LOCUS       sequence-39049-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-L5-EGFP-L2
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..91
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(95..97,98..100,101..613)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(140..161)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(401..420)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta tacaaaagtt gaccatggtg agcaagggcg aggagctgtt
      121 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      181 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      241 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      301 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      361 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      421 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      481 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      541 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      601 ccacaacatc gaggac
//
