LOCUS       sequence-390463-        2747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses frame-shifted CI under the control of PLUX promoter, p15A 
            origin of replication, Chloramphenicol selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Sarkar K, Bonnerjee D, Srivastava R, Bagh S
  TITLE     A single layer artificial neural network type architecture with 
            molecular engineered bacteria for reversible and irreversible 
            computing.
  JOURNAL   Chem Sci. 2021 Nov 9;12(48):15821-15832. doi: 10.1039/d1sc01505b. 
            eCollection 2021 Dec 15.
  PUBMED    35024106
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d1sc01505b"; journalName: "Chem Sci"; date: "2021-11-9- 9";
            volume: "12"; issue: "48"; pages: "15821-15832"
COMMENT     Sequence Label: pXC3cIfm
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(25..111)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(123..830)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="TKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQSG
                     VGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYPV
                     FSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGM
                     LILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     RBS             842..853
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     rep_origin      complement(1126..1671)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1206..1225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1785..1879)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1900..2559)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2474..2493
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2560..2662)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttgttcctag gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
       61 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctcta
      121 gatcagccaa acgtctcttc aggccactga ctagcgataa ctttccccac aacggaacaa
      181 ctctcattgc atgggatcat tgggtactgt gggtttagtg gttgtaaaaa cacctgaccg
      241 ctatccctga tcagtttctt gaaggtaaac tcatcacccc caagtctggc tatgcagaaa
      301 tcacctggct caacagcctg ctcagggtca acgagaatta acattccgtc aggaaagctt
      361 ggcttggagc ctgttggtgc ggtcatggaa ttaccttcaa cctcaagcca gaatgcagaa
      421 tcactggctt ttttggttgt gcttacccat ctctccgcat cacctttggt aaaggttcta
      481 agctcaggtg agaacatccc tgcctgaaca tgagaaaaaa cagggtactc atactcactt
      541 ctaagtgacg gctgcatact aaccgcttca tacatctcgt agatttctct ggcgattgaa
      601 gggctaaatt cttcaacgct aactttgaga atttttgcaa gcaatgcggc gttataagca
      661 tttaatgcat tgatgccatt aaataaagca ccaacgcctg actgccccat ccccatcttg
      721 tctgcgacag attcctggga taagccaagt tcatttttct ttttttcata aattgcttta
      781 aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt tcttttttgt gccatggtac
      841 ctttctcctc tttaatgaat tctttattcg actataacaa accattttct tgcgtaaacc
      901 tgtacgatcc tacaggtctc gagttaccag gatcctggca gaagctattc attctgcagt
      961 tccgtgctcc aaaacaaccg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtctg gaagatgcca
     1081 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
     1141 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     1201 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc
     1261 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
     1321 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
     1381 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg
     1441 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca
     1501 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag
     1561 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg
     1621 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat
     1681 cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca
     1741 cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc accaataaaa
     1801 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1861 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1921 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1981 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     2041 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     2101 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     2161 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     2221 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     2281 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     2341 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     2401 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     2461 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     2521 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     2581 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     2641 ggaacctctt acgtgccgat caacgtctca ttttcgccag atatcgacgt ctactgggac
     2701 gaagacgaac agtcgacaac gaagacgcca acatcaagat ctcgcat
//
