LOCUS       sequence-390464-       11570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 11570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pHR RPS27A K113R-3xFlag_IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(906..926)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        911..1240
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1091..1226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2374..2439
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2569..2589
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3014..3148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3051..3070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3105..3124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3316..3949
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3996..4121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4618..4851
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5036..5080
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5365..5482
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5565..6743
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5796..6734
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6691..6711
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6807..7034
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             7275..7340
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(7345..7919,7920..7922,7923..7931)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7512..7529)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7739..7758
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7932..8651
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    8749..9337
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8802..8822)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9220..9231)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9340..9356)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9341..9357)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9447..9680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9705..9723)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9706..9723)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10013..10032)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10246..10265)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10365..10387
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10425..10443)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10511..10615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10616..10684,10685..11476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10834..10853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      901 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
      961 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     1021 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     1081 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1141 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1201 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     1261 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     1321 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     1381 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     1441 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     1501 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     1561 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     1621 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     1681 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     1741 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     1801 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     1861 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     1921 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     1981 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     2041 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     2101 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     2161 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     2221 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     2281 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     2341 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     2401 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     2461 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     2521 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     2581 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     2641 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     2701 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     2761 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     2821 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     2881 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     2941 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3001 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3061 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3121 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     3181 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     3241 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     3301 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     3361 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     3421 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     3481 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     3541 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     3601 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     3661 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     3721 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     3781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     3961 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4021 ctgaagcgcg cacggcaaga ggtgaggggc ggcgactggt gagtacgcca aaaattttga
     4081 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4141 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4201 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     4261 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     4321 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     4381 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     4441 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     4501 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4561 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     4621 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     4681 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     4741 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     4801 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     4861 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     4921 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     4981 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5041 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5101 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5161 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5221 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5281 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     5341 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     5401 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     5461 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     5521 cgataagctt gatatcgaat tcgggccaga tatacgcgtc gtgaggctcc ggtgcccgtc
     5581 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     5641 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     5701 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     5761 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     5821 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     5881 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     5941 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     6001 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     6061 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     6121 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     6181 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     6241 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     6301 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     6361 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     6421 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     6481 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     6541 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     6601 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     6661 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     6721 ttcttccatt tcaggtgtcg tgaggctagc gaattctcac gcgtcaagtg gagcaaggca
     6781 ggtggacagt ggatccccgc ggccgcatgc agattttcgt gaaaaccctt acggggaaga
     6841 ccatcaccct cgaggttgaa ccctcggata cgatagaaaa tgtaaaggcc aagatccagg
     6901 ataaggaagg aattcctcct gatcagcaga gactgatctt tgctggcaag cagctggaag
     6961 atggacgtac tttgtctgac tacaatattc aaaaggagtc tactcttcat cttgtgttga
     7021 gacttcgtgg tggtgctaag aaaaggaaga agaagtctta caccactccc aagaagaata
     7081 agcacaagag aaagaaggtt aagctggctg tcctgaaata ttataaggtg gatgagaatg
     7141 gcaggattag tcgccttcgt cgagagtgcc cttctgatga atgtggtgct ggggtgttta
     7201 tggcaagtca ctttgacaga cattattgtg gcaaatgttg tctgacttac tgtttcaaca
     7261 aaccagaaga caaggactac aaagaccacg acggtgacta taaggaccac gacatcgact
     7321 acaaagacga tgacgacaag taagcccctc tccctccccc ccccctaacg ttactggccg
     7381 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     7441 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     7501 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     7561 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     7621 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     7681 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     7741 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     7801 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     7861 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     7921 tggccacaac catggtgagc aagggcgccg agctgttcac cggcatcgtg cccatcctga
     7981 tcgagctgaa tggcgatgtg aatggccaca agttcagcgt gagcggcgag ggcgagggcg
     8041 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcctgtgc
     8101 cctggcccac cctggtgacc accctgagct acggcgtgca gtgcttctca cgctaccccg
     8161 atcacatgaa gcagcacgac ttcttcaaga gcgccatgcc tgagggctac atccaggagc
     8221 gcaccatctt cttcgaggat gacggcaact acaagtcgcg cgccgaggtg aagttcgagg
     8281 gcgataccct ggtgaatcgc atcgagctga ccggcaccga tttcaaggag gatggcaaca
     8341 tcctgggcaa taagatggag tacaactaca acgcccacaa tgtgtacatc atgaccgaca
     8401 aggccaagaa tggcatcaag gtgaacttca agatccgcca caacatcgag gatggcagcg
     8461 tgcagctggc cgaccactac cagcagaata cccccatcgg cgatggccct gtgctgctgc
     8521 ccgataacca ctacctgtcc acccagagcg ccctgtccaa ggaccccaac gagaagcgcg
     8581 atcacatgat ctacttcggc ttcgtgaccg ccgccgccat cacccacggc atggatgagc
     8641 tgtacaagtg agcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac
     8701 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
     8761 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8821 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8881 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8941 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     9001 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     9061 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     9121 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     9181 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9241 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9301 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg
     9361 aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     9481 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9661 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     9721 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     9781 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     9841 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     9901 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     9961 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
    10021 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
    10081 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
    10141 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
    10201 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
    10261 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    10321 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    10381 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
    10441 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    10501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    11041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
//
