LOCUS       sequence-390440-        9639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of GFP-Rab12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Dhekne HS, Tonelli F, Yeshaw WM, Chiang CY, Limouse C, Jaimon E, 
            Purlyte E, Alessi DR, Pfeffer SR
  TITLE     Genome-wide screen reveals Rab12 GTPase as a critical activator of 
            Parkinson's disease-linked LRRK2 kinase.
  JOURNAL   Elife. 2023 Oct 24;12:e87098. doi: 10.7554/eLife.87098.
  PUBMED    37874635
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-10-24"; volume: "12:e87098. doi"; pages: " 
            10.7554/eLife.87098"
COMMENT     Sequence Label: pMCB306-GFP-Rab12
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        467..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     620..640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             683..863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    910..1035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1532..1765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1950..1994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2292..2409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2662..2695)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2699..2719
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2964..3175
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3176..4114
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4071..4091
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4135..4731
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4135..4154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4631..4651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4741..4794
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4801..4810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4807..4809,4810..4812,4813..5523)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4852..4873)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5113..5132)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5460..5481
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6287..6320)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6376..6964
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6429..6449)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6847..6858)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6967..6983)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6968..6984)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7493..7673
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7735..8323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7815..7834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8494..9285,9286..9354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9117..9136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9355..9459)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9534..9553)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
       61 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      661 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     1381 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1441 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1501 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1561 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1621 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1681 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1741 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1801 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1861 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1921 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1981 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2041 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2101 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2161 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2221 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2281 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2341 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2401 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
     2461 ctctagagat ccgacgcgcc atctctaggc ccgcgccggc cccctcgcac ggacttgtgg
     2521 gagaagctcg gctactcccc tgccccggtt aatttgcata taatatttcc tagtaactat
     2581 agaggcttaa tgtgcgataa aagacagata atctgttctt tttaatacta gctacatttt
     2641 acatgatagg cttggatttc tataacttcg tatagcatac attatacgaa gttataaaca
     2701 gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt gttttgagac tataagtatc
     2761 ccttggagaa ccaccttgtt ggaccaggat gggcaccacc cgtttaagag ctaagctgga
     2821 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2881 tcggtgcttt ttttctcgag tactaggatc cattaggcgg ccgcgtggat aaccgtatta
     2941 ccgccatgca tgtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3001 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     3061 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3121 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     3181 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     3241 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     3301 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     3361 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     3421 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     3481 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     3541 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     3601 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     3661 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     3721 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     3781 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     3841 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     3901 cgattagttc tcgcgctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     3961 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     4021 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     4081 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgagctagcc caccatgacc
     4141 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     4201 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     4261 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4321 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4381 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4441 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4501 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     4561 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     4621 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     4681 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatcggga
     4741 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaccggtc
     4801 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4861 ctggacggcg acgtaaacgg ccacaagttc agcgtgtctg gcgagggcga gggcgatgcc
     4921 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4981 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5041 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5101 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5161 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5221 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5281 aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5341 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5401 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5461 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5521 aaggggcgcg ccatggatcc gggcgccgcg ctgcagaggc gggccggggg cggcggcggt
     5581 ctgggcgcgg gctccccggc gctgtcgggc ggccagggcc gccggaggaa gcagcccccc
     5641 aggccggccg acttcaagtt gcaggtcatc attatcggct cccgcggcgt gggcaagacc
     5701 agcctgatgg agcgcttcac cgacgacacc ttctgcgagg cctgcaagtc caccgtgggt
     5761 gttgacttca aaatcaaaac tgtagagcta agaggaaaga aaattagatt acagatctgg
     5821 gacacagcag gtcaggagag attcaacagc attacctcag cttattacag aagtgccaag
     5881 gggatcatat tagtatatga tatcactaag aaggagacat ttgatgattt gccgaaatgg
     5941 atgaagatga ttgataagta tgcttcagaa gatgcagagc ttctcttagt tggaaataag
     6001 ttggactgtg aaacggacag agaaatcacc aggcagcagg gggaaaagtt tgcacagcag
     6061 atcactggga tgcggttctg tgaagcaagt gccaaggata acttcaatgt ggacgagata
     6121 tttttgaaac ttgtcgatga cattctgaaa aagatgcctc tggatatttt aaggaatgag
     6181 ttgtccaata gtatcctgtc gttacaacca gagcctgaga taccgccaga actgcctcca
     6241 ccaagaccac atgtccgatg ctgttgagaa ttcgtcgagg gacctaataa cttcgtatag
     6301 catacattat acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat
     6361 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6421 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6481 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6541 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6601 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6661 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6721 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6781 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6841 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6901 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6961 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     7021 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7081 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7141 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7201 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7261 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7321 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7381 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7441 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7501 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7561 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7621 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
     7681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8161 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9481 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     9541 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     9601 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaa
//
