LOCUS       sequence-390462-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: ROS lux sensor
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      20..106
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(651..1601)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1649..1871
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2369..2463)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2494..3288)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2605..2624)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3215..3234
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             3541..3552
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3559..5001
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             5014..5937
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             5986..7068
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             7083..8066
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             8245..9357
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
       61 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac
      121 ctagggccta gggtacgggt tttgctgccc gcaaacgggc tgttctggtg ttgctagttt
      181 gttatcagaa tcgcagatcc ggcttcagcc ggtttgccgg ctgaaagcgc tatttcttcc
      241 agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct
      301 ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa
      361 caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg
      421 ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag
      481 ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
      541 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
      601 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
      661 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
      721 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
      781 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
      841 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
      901 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
      961 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     1021 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     1081 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     1141 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     1201 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     1261 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     1321 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     1381 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     1441 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     1501 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     1561 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     1621 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     1681 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     1741 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     1801 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     1861 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     1921 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     1981 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     2041 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     2101 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     2161 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     2221 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     2281 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
     2341 tcttactgtc cctagtacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     2401 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     2461 gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     2521 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     2581 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     2641 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     2701 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     2761 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     2821 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     2881 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     2941 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     3001 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     3061 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     3121 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     3181 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     3241 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     3301 tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     3361 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     3421 ttccgacgtc ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat
     3481 agcaatgaac gattatccct atcaagcatt ctgactgagc attgctcaca cgaattcatt
     3541 aaagaggaga aaggtaccat gactaaaaaa atttcattca ttattaacgg ccaggttgaa
     3601 atctttcccg aaggtgatga tttagtgcaa tccattaatt ttggtgataa tagtgtttac
     3661 ctgccaatat tgaatgactc tcatgtaaaa aacattattg attgtaatgg aaataacgaa
     3721 ttacggttgc ataacattgt caattttctc tatacggtag ggcaaagatg gaaaaatgaa
     3781 gaatactcaa gacgcaggac atacattcgt gacttaaaaa aatatatggg atattcagaa
     3841 gaaatggcta agctagaggc caattggata tctatgattt tatgttctaa aggcggcctt
     3901 tatgatgttg tagaaaatga acttggttct cgccatatca tggatgaatg gctacctcag
     3961 gatgaaagtt atgttcgggc ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat
     4021 gttccattat ctgggatcat gtctatatta cgcgcaattt taactaagaa tcagtgtatt
     4081 ataaaaacat cgtcaaccga tccttttacc gctaatgcat tagcgttaag ttttattgat
     4141 gtagacccta atcatccgat aacgcgctct ttatctgtta tatattggcc ccaccaaggt
     4201 gatacatcac tcgcaaaaga aattatgcaa catgcggatg ttattgtcgc ttggggaggg
     4261 ccagatgcga ttaattgggc ggtagagcat gcgccatctt atgctgatgt gattaaattt
     4321 ggttctaaaa agagtctttg cattatcgat aatcctgttg atttgacgtc cgcagcgaca
     4381 ggtgcggctc atgatgtttg tttttacgat cagcgagctt gtttttctgc ccaaaacata
     4441 tattacatgg gaaatcatta tgaggaattt aagttagcgt tgatagaaaa acttaatcta
     4501 tatgcgcata tattaccgaa tgccaaaaaa gattttgatg aaaaggcggc ctattcttta
     4561 gttcaaaaag aaagcttgtt tgctggatta aaagtagagg tggatattca tcaacgttgg
     4621 atgattattg agtcaaatgc aggtgtggaa tttaatcaac cacttggcag atgtgtgtac
     4681 cttcatcacg tcgataatat tgagcaaata ttgccttatg ttcaaaaaaa taagacgcaa
     4741 accatatcta tttttccttg ggagtcatca tttaaatatc gagatgcgtt agcattaaaa
     4801 ggtgcggaaa ggattgtaga agcaggaatg aataacatat ttcgagttgg tggatctcat
     4861 gacggaatgc gaccgttgca acgattagtg acatatattt ctcatgaaag gccatctaac
     4921 tatacggcta aggatgttgc ggttgaaata gaacagactc gattcctgga agaagataag
     4981 ttccttgtat ttgtcccata ataggtaaaa agtatggaaa atgaatcaaa atataaaacc
     5041 atcgaccacg ttatttgtgt tgaaggaaat aaaaaaattc atgtttggga aacgctgcca
     5101 gaagaaaaca gcccaaagag aaagaatgcc attattattg cgtctggttt tgcccgcagg
     5161 atggatcatt ttgctggtct ggcggaatat ttatcgcgga atggatttca tgtgatccgc
     5221 tatgattcgc ttcaccacgt tggattgagt tcagggacaa ttgatgaatt tacaatgtct
     5281 ataggaaagc agagcttgtt agcagtggtt gattggttaa ctacacgaaa aataaataac
     5341 ttcggtatgt tggcttcaag cttatctgcg cggatagctt atgcaagcct atctgaaatc
     5401 aatgcttcgt ttttaatcac cgcagtcggt gttgttaact taagatattc tcttgaaaga
     5461 gctttagggt ttgattatct cagtctaccc attaatgaat tgccgaataa tctagatttt
     5521 gaaggccata aattgggtgc tgaagtcttt gcgagagatt gtcttgattt tggttgggaa
     5581 gatttagctt ctacaattaa taacatgatg tatcttgata taccgtttat tgcttttact
     5641 gcaaataacg ataattgggt caagcaagat gaagttatca cattgttatc aaatattcgt
     5701 agtaatcgat gcaagatata ttctttgtta ggaagttcgc atgacttgag tgaaaattta
     5761 gtggtcctgc gcaattttta tcaatcggtt acgaaagccg ctatcgcgat ggataatgat
     5821 catctggata ttgatgttga tattactgaa ccgtcatttg aacatttaac tattgcgaca
     5881 gtcaatgaac gccgaatgag aattgagatt gaaaatcaag caatttctct gtcttaaaat
     5941 ctattgagat attctatcac tcaaatagca atataaggac tctctatgaa atttggaaac
     6001 tttttgctta cataccaacc tccccaattt tctcaaacag aggtaatgaa acgtttggtt
     6061 aaattaggtc gcatctctga ggagtgtggt tttgataccg tatggttact ggagcatcat
     6121 ttcacggagt ttggtttgct tggtaaccct tatgtcgctg ctgcatattt acttggcgcg
     6181 actaaaaaat tgaatgtagg aactgccgct attgttcttc ccacagccca tccagtacgc
     6241 caacttgaag atgtgaattt attggatcaa atgtcaaaag gacgatttcg gtttggtatt
     6301 tgccgagggc tttacaacaa ggactttcgc gtattcggca cagatatgaa taacagtcgc
     6361 gccttagcgg aatgctggta cgggctgata aagaatggca tgacagaggg atatatggaa
     6421 gctgataatg aacatatcaa gttccataag gtaaaagtaa accccgcggc gtatagcaga
     6481 ggtggcgcac cggtttatgt ggtggctgaa tcagcttcga cgactgagtg ggctgctcaa
     6541 tttggcctac cgatgatatt aagttggatt ataaatacta acgaaaagaa agcacaactt
     6601 gagctttata atgaagtggc tcaagaatat gggcacgata ttcataatat cgaccattgc
     6661 ttatcatata taacatctgt agatcatgac tcaattaaag cgaaagagat ttgccggaaa
     6721 tttctggggc attggtatga ttcttatgtg aatgctacga ctatttttga tgattcagac
     6781 caaacaagag gttatgattt caataaaggg cagtggcgtg actttgtatt aaaaggacat
     6841 aaagatacta atcgccgtat tgattacagt tacgaaatca atcccgtggg aacgccgcag
     6901 gaatgtattg acataattca aaaagacatt gatgctacag gaatatcaaa tatttgttgt
     6961 ggatttgaag ctaatggaac agtagacgaa attattgctt ccatgaagct cttccagtct
     7021 gatgtcatgc catttcttaa agaaaaacaa cgttcgctat tatattagct aaggagaaag
     7081 aaatgaaatt tggattgttc ttccttaact tcatcaattc aacaactgtt caagaacaaa
     7141 gtatagttcg catgcaggaa ataacggagt atgttgataa gttgaatttt gaacagattt
     7201 tagtgtatga aaatcatttt tcagataatg gtgttgtcgg cgctcctctg actgtttctg
     7261 gttttctgct cggtttaaca gagaaaatta aaattggttc attaaatcac atcattacaa
     7321 ctcatcatcc tgtccgcata gcggaggaag cttgcttatt ggatcagtta agtgaaggga
     7381 gatttatttt agggtttagt gattgcgaaa aaaaagatga aatgcatttt tttaatcgcc
     7441 cggttgaata tcaacagcaa ctatttgaag agtgttatga aatcattaac gatgctttaa
     7501 caacaggcta ttgtaatcca gataacgatt tttatagctt ccctaaaata tctgtaaatc
     7561 cccatgctta tacgccaggc ggacctcgga aatatgtaac agcaaccagt catcatattg
     7621 ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat tctaatgatg
     7681 ttagatatga atatgctgaa agatataaag ccgttgcgga taaatatgac gttgacctat
     7741 cagagataga ccatcagtta atgatattag ttaactataa cgaagatagt aataaagcta
     7801 aacaagagac gcgtgcattt attagtgatt atgttcttga aatgcaccct aatgaaaatt
     7861 tcgaaaataa acttgaagaa ataattgcag aaaacgctgt cggaaattat acggagtgta
     7921 taactgcggc taagttggca attgaaaagt gtggtgcgaa aagtgtattg ctgtcctttg
     7981 aaccaatgaa tgatttgatg agccaaaaaa atgtaatcaa tattgttgat gataatatta
     8041 agaagtacca catggaatat acctaataga tttcgagttg cagcgaggcg gcaagtgaac
     8101 gaatccccag gagcatagat aactatgtga ctggggtgag tgaaagcagc caacaaagca
     8161 gcagcttgaa agatgaaggg tataaaagag tatgacagca gtgctgccat actttctaat
     8221 attatcttga ggagtaaaac aggtatgact tcatatgttg ataaacaaga aattacagca
     8281 agctcagaaa ttgatgattt gattttttcg agcgatccat tagtgtggtc ttacgacgag
     8341 caggaaaaaa tcagaaagaa acttgtgctt gatgcatttc gtaatcatta taaacattgt
     8401 cgagaatatc gtcactactg tcaggcacac aaagtagatg acaatattac ggaaattgat
     8461 gacatacctg tattcccaac atcggttttt aagtttactc gcttattaac ttctcaggaa
     8521 aacgagattg aaagttggtt taccagtagc ggcacgaatg gtttaaaaag tcaggtggcg
     8581 cgtgacagat taagtattga gagactctta ggctctgtga gttatggcat gaaatatgtt
     8641 ggtagttggt ttgatcatca aatagaatta gtcaatttgg gaccagatag atttaatgct
     8701 cataatattt ggtttaaata tgttatgagt ttggtggaat tgttatatcc tacgacattt
     8761 accgtaacag aagaacgaat agattttgtt aaaacattga atagtcttga acgaataaaa
     8821 aatcaaggga aagatctttg tcttattggt tcgccatact ttatttattt actctgccat
     8881 tatatgaaag ataaaaaaat ctcattttct ggagataaaa gcctttatat cataaccgga
     8941 ggcggctgga aaagttacga aaaagaatct ctgaaacgtg atgatttcaa tcatctttta
     9001 tttgatactt tcaatctcag tgatattagt cagatccgag atatatttaa tcaagttgaa
     9061 ctcaacactt gtttctttga ggatgaaatg cagcgtaaac atgttccgcc gtgggtatat
     9121 gcgcgagcgc ttgatcctga aacgttgaaa cctgtacctg atggaacgcc ggggttgatg
     9181 agttatatgg atgcgtcagc aaccagttat ccagcattta ttgttaccga tgatgtcggg
     9241 ataattagca gagaatatgg taagtatccc ggcgtgctcg ttgaaatttt acgtcgcgtc
     9301 aatacgagga cgcagaaagg gtgtgcttta agcttaaccg aagcgtttga tagttgaaag
     9361 cttgatatcg aattcctgcc caattattga aggggagcgg gaaaccgctc cccttttttt
     9421 gtttctggtc tccccatg
//
