LOCUS       sequence-390492-        5472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homo-dimer expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Petrich A, Aji AK, Dunsing V, Chiantia S
  TITLE     Benchmarking of novel green fluorescent proteins for the 
            quantification of protein oligomerization in living cells.
  JOURNAL   PLoS One. 2023 Aug 3;18(8):e0285486. doi: 
            10.1371/journal.pone.0285486. eCollection 2023.
  PUBMED    37535571
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-08-3"; volume: "18(8):e0285486. doi"; pages: " 
            10.1371/journal.pone.0285486. eCollection 2023"
COMMENT     Sequence Label: Gamillus(2x)
FEATURES             Location/Qualifiers
     source          1..5472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        172..475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     630..650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      718..727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             724..1440
                     /codon_start=1
                     /product="acid-tolerant monomeric green fluorescent protein
                     (Shinoda et al., 2018)"
                     /label=Gamillus
                     /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG
                     RFNMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME
                     GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPNEVKITPHGPNGIR
                     LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHTQIIQKKDPNDTRDHIVQT
                     ELAVAGNLWHGMDELYK"
     CDS             1471..2187
                     /codon_start=1
                     /product="acid-tolerant monomeric green fluorescent protein
                     (Shinoda et al., 2018)"
                     /label=Gamillus
                     /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG
                     RFNMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME
                     GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPNEVKITPHGPNGIR
                     LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHTQIIQKKDPNDTRDHIVQT
                     ELAVAGNLWHGMDELYK"
     misc_feature    2191..2247
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2371..2492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2408..2427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2462..2481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2499..2954)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2636..2657)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2848..2867
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2981..3085
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3087..3444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3087..3278
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3110..3130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3295..3430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3479..4273
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3479..4273
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3533..3552)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4143..4162
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4461..4480)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4505..4552
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4881..5469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5370..5389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
      121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      661 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc
      721 accatggtga gcaagggcga ggaggcatct ggcagagccc tgttccagta ccccatgacc
      781 agcaagatcg agctgaacgg cgagatcaac ggcaagaaat tcaaggtggc cggcgagggc
      841 ttcaccccca gcagcggcag attcaacatg cacgcctact gcaccaccgg cgacctgcct
      901 atgagctggg tcgtgattgc cagccccctc cagtacggct tccacatgtt cgcccactac
      961 cccgaggaca tcacacactt tttccaggaa tgcttccccg gcagctacac cctggaccgg
     1021 accctgagaa tggaaggcga cggcaccctg accacccacc acgagtacag cctggaggac
     1081 ggctgcgtga cctccaagac caccctgaat gccagcggct tcgaccctaa gggcgccacc
     1141 atgaccaaga gcttcgtgaa acaactgcct aacgaggtga agatcacccc ccacggcccc
     1201 aacggcatca gactgaccag caccgtgctg tacctgaagg aggatggcac catccagatc
     1261 ggcacccagg actgcatcgt gacccctgtg ggcggaagga aagtgaccca gcccaaggcc
     1321 cacttcctgc acacccagat catccagaag aaggacccca acgacacccg ggaccacatc
     1381 gtgcagacag aactggccgt ggccggcaat ctgtggcacg gcatggacga gctgtacaag
     1441 tccggaccgc ctgccgctgc accaccggtc atggtgagca agggcgagga ggcatctggc
     1501 agagccctgt tccagtaccc catgaccagc aagatcgagc tgaacggcga gatcaacggc
     1561 aagaaattca aggtggccgg cgagggcttc acccccagca gcggcagatt caacatgcac
     1621 gcctactgca ccaccggcga cctgcctatg agctgggtcg tgattgccag ccccctccag
     1681 tacggcttcc acatgttcgc ccactacccc gaggacatca cacacttttt ccaggaatgc
     1741 ttccccggca gctacaccct ggaccggacc ctgagaatgg aaggcgacgg caccctgacc
     1801 acccaccacg agtacagcct ggaggacggc tgcgtgacct ccaagaccac cctgaatgcc
     1861 agcggcttcg accctaaggg cgccaccatg accaagagct tcgtgaaaca actgcctaac
     1921 gaggtgaaga tcacccccca cggccccaac ggcatcagac tgaccagcac cgtgctgtac
     1981 ctgaaggagg atggcaccat ccagatcggc acccaggact gcatcgtgac ccctgtgggc
     2041 ggaaggaaag tgacccagcc caaggcccac ttcctgcaca cccagatcat ccagaagaag
     2101 gaccccaacg acacccggga ccacatcgtg cagacagaac tggccgtggc cggcaatctg
     2161 tggcacggca tggacgagct gtacaagtaa agatctcgag ctcaagcttc gaattctgca
     2221 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
     2281 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2341 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2461 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     2521 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     2581 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     2641 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     2701 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     2761 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     2821 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     2881 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     2941 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3061 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3121 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3181 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3241 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3301 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3361 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3421 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3481 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3541 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3601 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3661 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     3721 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3781 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3841 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3901 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3961 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4021 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4081 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4141 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4201 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4261 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4321 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4381 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4441 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4501 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     4561 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     4621 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     4681 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     4741 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4801 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4861 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4921 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4981 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5041 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5101 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5461 ctatggaaaa ac
//
