LOCUS       sequence-390470-       12449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNF25 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 12449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pHR V5-RNF25_IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..12449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    850..1083
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1268..1312
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1597..1714
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1797..2975
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2028..2966
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2923..2943
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3042..3083
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(4465..5039,5040..5042,5043..5051)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4632..4649)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4859..4878
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5052..5762
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5197..5215)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5465..5484
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5860..6448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5913..5933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6331..6342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6451..6467)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6452..6468)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6558..6791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6816..6834)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6817..6834)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7124..7143)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7357..7376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7476..7498
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7536..7554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7622..7726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7727..7795,7796..8587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7945..7964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8758..9346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9247..9266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9500..9517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9587..9607)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9592..9921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9772..9907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11055..11120
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11250..11270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11695..11829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11732..11751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11786..11805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11997..12449,1..181)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggtgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
      721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
      781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
      841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
      901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
      961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1021 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1081 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1141 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1201 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1261 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1321 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1381 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1441 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     1501 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     1561 tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1621 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1681 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1741 tccagtttgg atcgataagc ttgatatcga attcgggcca gatatacgcg tcgtgaggct
     1801 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     1861 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     1921 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     1981 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2041 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2101 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     2161 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     2221 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     2281 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     2341 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     2401 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     2461 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     2521 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     2581 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     2641 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     2701 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     2761 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     2821 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     2881 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     2941 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcgaattctc acgcgtcaag
     3001 tggagcaagg caggtggaca gtggatcccc gcggccgcat gggtaagcct atccctaacc
     3061 ctctcctcgg tctcgattct acgatggcgg cgtctgcgtc tgcagctgca ggggaggagg
     3121 actgggtcct tccctctgaa gttgaagtat tggagtccat ctatctagat gaactacagg
     3181 tgattaaagg aaatggcaga acttcaccat gggagatcta catcactttg catcctgcca
     3241 ctgcagagga ccaggattca cagtatgtct gcttcactct ggtgcttcag gtcccagcag
     3301 agtatcccca tgaggtgcca cagatctcta tccgaaatcc ccgaggactt tcagatgaac
     3361 agatccacac gatcttacag gtgctgggcc acgtggccaa ggctgggctg ggcactgcca
     3421 tgctgtatga actcattgag aaagggaagg aaattctcac agataacaac atccctcatg
     3481 gccagtgtgt catctgcctc tatggtttcc aggagaagga ggcctttacc aaaacaccct
     3541 gttaccacta cttccactgc cactgccttg ctcggtacat ccagcacatg gagcaagagc
     3601 tgaaggcaca aggacaggag caggaacagg aacggcagca tgctacaacc aaacagaagg
     3661 cagtcggtgt gcagtgtcca gtgtgcagag agcccctcgt gtatgatctt gcctcactga
     3721 aagcagcccc tgaaccccaa cagcccatgg agctgtacca gcccagtgca gagagcttgc
     3781 gccagcaaga agaacgcaag cggctctacc agaggcagca ggagcggggg ggaatcattg
     3841 accttgaggc tgagcgaaac cgatacttca tcagccttca gcagcctcct gcccctgcgg
     3901 aacctgagtc agctgtagat gtctccaaag gatcccaacc acccagcacc cttgcagcag
     3961 aactatccac ctcaccagcc gtccaatcca ctttgccacc tcctctgcct gtggcgaccc
     4021 agcacatatg tgagaagatt ccagggacca ggtcaaatca gcaaaggttg ggcgaaaccc
     4081 agaaagctat gctagatccc cccaagccca gtcgaggtcc ctggcgacag cccgaacgga
     4141 ggcacccaaa gggaggggag tgccacgccc ctaaaggtac ccgtgacacc caggaactgc
     4201 cacctcctga ggggcccctc aaggagccca tggacctaaa gccagaaccc catagccaag
     4261 gagttgaagg tcctccacaa gagaaggggc ctggcagctg gcaggggccc ccaccccgca
     4321 ggactcggga ctgtgttcgc tgggagcgct ctaaaggccg gacacccggt tcttcctacc
     4381 ctcgcctgcc tcggggccag ggagcatacc ggcctggtac tcggagggag tccctgggcc
     4441 tggaatctaa ggatggttcc taagcccctc tccctccccc ccccctaacg ttactggccg
     4501 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     4561 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4621 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4681 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4741 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4801 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4861 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4921 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     4981 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     5041 tggccacaac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     5101 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     5161 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     5221 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     5281 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     5341 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     5401 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     5461 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     5521 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     5581 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctacccggcg
     5641 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     5701 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     5761 aagcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc
     5821 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     5881 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5941 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6001 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6061 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6121 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6181 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6241 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6301 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6361 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6421 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     6481 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6541 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6601 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6781 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     6841 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     6901 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     6961 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     7021 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     7081 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     7141 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     7201 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     7261 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     7321 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     7381 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7441 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7501 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7561 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9541 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     9601 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9721 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9901 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     9961 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    10021 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    10081 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    10141 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    10201 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    10261 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    10321 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    10381 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    10441 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    10501 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    10561 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    10621 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    10681 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    10741 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    10801 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    10861 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    10921 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    10981 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    11041 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    11101 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    11161 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    11221 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    11281 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    11341 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    11401 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    11461 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    11521 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    11581 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    11641 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    11701 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11761 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11821 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    11881 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    11941 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
    12001 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
    12061 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
    12121 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
    12181 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
    12241 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
    12301 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
    12361 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
    12421 catataagca gctgcttttt gcctgtact
//
