LOCUS       sequence-390510-        2060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 6 empty.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-6-Empty
FEATURES             Location/Qualifiers
     source          1..2060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..245
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1314..1902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1803..1822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2056..2060,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cggaggcgcg ccggagagat
       61 cgcgcacgcc cgctgagctc ctgcttttgc aggtgtgatg aaaagcctta gggtcaggtg
      121 gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa
      181 atatgtatcc gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga
      241 agagtatgag tatccaacat ttccgtgtcg cccttattcc ctttttttgc ggccttctgc
      301 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      361 ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt
      421 cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg
      481 ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      541 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      601 gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      661 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      721 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      781 acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga actacttact
      841 cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt
      901 ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      961 ggctctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1021 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1081 ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc
     1141 ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct
     1201 cttaggcggt ccgattagcg acatagtcat caatgccttg acggcaaaat cccttaacgt
     1261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1381 gtttgtttgc cggatcaaga gctactaact ctttttccga aggtaactgg cttcagcaga
     1441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1621 cggtcgggct gaacgggggg ttcgtgctca cagcccagct tggagcgaac gacctacacc
     1681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2041 cgaacgaccg agcgcagcga
//
