LOCUS       sequence-390461-       11403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. 
            Neither gRNA is fused to a mobility signal and are therefore 
            non-mobile..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Yang L, Machin F, Wang S, Saplaoura E, Kragler F
  TITLE     Heritable transgene-free genome editing in plants by grafting of 
            wild-type shoots to transgenic donor rootstocks.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8.
  PUBMED    36593415
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8."
COMMENT     Sequence Label: gRNA NIA Null
FEATURES             Location/Qualifiers
     source          1..11403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..1173)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(1602..2231)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3532..3556
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     3744..3766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3758..3775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3759..3775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3856..3880
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        4359..4434
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        4932..5007
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5214..5238)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5306..5322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5306..5322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5319..5341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5330..5346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5354..5360,5361..5378,5379..5384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5399..5420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5611..6287
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     6224..6244
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6350..7147
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6407..6426)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7017..7036
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7204..7378
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7456..7480
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7905..8699
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9985..10573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10474..10493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10727..10744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10759..10899
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10825..10844)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10985..11007
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      join(11243..11403,1..34)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
       61 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
      121 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
      181 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
      241 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
      301 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
      361 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
      421 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
      481 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
      541 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
      601 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
      661 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
      721 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
      781 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
      841 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
      901 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
      961 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     1021 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     1081 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     1141 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     1201 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
     1261 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     1321 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     1381 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     1441 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     1501 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     1561 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     1621 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     1681 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     1741 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     1801 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     1861 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     1921 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     1981 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     2041 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     2101 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     2161 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     2221 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     2281 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     2341 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     2401 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
     2461 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
     2521 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
     2581 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
     2641 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
     2701 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
     2761 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
     2821 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
     2881 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
     2941 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
     3001 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
     3061 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
     3121 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
     3181 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
     3241 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
     3301 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
     3361 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
     3421 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
     3481 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
     3541 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
     3601 caagctcaag ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3661 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     3721 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     3781 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgggcccc ccctcgaggc
     3841 gcgccaagct atcaaacaag tttgtacaaa aaagcaggct ccaccatggg aaccaattca
     3901 gtcgactgga tcccgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
     3961 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
     4021 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
     4081 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
     4141 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
     4201 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
     4261 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
     4321 gagtcgaagt agtgattgac aacactgctg actctgcagt tttagagcta gaaatagcaa
     4381 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     4441 ttgcaaaatt ttccagatcg atttcttctt cctctgttct tcggcgttca atttctgggg
     4501 ttttctcttc gttttctgta actgaaacct aaaatttgac ctaaaaaaaa tctcaaataa
     4561 tatgattcag tggttttgta cttttcagtt agttgagttt tgcagttccg atgagataaa
     4621 ccaatattaa tccaaactac tgcagcctga cagacaaatg aggatgcaaa caattttaaa
     4681 gtttatctaa cgctagctgt tttgtttctt ctctctggtg caccaacgac ggcgttttct
     4741 caatcataaa gaggcttgtt ttacttaagg ccaataatgt tgatggatcg aaagaagagg
     4801 gcttttaata aacgagcccg tttaagctgt aaacgatgtc aaaaacatcc cacatcgttc
     4861 agttgaaaat agaagctctg tttatatatt ggtagagtcg actaagagat tgatgggtta
     4921 caacgtgaag ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     4981 cttgaaaaag tggcaccgag tcggtgcttt tttttgcaaa attttccaga tcgatttctt
     5041 cttcctctgt tcttcggcgt tcaatttctg gggttttctc ttcgttttct gtaactgaaa
     5101 cctaaaattt gacctaaaaa aaatctcaaa taatatgatt cagtggtttt gtacttttca
     5161 gttagttgag ttttgcagtt ccgatgagat aaaccaatac tcgagatatc tagacccagc
     5221 tttcttgtac aaagtggttc gataattctt aattaactag ttctagagcg gccgccaccg
     5281 cggtggagct cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     5341 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5401 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5521 gctagagcag cttgccaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     5581 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     5641 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     5701 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     5761 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     5821 agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg gagcacgaca
     5881 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     5941 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     6001 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     6061 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     6121 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     6181 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     6241 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
     6301 tctctacaaa tctatctctc tcgagctttc gcagatctgt cgatcgacca tggggattga
     6361 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6421 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg tttcggctgt cagcgcaggg
     6481 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tccaggacga
     6541 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6601 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6661 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6721 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6781 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6841 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6901 tctcgtcgtg acacatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6961 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7021 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7081 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7141 cttctgagcg ggactctggg gttcggatcg atcctctagc tagagtcgat cgacaagctc
     7201 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     7261 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     7321 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
     7381 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     7441 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     7501 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     7561 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     7621 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     7681 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
     7741 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     7801 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     7861 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     7921 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     7981 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     8041 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     8101 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     8161 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     8221 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     8281 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     8341 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     8401 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     8461 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     8521 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     8581 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     8641 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagg
     8701 gaaggtgcga ataagcgggg aaattcttct cggctgactc agtcatttca tttcttcatg
     8761 tttgagccga ttttttctcc cgtaaatgcc ttgaatcagc ctatttagac cgtttcttcg
     8821 ccatttaagg cgttatcccc agtttttagt gagatctctc ccactgacgt atcatttggt
     8881 ccgcccgaaa caggttggcc agcgtgaata acatcgccag ttggttatcg tttttcagca
     8941 accccttgta tctggctttc acgaagccga actgtcgctt gatgatgcga aatgggtgct
     9001 ccaccctggc ccggatgctg gctttcatgt attcgatgtt gatggccgtt ttgttcttgc
     9061 gtggatgctg tttcaaggtt cttaccttgc cggggcgctc ggcgatcagc cagtccacat
     9121 ccacctcggc cagctcctcg cgctgtggcg ccccttggta gccggcatcg gctgagacaa
     9181 attgctcctc tccatgcagc agattaccca gctgattgag gtcatgctcg ttggccgcgg
     9241 tggtgaccag gctgtgggtc aggccactct tggcatcgac accaatgtgg gccttcatgc
     9301 caaagtgcca ctgattgcct ttcttggtct gatgcatctc cggatcgcgt tgctgctctt
     9361 tgttcttggt cgagctgggt gcctcaatga tggtggcatc gaccaaggtg ccttgagtca
     9421 tcatgacgcc tgcttcggcc agccagcgat tgatggtctt gaacaattgg cgggccagtt
     9481 gatgctgctc cagcaggtgg cggaaattca tgatggtggt gcggtccggc aaggcgctat
     9541 ccagggataa ccgggcaaac agacgcatgg aggcgatttc gtacagagca tcttccatcg
     9601 cgccatcgct caggttgtac caatgctgca tgcagtgaat gcgtagcatg gtttccagcg
     9661 gataaggtcg ccggccatta ccagccttgg ggtaaaacgg ctcgatgact tccaccatgt
     9721 tttgccatgg cagaatctgc tccatgcggg acaagaaaat ctcttttctg gtctgacggc
     9781 gcttactgct gaattcactg tcggcgaagg taagttgatg actcatgatg aaccctgttc
     9841 tatggctcca gatgacaaac atgatctcat atcagggact tgttcgcacc ttccttagta
     9901 cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    10141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10741 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    10801 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    10861 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
    10921 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    10981 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
    11041 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
    11101 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
    11161 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
    11221 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
    11281 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
    11341 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
    11401 gta
//
