    14761 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    14821 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    14881 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    14941 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    15001 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    15061 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    15121 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    15181 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    15241 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
    15301 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
    15361 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
    15421 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
    15481 cccttatctg ggaactactc acacattatt atggagaaaa tagagcttgt cgatcgttaa
    15541 ttaatcacct ccaagatccc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
    15601 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
    15661 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
    15721 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
    15781 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
    15841 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
    15901 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
    15961 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
    16021 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
    16081 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
    16141 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
    16201 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
    16261 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
    16321 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
    16381 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
    16441 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
    16501 cggacacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
    16561 gctttttcat cctaggagag atagatttgt agagagagac tggtgatttc agcgtgtcct
    16621 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
    16681 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
    16741 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    16801 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
    16861 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
    16921 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    16981 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
    17041 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    17101 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    17161 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    17221 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    17281 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    17341 tgtcgtgctc caccatg
//
