LOCUS       sequence-390454-       11501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. 
            Each gRNA is fused to TLS2 mobility signal that confers graft 
            mobility..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Yang L, Machin F, Wang S, Saplaoura E, Kragler F
  TITLE     Heritable transgene-free genome editing in plants by grafting of 
            wild-type shoots to transgenic donor rootstocks.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8.
  PUBMED    36593415
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8."
COMMENT     Sequence Label: gRNA NIA TLS2
FEATURES             Location/Qualifiers
     source          1..11501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..1173)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(1602..2231)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3532..3556
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     3744..3766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3758..3775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3759..3775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3856..3880
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        4359..4434
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        4981..5056
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5312..5336)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5404..5420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5404..5420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5417..5439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5428..5444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5452..5458,5459..5476,5477..5482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5497..5518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5709..6385
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     6322..6342
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6448..7245
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6505..6524)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7115..7134
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7302..7476
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7554..7578
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8003..8797
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10083..10671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10572..10591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10825..10842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10857..10997
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10923..10942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11083..11105
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      join(11341..11501,1..34)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
       61 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
      121 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
      181 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
      241 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
      301 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
      361 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
      421 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
      481 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
      541 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
      601 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
      661 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
      721 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
      781 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
      841 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
      901 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
      961 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     1021 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     1081 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     1141 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     1201 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
     1261 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     1321 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     1381 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     1441 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     1501 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     1561 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     1621 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     1681 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     1741 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     1801 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     1861 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     1921 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     1981 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     2041 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     2101 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     2161 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     2221 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     2281 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     2341 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     2401 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
     2461 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
     2521 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
     2581 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
     2641 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
     2701 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
     2761 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
     2821 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
     2881 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
     2941 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
     3001 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
     3061 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
     3121 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
     3181 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
     3241 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
     3301 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
     3361 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
     3421 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
     3481 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
     3541 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
     3601 caagctcaag ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3661 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     3721 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     3781 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgggcccc ccctcgaggc
     3841 gcgccaagct atcaaacaag tttgtacaaa aaagcaggct ccaccatggg aaccaattca
     3901 gtcgactgga tcccgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
     3961 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
     4021 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
     4081 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
     4141 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
     4201 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
     4261 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
     4321 gagtcgaagt agtgattgac aacactgctg actctgcagt tttagagcta gaaatagcaa
     4381 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcattatc
     4441 agagtggtgg tgggcccata acccacaggt ccgctctgat aaattttttt tgcaaaattt
     4501 tccagatcga tttcttcttc ctctgttctt cggcgttcaa tttctggggt tttctcttcg
     4561 ttttctgtaa ctgaaaccta aaatttgacc taaaaaaaat ctcaaataat atgattcagt
     4621 ggttttgtac ttttcagtta gttgagtttt gcagttccga tgagataaac caatattaat
     4681 ccaaactact gcagcctgac agacaaatga ggatgcaaac aattttaaag tttatctaac
     4741 gctagctgtt ttgtttcttc tctctggtgc accaacgacg gcgttttctc aatcataaag
     4801 aggcttgttt tacttaaggc caataatgtt gatggatcga aagaagaggg cttttaataa
     4861 acgagcccgt ttaagctgta aacgatgtca aaaacatccc acatcgttca gttgaaaata
     4921 gaagctctgt ttatatattg gtagagtcga ctaagagatt gatgggttac aacgtgaagg
     4981 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     5041 ggcaccgagt cggtgcatta tcagagtggt ggtgggccca taacccacag gtccgctctg
     5101 ataaattttt tttgcaaaat tttccagatc gatttcttct tcctctgttc ttcggcgttc
     5161 aatttctggg gttttctctt cgttttctgt aactgaaacc taaaatttga cctaaaaaaa
     5221 atctcaaata atatgattca gtggttttgt acttttcagt tagttgagtt ttgcagttcc
     5281 gatgagataa accaatactc gagatatcta gacccagctt tcttgtacaa agtggttcga
     5341 taattcttaa ttaactagtt ctagagcggc cgccaccgcg gtggagctcg aattcgtaat
     5401 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5461 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5521 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5581 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg
     5641 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
     5701 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
     5761 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
     5821 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa
     5881 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     5941 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat
     6001 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
     6061 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
     6121 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     6181 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     6241 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     6301 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     6361 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc
     6421 gagctttcgc agatctgtcg atcgaccatg gggattgaac aagatggatt gcacgcaggt
     6481 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6541 tgctctgatg ccgccgtgtt tcggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6601 accgacctgt ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg
     6661 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6721 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6781 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6841 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6901 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6961 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac acatggcgat
     7021 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7081 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7141 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7201 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7261 tcggatcgat cctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt
     7321 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     7381 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     7441 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt
     7501 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     7561 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     7621 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     7681 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     7741 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     7801 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     7861 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     7921 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     7981 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     8041 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     8101 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     8161 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     8221 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     8281 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     8341 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     8401 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     8461 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     8521 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     8581 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     8641 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     8701 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     8761 aataaaatat tatattttac tggatgaatt gttttaggga aggtgcgaat aagcggggaa
     8821 attcttctcg gctgactcag tcatttcatt tcttcatgtt tgagccgatt ttttctcccg
     8881 taaatgcctt gaatcagcct atttagaccg tttcttcgcc atttaaggcg ttatccccag
     8941 tttttagtga gatctctccc actgacgtat catttggtcc gcccgaaaca ggttggccag
     9001 cgtgaataac atcgccagtt ggttatcgtt tttcagcaac cccttgtatc tggctttcac
     9061 gaagccgaac tgtcgcttga tgatgcgaaa tgggtgctcc accctggccc ggatgctggc
     9121 tttcatgtat tcgatgttga tggccgtttt gttcttgcgt ggatgctgtt tcaaggttct
     9181 taccttgccg gggcgctcgg cgatcagcca gtccacatcc acctcggcca gctcctcgcg
     9241 ctgtggcgcc ccttggtagc cggcatcggc tgagacaaat tgctcctctc catgcagcag
     9301 attacccagc tgattgaggt catgctcgtt ggccgcggtg gtgaccaggc tgtgggtcag
     9361 gccactcttg gcatcgacac caatgtgggc cttcatgcca aagtgccact gattgccttt
     9421 cttggtctga tgcatctccg gatcgcgttg ctgctctttg ttcttggtcg agctgggtgc
     9481 ctcaatgatg gtggcatcga ccaaggtgcc ttgagtcatc atgacgcctg cttcggccag
     9541 ccagcgattg atggtcttga acaattggcg ggccagttga tgctgctcca gcaggtggcg
     9601 gaaattcatg atggtggtgc ggtccggcaa ggcgctatcc agggataacc gggcaaacag
     9661 acgcatggag gcgatttcgt acagagcatc ttccatcgcg ccatcgctca ggttgtacca
     9721 atgctgcatg cagtgaatgc gtagcatggt ttccagcgga taaggtcgcc ggccattacc
     9781 agccttgggg taaaacggct cgatgacttc caccatgttt tgccatggca gaatctgctc
     9841 catgcgggac aagaaaatct cttttctggt ctgacggcgc ttactgctga attcactgtc
     9901 ggcgaaggta agttgatgac tcatgatgaa ccctgttcta tggctccaga tgacaaacat
     9961 gatctcatat cagggacttg ttcgcacctt ccttagtacc tagaatgcat gaccaaaatc
    10021 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10081 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10141 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10201 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    10261 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10321 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10381 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10441 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10501 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10561 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10621 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10681 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10741 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10801 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
    10861 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    10921 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
    10981 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
    11041 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
    11101 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
    11161 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
    11221 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
    11281 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
    11341 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
    11401 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
    11461 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt a
//
