LOCUS       sequence-390466-       12494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNF14 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 12494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pHR V5-RNF14_IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..12494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    850..1083
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1268..1312
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1597..1714
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1797..2975
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2028..2966
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2923..2943
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3042..3083
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(4510..5084,5085..5087,5088..5096)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4677..4694)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4904..4923
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5097..5807
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5242..5260)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5510..5529
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5905..6493
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5958..5978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6376..6387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6496..6512)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6497..6513)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6603..6836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6861..6879)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6862..6879)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7169..7188)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7402..7421)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7521..7543
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7581..7599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7667..7771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7772..7840,7841..8632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7990..8009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8803..9391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9292..9311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9545..9562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9632..9652)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9637..9966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9817..9952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11100..11165
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11295..11315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11740..11874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11777..11796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11831..11850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12042..12494,1..181)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggtgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
      721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
      781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
      841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
      901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
      961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1021 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1081 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1141 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1201 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1261 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1321 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1381 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1441 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     1501 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     1561 tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1621 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1681 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1741 tccagtttgg atcgataagc ttgatatcga attcgggcca gatatacgcg tcgtgaggct
     1801 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     1861 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     1921 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     1981 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2041 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2101 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     2161 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     2221 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     2281 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     2341 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     2401 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     2461 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     2521 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     2581 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     2641 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     2701 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     2761 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     2821 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     2881 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     2941 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcgaattctc acgcgtcaag
     3001 tggagcaagg caggtggaca gtggatcccc gcggccgcat gggtaagcct atccctaacc
     3061 ctctcctcgg tctcgattct acgatgtcgt cagaagatcg agaagctcag gaggatgaat
     3121 tgctggccct ggcaagtatt tacgatggag atgaatttag aaaagcagag tctgtccaag
     3181 gtggagaaac caggatctat ttggatttgc cacagaattt caagatattt gtgagcggca
     3241 attcaaatga gtgtctccag aatagtggct ttgaatacac catttgcttt ctgcctccac
     3301 ttgtgctgaa ctttgaactg ccaccagatt atccatcctc ttccccacct tcattcacac
     3361 ttagtggcaa atggctgtca ccaactcagc tatctgctct atgcaagcac ttagacaacc
     3421 tatgggaaga acaccgtggc agcgtggtcc tgtttgcctg gatgcaattt cttaaggaag
     3481 agaccctagc atacttgaat attgtctctc cttttgagct caagattggt tctcagaaaa
     3541 aagtgcagag aaggacagct caagcttctc ccaacacaga gctagatttt ggaggagctg
     3601 ctggatctga tgtagaccaa gaggaaattg tggatgagag agcagtgcag gatgtggaat
     3661 cactgtcaaa tctgatccag gaaatcttgg actttgatca agctcagcag ataaaatgct
     3721 ttaatagtaa attgttcctg tgcagtatct gtttctgtga gaagctgggt agtgaatgca
     3781 tgtacttctt ggagtgcagg catgtgtact gcaaagcctg tctgaaggac tactttgaaa
     3841 tccagatcag agatggccag gttcaatgcc tcaactgccc agaaccaaag tgcccttcgg
     3901 tggccactcc tggtcaggtc aaagagttag tggaagcaga gttatttgcc cgttatgacc
     3961 gccttctcct ccagtcctcc ttggacctga tggcagatgt ggtgtactgc ccccggccgt
     4021 gctgccagct gcctgtgatg caggaacctg gctgcaccat gggtatctgc tccagctgca
     4081 attttgcctt ctgtactttg tgcaggttga cctaccatgg ggtctcccca tgtaaggtga
     4141 ctgcagagaa attaatggac ttacgaaatg aatacctgca agcggatgag gctaataaaa
     4201 gacttttgga tcaaaggtat ggtaagagag tgattcagaa ggcactggaa gagatggaaa
     4261 gtaaggagtg gctagagaag aactcaaaga gctgcccatg ttgtggaact cccatagaga
     4321 aattagacgg atgtaacaag atgacatgta ctggctgtat gcaatatttc tgttggattt
     4381 gcatgggttc tctctctaga gcaaaccctt acaaacattt caatgaccct ggttcaccat
     4441 gttttaaccg gctgttttat gctgtggatg ttgacgacga tatttgggaa gatgaggtag
     4501 aagactaagc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4561 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4621 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4681 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4741 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4801 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4861 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4921 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4981 gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     5041 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     5101 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     5161 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     5221 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     5281 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     5341 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     5401 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     5461 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     5521 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     5581 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     5641 tcaagaccac ctacaaggcc aagaagcccg tgcagctacc cggcgcctac aacgtcaaca
     5701 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     5761 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcaactc
     5821 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcaggcatgc
     5881 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     5941 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6001 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6061 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6121 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6181 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6241 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6301 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6361 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6421 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6481 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     6541 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6601 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6841 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     6901 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     6961 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     7021 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     7081 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     7141 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     7201 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     7261 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     7321 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     7381 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     7441 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7501 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7561 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7621 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7741 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7801 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7861 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7921 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7981 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8041 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8101 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8161 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8221 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8281 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8341 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8401 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8461 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8521 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8581 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8641 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9421 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9481 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9541 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9601 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     9661 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9721 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9781 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9841 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9901 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9961 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
    10021 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
    10081 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
    10141 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
    10201 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
    10261 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
    10321 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    10381 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
    10441 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    10501 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    10561 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    10621 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    10681 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
    10741 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
    10801 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    10861 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
    10921 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    10981 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
    11041 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
    11101 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
    11161 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
    11221 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
    11281 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
    11341 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
    11401 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
    11461 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
    11521 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
    11581 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
    11641 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    11701 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    11761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    11881 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11941 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    12001 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
    12061 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    12121 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
    12181 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
    12241 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
    12301 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
    12361 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
    12421 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
    12481 tttttgcctg tact
//
