LOCUS       sequence-390450-       10452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eRF1 (AAQ mutant) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pLX304 3xFlag-eRF1 G183/184A_IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3279..3674
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3734..3851
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3968..4271
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4272..4475
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4426..4446
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4498..4522
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4531..4596
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5909..5933)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5970..6544,6545..6547,6548..6556)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6137..6154)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6364..6383
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6557..7276
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             7279..7320
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7362..7950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7415..7435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7833..7844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8022..8255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8333..8454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8370..8389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8424..8443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8494..8629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8649..8668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8650..8668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8678..8695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8678..8694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8687..8709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8836..9291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8923..8942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9133..9154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9317..9421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9422..9490,9491..10282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9640..9659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag
     3301 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     3361 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     3421 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     3481 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     3541 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     3601 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     3661 atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag
     3721 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     3781 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3841 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     3901 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     3961 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4021 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4081 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4141 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4201 tccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4261 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4321 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4381 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4441 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca
     4501 agtttgtaca aaaaagcagg cttcaccatg gactacaaag accacgacgg tgactataag
     4561 gaccacgaca tcgactacaa agacgatgac gacaaggcgg acgaccccag tgctgccgac
     4621 aggaacgtgg agatctggaa gatcaagaag ctcattaaga gcttggaggc ggcccgcggc
     4681 aatggcacca gcatgatatc attgatcatt cctcccaaag accagatttc acgagtggca
     4741 aaaatgttag cggatgagtt tggaactgca tctaacatta agtcacgagt aaaccgcctt
     4801 tcagtcctgg gagccattac atctgtacaa caaagactca aactttataa caaagtacct
     4861 ccaaatggtc tggttgtata ctgtggaaca attgtaacag aagaaggaaa ggaaaagaaa
     4921 gtcaacattg actttgaacc tttcaaacca attaatacgt cattgtattt gtgtgacaac
     4981 aaattccata cagaggctct tacagcacta ctttcagatg atagcaagtt tggattcatt
     5041 gtaatagatg gtagtggtgc actttttggc acactccaag gaaacacaag agaagtcctg
     5101 cacaaattca ctgtggatct cccaaagaaa cacggtagag ccgctcagtc agccttgcgt
     5161 tttgcccgtt taagaatgga aaagcgacat aactatgttc ggaaagtagc agagactgct
     5221 gtgcagctgt ttatttctgg ggacaaagtg aatgtggctg gtctagtttt agctggatcc
     5281 gctgacttta aaactgaact aagtcaatct gatatgtttg atcagaggtt acaatcaaaa
     5341 gttttaaaat tagttgatat atcctatggt ggtgaaaatg gattcaacca agctattgag
     5401 ttatctactg aagtcctctc caacgtgaaa ttcattcaag agaagaaatt aataggacga
     5461 tactttgatg aaatcagcca ggacacgggc aagtactgtt ttggcgttga agatacacta
     5521 aaggctttgg aaatgggagc tgtagaaatt ctaatagtct atgaaaatct ggatataatg
     5581 agatatgttc ttcattgcca aggcacagaa gaggagaaaa ttctctatct aactccagag
     5641 caagaaaagg ataaatctca tttcacagac aaagagaccg gacaggaaca tgagcttatc
     5701 gagagcatgc ccctgttgga atggtttgct aacaactata aaaaatttgg agctacgttg
     5761 gaaattgtca cagataaatc acaagaaggg tctcagtttg tgaaaggatt tggtggaatt
     5821 ggaggtatct tgcggtaccg agtagatttc cagggaatgg aataccaagg aggagacgat
     5881 gaattttttg accttgatga ctactgagac ccagctttct tgtacaaagt gggaattcgc
     5941 agtcgacggt accgcgggcc cgggatccgc ccctctccct cccccccccc taacgttact
     6001 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atattttatt ttccaccata
     6061 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6121 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6181 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6241 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6301 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6361 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6421 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     6481 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6541 taatatggcc acaaccatgg tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat
     6601 cctgatcgag ctgaatggcg atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga
     6661 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6721 tgtgccctgg cccaccctgg tgaccaccct gagctacggc gtgcagtgct tctcacgcta
     6781 ccccgatcac atgaagcagc acgacttctt caagagcgcc atgcctgagg gctacatcca
     6841 ggagcgcacc atcttcttcg aggatgacgg caactacaag tcgcgcgccg aggtgaagtt
     6901 cgagggcgat accctggtga atcgcatcga gctgaccggc accgatttca aggaggatgg
     6961 caacatcctg ggcaataaga tggagtacaa ctacaacgcc cacaatgtgt acatcatgac
     7021 cgacaaggcc aagaatggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg
     7081 cagcgtgcag ctggccgacc actaccagca gaataccccc atcggcgatg gccctgtgct
     7141 gctgcccgat aaccactacc tgtccaccca gagcgccctg tccaaggacc ccaacgagaa
     7201 gcgcgatcac atgatctact tcggcttcgt gaccgccgcc gccatcaccc acggcatgga
     7261 tgagctgtac aagtgattgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     7321 tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca
     7381 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7441 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7501 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7561 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7621 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7681 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7741 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7801 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7861 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7921 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     7981 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8041 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8101 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8161 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8221 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     8281 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8461 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8521 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     8641 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     8701 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8761 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8821 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8881 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8941 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     9001 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     9061 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     9121 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     9181 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9241 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     9301 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9361 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9421 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9481 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9541 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9601 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9661 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9721 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9781 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9841 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9901 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9961 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10021 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10081 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10141 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10201 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10261 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10321 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10381 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10441 caaaggatct tc
//
