LOCUS       sequence-390467-       12249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNF25 (?RING) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 12249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pHR V5-RNF25 dRING_IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..12249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    850..1083
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1268..1312
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1597..1714
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1797..2975
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2028..2966
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2923..2943
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3042..3083
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(4264..4839,4840..4842,4843..4851)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4432..4449)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4659..4678
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4852..5562
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSQNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4997..5015)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5265..5284
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5660..6248
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5713..5733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6131..6142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6251..6267)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6252..6268)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6358..6591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6616..6634)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6617..6634)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6924..6943)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7157..7176)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7276..7298
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7336..7354)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7422..7526
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7527..7595,7596..8387)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7745..7764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8558..9146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9047..9066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9300..9317
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9387..9407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9392..9721
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9572..9707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10855..10920
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11050..11070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11495..11629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11532..11551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11586..11605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11797..12249,1..181)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggtgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
      721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
      781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
      841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
      901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
      961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1021 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1081 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1141 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1201 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1261 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1321 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1381 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1441 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     1501 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     1561 tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1621 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1681 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1741 tccagtttgg atcgataagc ttgatatcga attcgggcca gatatacgcg tcgtgaggct
     1801 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     1861 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     1921 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     1981 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2041 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2101 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     2161 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     2221 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     2281 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     2341 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     2401 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     2461 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     2521 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     2581 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     2641 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     2701 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     2761 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     2821 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     2881 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     2941 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcgaattctc acgcgtcaag
     3001 tggagcaagg caggtggaca gtggatcccc gcggccgcat gggtaagcct atccctaacc
     3061 ctctcctcgg tctcgattct acgatggcgg cgtctgcgtc tgcagctgca ggggaggagg
     3121 actgggtcct tccctctgaa gttgaagtat tggagtccat ctatctagat gaactacagg
     3181 tgattaaagg aaatggcaga acttcaccat gggagatcta catcactttg catcctgcca
     3241 ctgcagagga ccaggattca cagtatgtct gcttcactct ggtgcttcag gtcccagcag
     3301 agtatcccca tgaggtgcca cagatctcta tccgaaatcc ccgaggactt tcagatgaac
     3361 agatccacac gatcttacag gtgctgggcc acgtggccaa ggctgggctg ggcactgcca
     3421 tgctgtatga actcattgag aaagggaagg aaattctcac agataacaac atccctcatg
     3481 gccagagaga gcccctcgtg tatgatcttg cctcactgaa agcagcccct gaaccccaac
     3541 agcccatgga gctgtaccag cccagtgcag agagcttgcg ccagcaagaa gaacgcaagc
     3601 ggctctacca gaggcagcag gagcgggggg gaatcattga ccttgaggct gagcgaaacc
     3661 gatacttcat cagccttcag cagcctcctg cccctgcgga acctgagtca gctgtagatg
     3721 tctccaaagg atcccaacca cccagcaccc ttgcagcaga actatccacc tcaccagccg
     3781 tccaatccac tttgccacct cctctgcctg tggcgaccca gcacatatgt gagaagattc
     3841 cagggaccag gtcaaatcag caaaggttgg gcgaaaccca gaaagctatg ctagatcccc
     3901 ccaagcccag tcgaggtccc tggcgacagc ccgaacggag gcacccaaag ggaggggagt
     3961 gccacgcccc taaaggtacc cgtgacaccc aggaactgcc acctcctgag gggcccctca
     4021 aggagcccat ggacctaaag ccagaacccc atagccaagg agttgaaggt cctccacaag
     4081 agaaggggcc tggcagctgg caggggcccc caccccgcag gactcgggac tgtgttcgct
     4141 gggagcgctc taaaggccgg acacccggtt cttcctaccc tcgcctgcct cggggccagg
     4201 gagcataccg gcctggtact cggagggagt ccctgggcct ggaatctaag gatggttcct
     4261 aagcccctct ccctcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4321 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4381 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4441 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4501 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4561 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4621 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4681 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4741 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4801 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     4861 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     4921 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     4981 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     5041 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     5101 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     5161 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     5221 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     5281 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     5341 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     5401 accacctaca aggccaagaa gcccgtgcag ctacccggcg cctacaacgt caacatcaag
     5461 ttggacatca cctcccaaaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     5521 ggccgccact ccaccggcgg catggacgag ctgtacaagt aagcggccgc aactcccacc
     5581 tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct
     5641 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5701 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5761 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5821 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5881 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5941 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6001 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6061 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6121 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6181 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6241 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     6301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6361 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     6601 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     6661 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     6721 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     6781 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     6841 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     6901 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     6961 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     7021 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     7081 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     7141 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     7201 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7261 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7321 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7381 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7441 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7501 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7561 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7621 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7681 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7741 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7801 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7861 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7921 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7981 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8041 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8101 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8161 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8221 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8281 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8341 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8401 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8461 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8701 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9181 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9241 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9301 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9361 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     9421 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     9481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9541 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9601 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9661 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9721 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     9781 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     9841 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     9901 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     9961 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
    10021 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
    10081 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
    10141 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
    10201 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
    10261 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
    10321 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
    10381 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
    10441 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
    10501 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
    10561 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
    10621 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
    10681 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
    10741 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
    10801 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
    10861 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
    10921 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
    10981 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
    11041 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
    11101 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
    11161 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
    11221 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
    11281 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
    11341 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    11401 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    11461 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    11521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11581 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11641 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11701 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    11761 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
    11821 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
    11881 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
    11941 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
    12001 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
    12061 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
    12121 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
    12181 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
    12241 gcctgtact
//
