LOCUS       sequence-390496-        2049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 2T with Esp3I expansion point.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-2T-Esp3I
FEATURES             Location/Qualifiers
     source          1..2049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..235
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1303..1891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1792..1811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2045..2049,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt agccaccgtg agacgaacaa
       61 caacgtctct agtgttttgc aggtgtgatg aaaagcctta gggtcaggtg gcacttttcg
      121 gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      181 gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga agagtatgag
      241 tatccaacat ttccgtgtcg cccttattcc cttttttgcg gccttctgcc ttcctgtttt
      301 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcccgagt
      361 gggttacatt gaactggatc tcaacagcgg caaaatctta gagagttttc gccccgaaga
      421 acgcttccca atgatgagca ctttcaaagt tctgctatgt ggcgcggtgt tatcccgtat
      481 tgatgctggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      541 gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgtag
      601 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg
      661 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      721 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      781 agcaatggca acaactctcc gcaaactatt aactggcgaa ctacttactc ttgcttcacg
      841 ccagcaactc attgactgga tggaggcgga caaagttgca ggaccacttc tgcgctcggc
      901 acttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgcgg
      961 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1021 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1081 gattaagcat tggtaactgt cagacgaagg gaaataaata gtagcccgcc tgatgtgcgg
     1141 gctttttttt ggtggtggtt accagtgtcg cttagctacc gtaggtcctc ttaggcggtc
     1201 cgattagcga catagtcatc aatgccttga cggcaaaatc ccttaacgtg agttttcgtt
     1261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1381 ggatcaagag ctactaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1441 aaatactgtt cttctagtgt agccgtagtt atgccaccac ttcaagaact ctgtagcacc
     1501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1621 aacggggggt tcgtgctcac agcccagctt ggagcgaacg acctacaccg aactgagata
     1681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1741 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag tgggaaacgc
     1801 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1861 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1921 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1981 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2041 gcgcagcga
//
