LOCUS       sequence-390497-        2066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 5T with BsaI expansion point.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-5T-BsaI
FEATURES             Location/Qualifiers
     source          1..2066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..235
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1320..1908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1809..1828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2062..2066,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cctcaccgtg agaccaacaa
       61 caaggtctct agtgttttgc aggtgtgatg aaaagcctta gggtcaggtg gcacttttcg
      121 gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      181 gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga agagtattga
      241 aaaaggaaga gtatgagtat ccaacatttc cgtgtcgccc ttattccctt ttttgcggcc
      301 ttctgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      361 cagttgggtg cccgagtggg ttacattgaa ctggatctca acagcggcaa aatcttagag
      421 agttttcgcc ccgaagaacg cttcccaatg atgagcactt tcaaagttct gctatgtggc
      481 gcggtgttat cccgtattga tgctgggcaa gagcaactcg gtcgccgcat acactattct
      541 cagaatgact tggttgagta ttcaccagtc acagaaaagc atcttacgga tggcatgaca
      601 gtaagagaat tatgtagtgc tgccataacc atgagtgata acactgcggc caacttactt
      661 ctgacaacga ttggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      721 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      781 gacaccacga tgcctgtagc aatggcaaca actctccgca aactattaac tggcgaacta
      841 cttactcttg cttcacgcca gcaactcatt gactggatgg aggcggacaa agttgcagga
      901 ccacttctgc gctcggcact tccggctggc tggtttattg ctgataaatc tggagccggt
      961 gagcgtggct ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1021 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1081 gagataggtg cctcactgat taagcattgg taactgtcag acgaagggaa ataaatagta
     1141 gcccgcctga tgtgcgggct tttttttggt ggtggttacc agtgtcgctt agctaccgta
     1201 ggtcctctta ggcggtccga ttagcgacat agtcatcaat gccttgacgg caaaatccct
     1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1381 gcggtggttt gtttgccgga tcaagagcta ctaactcttt ttccgaaggt aactggcttc
     1441 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1621 gcgcagcggt cgggctgaac ggggggttcg tgctcacagc ccagcttgga gcgaacgacc
     1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2041 cgcagccgaa cgaccgagcg cagcga
//
