LOCUS       sequence-390442-       11367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of PPM1H-mApple.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Dhekne HS, Tonelli F, Yeshaw WM, Chiang CY, Limouse C, Jaimon E, 
            Purlyte E, Alessi DR, Pfeffer SR
  TITLE     Genome-wide screen reveals Rab12 GTPase as a critical activator of 
            Parkinson's disease-linked LRRK2 kinase.
  JOURNAL   Elife. 2023 Oct 24;12:e87098. doi: 10.7554/eLife.87098.
  PUBMED    37874635
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-10-24"; volume: "12:e87098. doi"; pages: " 
            10.7554/eLife.87098"
COMMENT     Sequence Label: CSII-PPM1H D288A-mApple
FEATURES             Location/Qualifiers
     source          1..11367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..22
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(141..161)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        146..473
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      326..459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     388..407
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        521..568
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             587..961
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1091..1212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1128..1147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1182..1201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1261..1277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1261..1277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1274..1296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1285..1301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1309..1315,1316..1333,1334..1339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1354..1375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1492..1509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1663..2251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1743..1762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2422..3213,3214..3282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3045..3064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3283..3387)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3462..3481)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3653..4032
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4034..4232
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4187..4207
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4250..4430
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4477..4602
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5094..5327
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5512..5556
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5830..5947
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6003..7165
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6234..7156
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7113..7133
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8736..9446
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     9149..9168
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9482..10070
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9535..9555)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9953..9964)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10589..10769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10795..10812)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10801..11025
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11071..11367,1..132)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11158..11177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
       61 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      121 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
      181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
      241 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      301 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      361 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctg
      421 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
      481 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
      541 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
      601 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
      661 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
      721 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
      781 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
      841 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
      901 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
      961 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     1021 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     1081 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1141 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1201 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     1261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1441 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     3421 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     3481 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     3541 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     3601 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     3661 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     3721 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3781 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3841 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3901 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3961 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4021 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4081 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4141 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4201 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     4261 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4321 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4381 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     4441 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     4501 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     4561 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     4621 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     4681 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     4741 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     4801 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     4861 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     4921 aagaccaccg cacagcaagg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     4981 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     5041 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     5101 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     5161 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5221 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5281 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     5341 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     5401 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     5461 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     5521 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     5581 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     5641 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     5701 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     5761 ggagagagag acagagacag atccattcga ttagtgaacg gatctacaaa tggcagtatt
     5821 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     5881 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     5941 aaattttcgg gtttattaca gggacagcag aaattcactt tgaattaatt caagcttcgt
     6001 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     6061 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     6121 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     6181 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     6241 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     6301 ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc
     6361 ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     6421 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     6481 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     6541 aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     6601 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     6661 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     6721 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     6781 aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg
     6841 agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc
     6901 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt
     6961 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     7021 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     7081 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     7141 ttttcttcca tttcaggtgt cgtgaacacg ctaccggtct cgagatgctc actcgagtga
     7201 aatctgccgt ggccaatttc atgggcggca tcatggctgg cagctcaggc tccgagcacg
     7261 gcggcggcag ctgcggaggc tcggacctgc ccctgcgttt cccctacggg cggccagagt
     7321 tcctggggct gtctcaggac gaggtggagt gcagcgccga ccacatcgcc cgccccatcc
     7381 tcatcctcaa ggagactcgg cggctgccct gggccactgg ctacgcagag gttatcaatg
     7441 ccgggaagag cacacacaat gaagaccaag ccagctgtga ggtgctcact gtgaagaaga
     7501 aggcaggggc cgtgacctca accccaaaca ggaactcatc caagagacgg tcctcccttc
     7561 ccaatgggga agggctgcag ctgaaggaga actcggaatc cgagggtgtt tcctgccact
     7621 attggtcgct gtttgacggg cacgcggggt ccggggccgc ggtggtggcg tcacgcctgc
     7681 tgcagcacca catcacggag cagctgcagg acatcgtgga catcctgaag aactccgccg
     7741 tcctgccccc tacctgcctg ggggaggagc ctgagaacac gcccgccaac agccggactc
     7801 tgacccgggc agcctccctg cgcggagggg tgggggcccc gggctccccc agcacgcccc
     7861 ccacacgctt ctttaccgag aagaagattc cccatgagtg cctggtcatc ggagcgcttg
     7921 aaagtgcatt caaggaaatg gacctacaga tagaacgaga gaggagttca tataatatat
     7981 ctggtggctg cacggccctc attgtgattt gccttttggg gaagctgtat gttgcaaatg
     8041 ctggggctag cagggccata atcatcagaa atggagaaat tatccccatg tcttcagaat
     8101 ttacccccga gacggagcgc cagcgacttc agtacctggc attcatgcag cctcacttgc
     8161 tgggaaatga gttcacacat ttggagtttc caaggagagt acagagaaag gagcttggaa
     8221 agaagatgct ctacagggac tttaatatga caggctgggc atacaaaacc attgaggatg
     8281 aggacttgaa gttccccctt atatatggag aaggcaagaa ggcccgggta atggcaacta
     8341 ttggagtgac caggggactt ggggaccatg acctgaaggt gcatgactcc aacatctaca
     8401 ttaaaccatt cctgtcttca gctccagagg taagaatcta cgatctttca aaatatgatc
     8461 atggatcaga tgatgtgctg atcttggcca ctgatggact ctgggacgtt ttatcaaatg
     8521 aagaagtagc agaagcaatc actcagtttc ttcctaactg tgatccagat gatcctcaca
     8581 ggtacacact ggcagctcag gacctggtga tgcgtgcccg gggtgtgctg aaggacagag
     8641 gatggcggat atctaatgac cgactgggct caggagacga catttctgta tatgtcattc
     8701 ctttaataca tggaaacaag ctgtcaggaa ttctgatggt gagcaagggc gaggagaata
     8761 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     8821 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg
     8881 ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     8941 agttcatgta cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca
     9001 agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca
     9061 ttattcacgt taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc
     9121 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     9181 aggcctccga ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga
     9241 ggctgaagct gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca
     9301 agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc
     9361 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     9421 gcggcatgga cgagctgtac aagtaatcta gagttaacat cgagggatca agcttatcga
     9481 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9541 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     9601 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     9661 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     9721 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     9781 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     9841 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     9901 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     9961 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
    10021 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
    10081 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
    10141 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
    10201 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10261 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
    10321 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
    10381 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
    10441 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
    10501 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
    10561 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
    10621 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10681 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10741 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
    10801 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10861 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10921 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10981 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    11041 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    11101 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    11161 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11221 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    11281 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    11341 tgacgttgga gtccacgttc tttaata
//
